当前位置： SCI文献检索 > CELL BIOLOGY INTERNATIONAL期刊下所有文献 > Comparison of the endogenous IK currents in rat hippocampal neurons and cloned Kv2.1 channels in CHO cells.

Comparison of the endogenous IK currents in rat hippocampal neurons and cloned Kv2.1 channels in CHO cells.

Abstract：

:The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippocampal neurons and the cloned Kv2.1 channel currents in the CHO cells. The slope factors for the activation curves of both currents obtained at different prepulse holding potentials and holding times were similar, suggesting similar voltage-dependent gating. However, the half-maximal activation voltage for I(K) was approximately 20 mV more negative than the Kv2.1 channel in CHO cells at a given prepulse condition, indicating that the neuronal I(K) current had a lower threshold for activation than that of the Kv2.1 channel. In addition, the neuronal I(K) showed a stronger holding membrane potential and holding time-dependence than Kv2.1. The Kv2.1 channel gave a U-shaped inactivation, while the I(K) current did not. The I(K) current also had much stronger voltage-dependent inactivation than Kv2.1. These results imply that the neuronal factors could make Kv2.1 channels easier to activate. The information obtained from these comparative studies help elucidate the mechanism of molecular regulation of the native neuronal I(K) current in neurons.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Liu M,Gong B,Qi Z

doi

10.1016/j.cellbi.2008.08.019

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

1514-20

issue

12

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(08)00549-0

journal_volume

32

pub_type

杂志文章

相关文献

CELL BIOLOGY INTERNATIONAL文献大全
  • Bradykinin receptor-1 activation induces inflammation and increases the permeability of human brain microvascular endothelial cells.

    abstract：:Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11232

    authors： Mugisho OO,Robilliard LD,Nicholson LFB,Graham ES,O'Carroll SJ

    更新日期：2019-09-09 00:00:00

  • Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation.

    abstract：:Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10568

    authors： Wang L,Cheng L,Wang H,Pan H,Yang H,Shao M,Hu T

    更新日期：2016-03-01 00:00:00

  • Effects of human yolk sac endothelial cells on supporting expansion of hematopoietic stem/progenitor cells from cord blood.

    abstract：:In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2006.06.006

    authors： Hu L,Cheng L,Wang J,Zhao H,Duan H,Lu G

    更新日期：2006-11-01 00:00:00

  • Thiazolidinedione induces the adipose differentiation of fibroblast-like cells resident within bovine skeletal muscle.

    abstract：:To investigate the role of peroxisome proliferator-activated receptor gamma (PPARgamma) in adipocyte formation within the skeletal muscle of beef cattle, fibroblast-like cells were isolated from the longissimus muscle of cattle and cultured with activators of murine PPARgammaA thiazolidinedione T-174, which is a speci...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1998.0270

    authors： Torii SI,Kawada T,Matsuda K,Matsui T,Ishihara T,Yano H

    更新日期：1998-01-01 00:00:00

  • In vitro culture and induced differentiation of sheep skeletal muscle satellite cells.

    abstract：:Skeletal muscle satellite cells are adult muscle-derived stem cells receiving increasing attention. Sheep satellite cells have a greater similarity to human satellite cells with regard to metabolism, life span, proliferation and differentiation, than satellite cells of the rat and mouse. We have used 2-step enzymatic ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20110487

    authors： Wu H,Ren Y,Li S,Wang W,Yuan J,Guo X,Liu D,Cang M

    更新日期：2012-06-01 00:00:00

  • Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα-knockdown cells.

    abstract：:Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-rela...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10902

    authors： Yang D,Okamura H,Qiu L

    更新日期：2018-04-01 00:00:00

  • TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

    abstract：:Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10989

    authors： Li L,Qi Y,Ma X,Xiong G,Wang L,Bao C

    更新日期：2018-09-01 00:00:00

  • Magnesium regulates neural stem cell proliferation in the mouse hippocampus by altering mitochondrial function.

    abstract：:In the adult brain, neural stem cells from the subgranular zone (SGZ) of the hippocampus and the subventricular zone (SVZ) of the cortex progress through the following five developmental stages: radial glia-like cells, neural progenitor cells, neuroblasts, immature neurons, and mature neurons. These developmental stag...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10569

    authors： Jia S,Mou C,Ma Y,Han R,Li X

    更新日期：2016-04-01 00:00:00

  • Substrate-induced polarisation of cultured epitheliocytes and fibroblasts: non-reactivity of Ras-transformed cells.

