Low-dose aspirin promotes endothelial progenitor cell migration and adhesion and prevents senescence.

Abstract:

:Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis and endothelial nitric oxide synthase (eNOS) expression. EPC migration was detected by a modified Boyden chamber assay. EPC adhesion assay was performed by counting adherent cells on fibronectin-coated culture dishes. EPC senescence was assessed by both senescence-associated-beta-galactosidase staining and DAPI staining. EPC proliferation was analyzed by MTT assay. EPC apoptosis was evaluated by flow cytometric analysis. eNOS protein expression was measured by Western blotting analysis. Aspirin promoted EPC migratory and adhesive capacity at concentrations between 0.1 and 100micromol/L and prevented senescence at concentrations between 50 and 100micromol/L. Meanwhile, aspirin in a range of these concentrations did not affect EPC proliferation, apoptosis or eNOS expression. Our findings indicate that low-dose aspirin promotes migration and adhesion and delays the onset of senescence of EPCs.

journal_name

Cell Biol Int

authors

Hu Z,Zhang F,Yang Z,Zhang J,Zhang D,Yang N,Zhang Y,Cao K

doi

10.1016/j.cellbi.2008.03.004

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

761-8

issue

7

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(08)00361-2

journal_volume

32

pub_type

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