Abstract:
:Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation and identified 2 alternatively spliced Fgf8 isoforms, Fgf8a and Fgf8b, among the 8 known splicing isoforms in mammals. The expression of Fgf8a and Fgf8b mRNAs transiently and rapidly increased in the early stage of P19 cells during RA-induced neural differentiation, followed by a decline in expression. The relative amount of Fgf8b was clearly higher than that of Fgf8a at different time-points measured within 24h after RA treatment. Increased Fgf8b mRNA expression was cellular-aggregation dependent. The results demonstrated that cellular-aggregation-induced Fgf8b, but not Fgf8a, may play a pivotal role in early neural differentiation of P19 cells.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Alam AH,Suzuki H,Tsukahara Tdoi
10.1016/j.cellbi.2009.06.015subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1032-7issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(09)00135-8journal_volume
33pub_type
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