    abstract：:Non-transformed epitheliocytes and fibroblasts undergo modulations between the less polarised and more polarised phenotypes depending on the nature of the substrate. In contrast, transformed cells express polarised phenotype regardless of the substrate. ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2001.0776

    authors： Moizhess TG,Vasiliev JM

    更新日期：2001-01-01 00:00:00

  • Mitochondria, endoplasmic reticulum and actin filament behavior after PDT with chloroaluminum phthalocyanine liposomal in HeLa cells.

    abstract：:Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.04.005

    authors： Maftoum-Costa M,Naves KT,Oliveira AL,Tedesco AC,da Silva NS,Pacheco-Soares C

    更新日期：2008-08-01 00:00:00

  • Vascular endothelial cells and smooth muscle cells mediate carbachol-induced hepatocyte proliferation via muscarinic receptors and IP3/PKC signaling cascades.

    abstract：:An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2009.01.018

    authors： Yoshimura R,Arai J,Endo Y

    更新日期：2009-04-01 00:00:00

  • KRAS G12V mutation upregulates PD-L1 expression via TGF-β/EMT signaling pathway in human non-small-cell lung cancer.

    abstract：:Although clinical data suggest remarkable promise for targeting programmed cell death protein-1 (PD-1) and ligand (PD-L1) signaling in non-small-cell lung cancer (NSCLC), it is still largely undetermined which subtype of patients will be responsive to checkpoint blockade. In the present study, we explored whether PD-L...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11524

    authors： Pan LN,Ma YF,Li Z,Hu JA,Xu ZH

    更新日期：2020-12-15 00:00:00

  • Up-regulation of miR-26a promotes neurite outgrowth and ameliorates apoptosis by inhibiting PTEN in bupivacaine injured mouse dorsal root ganglia.

    abstract：:Local anesthetic of bupivacaine may inhibit neurite outgrowth and induce apoptosis in mouse dorsal root ganglia (DRG) neurons. In this work, we intended to investigate the functional role of microRNA 26a (miR-26a) in regulating bupivacaine-induced nerve injury in DRG neurons. DRG neurons were extracted from C57BL/6 mi...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10461

    authors： Cui C,Xu G,Qiu J,Fan X

    更新日期：2015-08-01 00:00:00

  • Different disturbances--one pathway of protein unfolding. Actin folding-unfolding and misfolding.

    abstract：:This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellbi.2007.01.025

    authors： Povarova OI,Kuznetsova IM,Turoverov KK

    更新日期：2007-04-01 00:00:00

  • Vibration activates the actin/NF-κB axis and upregulates IL-6 and IL-8 expression in human periodontal ligament cells.

    abstract：:We previously reported that mechanical vibration-induced proinflammatory cytokines, interleukin-6 (IL-6) and IL-8, expression in human periodontal ligament (hPDL) cells, however, the underlying mechanism remained unclear. Mechanical stimuli are able to activate cellular responses by inducing the activation of several ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11267

    authors： Phusuntornsakul P,Jitpukdeebodintra S,Pavasant P,Leethanakul C

    更新日期：2020-02-01 00:00:00

  • Influence of membrane physical state on lysosomal potassium ion permeability.

    abstract：:Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2004.12.002

    authors： Wang J,Zhang GJ

    更新日期：2005-06-01 00:00:00

  • Interleukin-6-induced production of type II acute phase proteins and expression of junB gene are downregulated by human recombinant growth hormone in vitro.

    abstract：:Growth hormone (GH), given therapeutically in many human diseases, is able to modulate the maturation and function of many cells of immune system. The present study demonstrates the effect of human recombinant GH on the production of acute phase proteins (APP) as well as on the gene expression of junB proto-oncogene o...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1999.0454

    authors： Derfalvi B,Igaz P,Fulop KA,Szalai C,Falus A

    更新日期：2000-01-01 00:00:00

  • Dendritic cell uptake of iron-based magnetic nanoparticles.

    abstract：:We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no e...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.04.001

    authors： Goya GF,Marcos-Campos I,Fernández-Pacheco R,Sáez B,Godino J,Asín L,Lambea J,Tabuenca P,Mayordomo JI,Larrad L,Ibarra MR,Tres A

    更新日期：2008-08-01 00:00:00

  • Synaptic modulation of neuronal coupling.

