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Quantification of annexin I in subcellular fractions of human neutrophils reveals an exclusive cytosolic localisation.

Abstract：

:Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory gelatinase granules of human neutrophils, suggesting that the protein might be released through a granule mobilisation and fusion process resembling classical secretion. In this study we have determined the intracellular localisation of annexin I in human neutrophils using subcellular fractionation, protein separation by SDS-PAGE and immunoblotting, and show that virtually all annexin I is localised in the cell cytosol.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Movitz C,Dahlgren C

doi

10.1006/cbir.2001.0760

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

963-9

issue

10

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(01)90760-7

journal_volume

25

pub_type

杂志文章

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