Abstract:
:Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: macropexophagy and micropexophagy. Previous studies showed that peroxisomes damaged by a prolonged exposition to PA1 are eliminated by macropexophagy. In this work, Candida boidinii was grown in methanol-containing media, and PA1 was added to the cultures at 2 µg/mL after they reached the mid-exponential growth phase. Samples were taken at 5, 10, 15, 20, and 25 min after the addition of PA1 and processed for ultrastructural analysis. Typical morphological characteristics of micropexophagy were observed: the direct engulfment of peroxisomes by the vacuolar membrane and the presence of the micropexophagic membrane apparatus (MIPA), which mediates the fusion between the opposing tips of the vacuole to complete sequestration of peroxisomes from the cytosol. In conclusion, here we report that, in addition to macropexophagy, peroxisomes damaged by PA1 can be eliminated by micropexophagy. This information is useful to deepen the knowledge of the mechanism of action of PA1 and of that of pexophagy per se.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Ortega-Martínez M,Gutiérrez-Dávila V,Niderhauser-García A,Salazar-Aranda R,Solís-Soto JM,Montes-de-Oca-Luna R,Jaramillo-Rangel Gdoi
10.1002/cbin.11280subject
Has Abstractpub_date
2020-03-01 00:00:00pages
918-923issue
3eissn
1065-6995issn
1095-8355journal_volume
44pub_type
杂志文章abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...
journal_title:Cell biology international
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journal_title:Cell biology international
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pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
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journal_title:Cell biology international
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