Abstract:
:Although clinical data suggest remarkable promise for targeting programmed cell death protein-1 (PD-1) and ligand (PD-L1) signaling in non-small-cell lung cancer (NSCLC), it is still largely undetermined which subtype of patients will be responsive to checkpoint blockade. In the present study, we explored whether PD-L1 was regulated by mutant Kirsten rat sarcoma viral oncogene homolog (KRAS), which is frequently mutated in NSCLC and results in poor prognosis and low survival rates. We verified that PD-L1 levels were dramatically increased in KRAS mutant cell lines, particularly in NCI-H441 cells with KRAS G12V mutation. Overexpression of KRAS G12V remarkably elevated PD-L1 messenger RNA and protein levels, while suppression of KRAS G12V led to decreased PD-L1 levels in NCI-H441 cells. Consistently, higher levels of PD-L1 were observed in KRAS-mutated tissues as well as tumor tissues-derived CD4+ and CD8+ T cells using a tumor xenograft in B-NDG mice. Mechanically, both in vitro and in vivo assays found that KRAS G12V upregulated PD-L1 via regulating the progression of epithelial-to-mesenchymal transition (EMT). Moreover, pembrolizumab activated the antitumor activity and decreased tumor growth with KRAS G12V mutated NSCLC. This study demonstrates that KRAS G12V mutation could induce PD-L1 expression and promote immune escape via transforming growth factor-β/EMT signaling pathway in KRAS-mutant NSCLC, providing a potential therapeutic approach for NSCLC harboring KRAS mutations.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Pan LN,Ma YF,Li Z,Hu JA,Xu ZHdoi
10.1002/cbin.11524subject
Has Abstractpub_date
2020-12-15 00:00:00eissn
1065-6995issn
1095-8355pub_type
杂志文章abstract::BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchymal stem cell) in the treatment of lung diseases remains controversial. We have asses...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110447
更新日期:2012-06-01 00:00:00
abstract::Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.015
更新日期:2009-09-01 00:00:00
abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0887
更新日期:2002-01-01 00:00:00
abstract::Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11346
更新日期:2020-07-01 00:00:00
abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10764
更新日期:2017-06-01 00:00:00
abstract::Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11015
更新日期:2018-09-01 00:00:00
abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11509
更新日期:2020-11-28 00:00:00
abstract::Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11232
更新日期:2019-09-09 00:00:00
abstract::Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated wi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11115
更新日期:2019-05-01 00:00:00
abstract::Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.12.002
更新日期:2005-06-01 00:00:00
abstract::Neuroblastoma is one of the most common solid tumours in children (8-10% of all malignancies). Over 22% of cases have N-myc amplification associated with aggressively growing neuroblastomas. Oncogene-induced sensitization of cells to apoptosis is an important mechanism for suppression of tumorigenesis. Tumour suppress...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110231
更新日期:2012-04-01 00:00:00
abstract::A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymph...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1131
更新日期:1995-09-01 00:00:00
abstract::Emergence of resistance to chemotherapy and radiotherapy is a major obstacle for the successful treatment of MM (multiple myeloma). Prednisone, vincristine and melphalan are commonly used chemotherapeutic agents for the treatment of MM. In the current study, we examined the presence of possible cross-resistance betwee...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100061
更新日期:2011-07-01 00:00:00
abstract::The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0583
更新日期:2001-01-01 00:00:00
abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.019
更新日期:2008-12-01 00:00:00
abstract::The number of the nucleoli in a CaCo-2 cell nucleus does not generally depend on the quantity of DNA in the nucleus, but nucleolar DNA content is directly proportional to total nuclear DNA. However, in multinucleolar cells (three or more nucleoli), the nucleolar DNA content increases after 96 h incubation in culture w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.01.004
更新日期:2004-01-01 00:00:00
abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090453
更新日期:2010-10-01 00:00:00
abstract::The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090370
更新日期:2010-10-01 00:00:00
abstract::Following culture of human peripheral blood mononuclear cells (PBMNC) from 25 normal donors and 15 patients with common variable immunodeficiency (CVID), we were unable to identify any IL10-defective patients. Clear-cut effects of IL4 could be demonstrated in controls, while in CVID all effects are less pronounced. Wh...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1030
更新日期:1994-11-01 00:00:00
abstract::Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10454
更新日期:2015-07-01 00:00:00
abstract::It has been shown in many investigations that the abnormally increasing production and deposition of collagen is one of the important mechanisms of pathological scars and other fibrotic diseases [Wang Z, Inokuchi T, Nemoto TK, Uehara M, Baba TT. Antisense oligonucleotide against collagen-specific molecular chaperone 4...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.10.009
更新日期:2008-05-01 00:00:00
abstract::Human myometrial smooth muscle cells (SMCs) were used to evaluate the proliferative activity of lysophosphatidic acid (LPA). This study specifically focuses on the role of Ca(2+)/calmodulin-dependent protein (CaM) kinase and epidermal growth factor (EGF) receptor tyrosine kinase. Myometrial SMCs were cultured from bio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00352-9
更新日期:2003-01-01 00:00:00
abstract::Restriction Endonucleases (REs) may recognize, cleave and remove DNA from fixed chromatin producing specific chromosome banding patterns. However, the modifications produced in the chromatin fibre are not easy to evaluate and compare. The aim of the present investigation was to visualize differences resulting in the t...
journal_title:Cell biology international
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1006/cbir.1996.0026
更新日期:1996-03-01 00:00:00
abstract::A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0917
更新日期:2002-01-01 00:00:00
abstract::The purpose of the study was to examine the effect of interleukins, IL-6, IL-8, and IL-15, on insulin-mediated redistribution of Rab4a, an early endosome marker, in mouse 3T3-L1 adipocytes. The interleukins did not affect cell viability; however, cell number was slightly but significantly higher in cultures exposed to...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10743
更新日期:2017-04-01 00:00:00
abstract::Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10258
更新日期:2014-06-01 00:00:00
abstract::Following genotoxic insult, p53 mutated tumour cells undergo mitotic catastrophe. This is characterised by a switch from mitosis to the endocycle. The essential difference between mitosis and the endocycle is that in the latter, DNA synthesis is uncoupled from cell division, which leads to the formation of endopolyplo...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2005.10.005
更新日期:2005-12-01 00:00:00
abstract::A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10016
更新日期:2013-02-01 00:00:00
abstract::This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.010
更新日期:2008-06-01 00:00:00
abstract::Type I diabetes is characterized by the deficiency of endocrine beta cells in the pancreatic islets of Langerhans and transplantation of islet cells can be an effective therapeutic approach. Embryonic stem cells can be differentiated into any cell type, and therefore represent an unlimited source of islet cells for th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.12.017
更新日期:2008-04-01 00:00:00