Abstract:
:Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the polymerization kinetics of actin under these conditions. Viscometric measurements indicate that the presence of various mono- and divalent cations, ionic strength, and alpha-actinin in combination are responsible for changes in the mechanical properties of solutions containing actin. The actin filament dynamic behavior is drastically reduced under these conditions as confirmed by quasi-elastic light scattering.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Goldmann WHdoi
10.1006/cbir.2002.0898keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
541-6issue
6eissn
1065-6995issn
1095-8355pii
S106569950290898Xjournal_volume
26pub_type
杂志文章abstract::Zinc-finger E-box binding homeobox 1 (ZEB1) is a master regulator of epithelial-mesenchymal transition (EMT) and has been implicated in primary epithelial cancer biological processes, such as invasion and metastasis. However, the role of ZEB1 in progression of melanoma and cancer stem cells (CSCs) remains obscure. In ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10223
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abstract::The cytosolic lipid droplets (cLDs) store excess intracellular lipids, and perilipin-2 is believed to protect cLDs from degradation. Here, we investigated the role of the small G-protein Arf1 and the proteasome in the fates of perilipin-2 and cLDs. In oleate-loaded cells, upon brefeldin A (BFA) treatment, perilipin-2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10547
更新日期:2016-02-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
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abstract::Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10776
更新日期:2017-07-01 00:00:00
abstract::Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0760
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abstract::Inflammatory cytokines exert different effects on hematopoietic stem cells (HSCs), lead to the development of various cell lineages in bone marrow (BM) and are thus a differentiation axis for HSCs. The content used in this article has been obtained by searching PubMed database and Google Scholar search engine of Engli...
journal_title:Cell biology international
pub_type: 杂志文章,评审
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abstract::The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry...
journal_title:Cell biology international
pub_type: 杂志文章
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abstract::Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1070
更新日期:1993-03-01 00:00:00
abstract::Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated wi...
journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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abstract::Osteocalcin is one of the most abundant non-collagenous proteins in bone that is commonly used as a preliminary biomarker in bone formation. Osteocalcin also regulates energy metabolism as a hormone. Estrogen-related receptor alpha (ERRα) is primarily thought to regulate energy homeostasis through interacting with per...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10148
更新日期:2013-11-01 00:00:00
abstract::Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the under...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10954
更新日期:2018-07-01 00:00:00
abstract::Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1029
更新日期:1994-11-01 00:00:00
abstract::Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10989
更新日期:2018-09-01 00:00:00
abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100528
更新日期:2011-07-01 00:00:00
abstract::Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.03.010
更新日期:2007-09-01 00:00:00
abstract::Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00157-4
更新日期:2003-01-01 00:00:00
abstract::Fibrillin-1 is the major component of extracellular matrix microfibrils. Microfibrils dysfunction is responsible for the onset of various connective tissue diseases, including Marfan syndrome. Although ADAMTSL (a disintegrin and metalloproteinase with thrombospondin motifs-like) 6β is one of the fibrillin-1 binding pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11337
更新日期:2020-07-01 00:00:00
abstract::X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC...
journal_title:Cell biology international
pub_type: 杂志文章
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abstract::The effect of an organochlorine pesticide benzene hexachloride (containing alpha, beta, gamma and delta isomers) on the phagocytic activity of the vegetative cells of Dictyostelium discoideum was investigated. Benzene hexachloride (BHC) at concentrations of 60 ppm and above inhibited the phagocytic activity as reveale...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1071
更新日期:1993-03-01 00:00:00
abstract::Ras/myc-transformed serum-free mouse embryo (ras/myc SFME) cells were treated with interferon-gamma (IFN-gamma; 100 U/ml) and/or lipopolysaccharide (LPS; 0.5 microg/ml) for 24 h to simulate inflammatory and infectious conditions and investigate their effects on the expression of inducible nitric oxide synthase (iNOS) ...
journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
abstract::Immunocytochemical staining of citrulline-containing proteins, i.e. products of endogenous peptidylarginine deiminase (EC 3.5.3.15) reaction, has been performed by chemical modification of citrulline residues in situ, followed by probing with IgG specific to the modified residues. Rat spinal cords that had been preinc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1094
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abstract::Resistance to radiotherapy and chemotherapy has been a major problem of conventional cancer therapies, which consequently leads to cancer relapse and cancer-related death. It is known that cancer stem cells (CSCs) play a key role in therapy resistance and CSC-based targeted therapies have been considered as a powerful...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11456
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abstract::In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...
journal_title:Cell biology international
pub_type: 杂志文章
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abstract::Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11241
更新日期:2020-02-01 00:00:00
abstract::Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the development...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0662
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abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
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abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10568
更新日期:2016-03-01 00:00:00
abstract::The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10006
更新日期:2013-01-01 00:00:00