Abstract:
:Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the developmental processes of renal and hepatic systems as well as of human foetal intestine. Also, BMP-6 induces osteoblastic differentiation of pluripotent mesenchymal cells and is an autocrine stimulator of chondrocyte differentiation. The present study was carried out to investigate the effect of BMP-6 on human cobblestone-area-forming cells (CAFC), that represent the functional primitive repopulating haemopoietic stem cell in long-term bone marrow culture. Also, the effect of BMP-6 on marrow stroma production of interleukin-6, -11 and their common receptor gp130 that is expressed in haemopoietic stem cells and is indispensable for their proliferation and tri-lineage differentiation was examined. Moreover, the effect of BMP-6 on marrow stroma release of soluble adhesion molecule VCAM-1 mediating the primitive haemopoietic stem cell adhesion to marrow stroma was examined. The number of CAFC was significantly reduced after BMP-6 treatment from 88+/-10 per 10(5)cells in control cultures in a dose dependent manner to only 48+/-3 per 10(5)cells in 50 ng/ml BMP-6-treated cultures, P< 0.01. Quantitative ELISA measurement revealed 50 ng/ml BMP-6 was able to significantly reduce IL-6 and IL-11 production from marrow stroma, P< 0.01. Also, BMP-6 significantly increased soluble gp130 release by 7.4-fold in 50 ng/ml BMP-6-treated marrow stroma cultures. The profound rapid increase in this natural antagonist of human IL-6 cytokine family may reduce the gp130 signaling. Also, the soluble VCAM-1 released increased by two-fold in 50 ng/ml BMP-6-treated marrow stroma cultures. The marked increase in the soluble form may exert an antagonist effect on the function of VCAM-1 (ligand for VLA4). Recently, blocking the VLA4/VCAM-1 adhesion pathway was shown to mobilise haemopoietic CD34 positive cells in normal individuals. Also, we previously observed a significantly lower expression of VLA4 (CD49d) on G-CSF-mobilised blood CD34 positive cells than on bone marrow CD34 positive cells before mobilisation in the same normal donors. Since BMP are currently being used in clinical trials for bone repair and fracture healing, the present results suggest a possible role for BMP-6 in mobilising CD34 positive cells for transplantation. Further in vitro tests are required to evaluate this potential mobilising role of BMP-6 in human long-term bone marrow culture.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Ahmed N,Sammons J,Carson RJ,Khokher MA,Hassan HTdoi
10.1006/cbir.2000.0662keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
429-35issue
5eissn
1065-6995issn
1095-8355pii
S1065-6995(00)90662-0journal_volume
25pub_type
杂志文章abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.05.008
更新日期:2005-10-01 00:00:00
abstract::Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0482
更新日期:1999-01-01 00:00:00
abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100314
更新日期:2011-02-01 00:00:00
abstract::Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. Programmed death-ligand 1 (PD-L1) is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11139
更新日期:2019-06-01 00:00:00
abstract::The plasticity of human and murine bone marrow mesenchymal stem cells (BMSCs) has been proven by their ability to trans-differentiate to multilineage cells, including germ cells. We have investigated ability of goat BMSCs to trans-differentiate to germ cells with extrinsic (e.g., retinoic acid [RA] and BMP4 signalling...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10348
更新日期:2015-01-01 00:00:00
abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.04.007
更新日期:2004-01-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.018
更新日期:2009-04-01 00:00:00
abstract::Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10116
更新日期:2013-09-01 00:00:00
abstract::Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prot...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.03.001
更新日期:2007-09-01 00:00:00
abstract::Propofol is one of the extensively commonly used intravenous anaesthetic agents. The effects of Propofol on hepatocellular carcinoma (HCC) growth inhibition and apoptosis have been examined. The techniques used were the MTT assay, flow cytometry, real-time PCR to assess miR-199a expression, as also caspase-8 and caspa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10034
更新日期:2013-03-01 00:00:00
abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10766
更新日期:2017-06-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.011
更新日期:2008-01-25 00:00:00
abstract::Osteocalcin is one of the most abundant non-collagenous proteins in bone that is commonly used as a preliminary biomarker in bone formation. Osteocalcin also regulates energy metabolism as a hormone. Estrogen-related receptor alpha (ERRα) is primarily thought to regulate energy homeostasis through interacting with per...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10148
更新日期:2013-11-01 00:00:00
abstract::The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0207
更新日期:1998-01-01 00:00:00
abstract::To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0099
更新日期:1996-11-01 00:00:00
abstract::microRNAs (miRNAs) have recently been recognized as playing an important role in bone-associated diseases. This study investigated whether the reduced miR-155-5p in steroid-associated osteonecrosis of the femoral head (ONFH) attenuated osteogenic differentiation and cell proliferation by targeting GSK3B. Bone marrow w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11470
更新日期:2021-01-01 00:00:00
abstract::Our aim has been to elucidate the possible mechanism of CJX1, an amlodipine derivative, in the modulation of P-gp function by determining its effect on P-gp ATPase activity. Basal P-gp ATPase activity was increased by CJX1 with half-maximal activity concentration (Km) of 8.6+/-1.4 microM. Kinetic analysis indicated a ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.023
更新日期:2009-10-01 00:00:00
abstract::Neuroblastoma is one of the most common solid tumours in children (8-10% of all malignancies). Over 22% of cases have N-myc amplification associated with aggressively growing neuroblastomas. Oncogene-induced sensitization of cells to apoptosis is an important mechanism for suppression of tumorigenesis. Tumour suppress...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110231
更新日期:2012-04-01 00:00:00
abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.019
更新日期:2008-12-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
更新日期:2016-04-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110183
更新日期:2012-08-01 00:00:00
abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.012
更新日期:2005-02-01 00:00:00
abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11200
更新日期:2019-07-10 00:00:00
abstract::Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinom...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10075
更新日期:2013-06-01 00:00:00
abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
更新日期:1993-04-01 00:00:00
abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0333
更新日期:1999-01-01 00:00:00
abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0371
更新日期:1999-01-01 00:00:00
abstract::Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.02.006
更新日期:2008-07-01 00:00:00