Abstract:
:We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3'-N-P-P3-P4-M-G-L-5'. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named "yerba mate chlorosis-associated virus" (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Bejerman N,de Breuil S,Debat H,Miretti M,Badaracco A,Nome Cdoi
10.1007/s00705-017-3363-8subject
Has Abstractpub_date
2017-08-01 00:00:00pages
2481-2484issue
8eissn
0304-8608issn
1432-8798pii
10.1007/s00705-017-3363-8journal_volume
162pub_type
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