    abstract：:Electrotonic coupling among neurons in the vertebrate, and more specifically the mammalian, brain has now been demonstrated to exist in all major brain subdivisions and in the spinal cord. For many of these brain areas, recent studies have investigated the possibilities of modulation of that coupling by synaptically r...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1006/cbir.1998.0386

    authors： Hatton GI

    更新日期：1998-11-01 00:00:00

  • Changes in VEGF expression and DNA synthesis in hepatocytes from hepatectomized and tumour-bearing mice.

    abstract：:Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20090282

    authors： García MN,Andrini LB,Inda AM,Ronderos JR,Hijano JC,Errecalde AL

    更新日期：2010-02-05 00:00:00

  • Hypoxia affects positively the proliferation of bovine satellite cells and their myogenic differentiation through up-regulation of MyoD.

    abstract：:Hypoxia alters the biological functions of skeletal muscle cells to proliferate and differentiate into myotubes. However, the cellular responses of myoblasts to hypoxia differ according to the levels of oxygen and the types of cells studied. This study examined the effect of hypoxia (1% oxygen) on bovine satellite cel...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.03.017

    authors： Kook SH,Son YO,Lee KY,Lee HJ,Chung WT,Choi KC,Lee JC

    更新日期：2008-08-01 00:00:00

  • Enhancement of fertilization by piperine in hamsters.

    abstract：:The effect of piperine on the fertilization of eggs with sperm was investigated in female hamsters. They were intragastrically treated with piperine at doses of 50 and 100 mg/kg BW from day 1 through day 4 of the oestrous cycle. During piperine treatment, these females were superovulated and artificially inseminated (...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1997.0167

    authors： Piyachaturawat P,Pholpramool C

    更新日期：1997-07-01 00:00:00

  • Zebularine significantly sensitises MEC1 cells to external irradiation and radiopharmaceutical therapy when administered sequentially in vitro.

    abstract：:Zebularine is a cytidine analogue incorporated into DNA during replication, inhibiting DNA methyltransferase 1 (DNMT1), resulting in demethylation and changes in gene expression. Such modification may improve radiosensitivity in resistant lymphoma cells. The hypothesis of this study was that zebularine and radiation w...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10215

    authors： Bryan JN,Kumar SR,Jia F,Balkin ER,Lewis MR

    更新日期：2014-02-01 00:00:00

  • Involvement of calpain-I and microRNA34 in kanamycin-induced apoptosis of inner ear cells.

    abstract：:Inner ear cells, including hair cells, spiral ganglion cells, stria vascularis cells and supporting cells on the basilar membrane, play a major role in transducing hearing signals and regulating inner ear homoeostasis. However, their functions are often damaged by antibiotic-induced ototoxicity. Apoptosis is probably ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20100515

    authors： Yu L,Tang H,Jiang XH,Tsang LL,Chung YW,Chan HC

    更新日期：2010-12-01 00:00:00

  • Cytoskeletal actin degradation induced by lovastatin in cardiomyocytes is mediated through caspase-2.

    abstract：:The objective of this study was to test the hypothesis that cytoskeletal actin fragmentation is mediated through caspase-2, specifically examining the ability of a caspase-2 inhibitor to interfere with actin fragmentation, in comparison with a caspase-3 inhibitor. Cardiomyocytes were cultured from embryonic chick hear...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2004.07.012

    authors： Kong JY,Rabkin SW

    更新日期：2004-01-01 00:00:00

  • Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line.

    abstract：:Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, th...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0657

    authors： Kachhap SK,Vetale SP,Dange P,Ghosh SN

    更新日期：2001-01-01 00:00:00

  • Effects of adenosine and epidermal growth factor on the growth of mouse mammary epithelial cells.

    abstract：:The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine at concentrations o...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1998.0231

    authors： Yuh IS,Jang BB,Hong BJ

    更新日期：1998-01-01 00:00:00

  • Automated detection of morphological alterations during apoptosis.

    abstract：:A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell s...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0560

    authors： Lertworasirikul T,Bunyaratvej A,Kubota F

    更新日期：2000-01-01 00:00:00

  • A 160 kDa protein with carbonic anhydrase activity is complexed with rubisco on the outer surface of thylakoids.

    abstract：:This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.01.010

    authors： Lazova GN,Stemler AJ

    更新日期：2008-06-01 00:00:00

  • Phallacidin stains the mitotic spindle of the diatom Pinnularia spp.

    abstract：:Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2004.04.010

    authors： Sampson K

    更新日期：2004-01-01 00:00:00