Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy.

Abstract:

:Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: macropexophagy and micropexophagy. Previous studies showed that peroxisomes damaged by a prolonged exposition to PA1 are eliminated by macropexophagy. In this work, Candida boidinii was grown in methanol-containing media, and PA1 was added to the cultures at 2 µg/mL after they reached the mid-exponential growth phase. Samples were taken at 5, 10, 15, 20, and 25 min after the addition of PA1 and processed for ultrastructural analysis. Typical morphological characteristics of micropexophagy were observed: the direct engulfment of peroxisomes by the vacuolar membrane and the presence of the micropexophagic membrane apparatus (MIPA), which mediates the fusion between the opposing tips of the vacuole to complete sequestration of peroxisomes from the cytosol. In conclusion, here we report that, in addition to macropexophagy, peroxisomes damaged by PA1 can be eliminated by micropexophagy. This information is useful to deepen the knowledge of the mechanism of action of PA1 and of that of pexophagy per se.

journal_name

Cell Biol Int

authors

Ortega-Martínez M,Gutiérrez-Dávila V,Niderhauser-García A,Salazar-Aranda R,Solís-Soto JM,Montes-de-Oca-Luna R,Jaramillo-Rangel G

doi

10.1002/cbin.11280

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

918-923

issue

3

eissn

1065-6995

issn

1095-8355

journal_volume

44

pub_type

杂志文章
  • Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

    abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.006

    authors: Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha A

    更新日期:2007-03-01 00:00:00

  • Expression of primary cilia in mammalian cells.

    abstract::Mammalian cells in vivo frequently express primary cilia. Although some fully differentiated cell types rarely, if ever, express them, most do, indicating that they are regular cell organelles. Their expression can also be explored in vitro, where conditions--physical and chemical, intrinsic and extrinsic--permit expe...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1006/cbir.1996.0011

    authors: Wheatley DN,Wang AM,Strugnell GE

    更新日期:1996-01-01 00:00:00

  • The significance of alpha 5 beta 1 integrin dependent and independent actin cytoskelton organization in cell transformation and survival.

    abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0964

    authors: Rajeswari J,Pande G

    更新日期:2002-01-01 00:00:00

  • Adult peripheral blood mononuclear cells transdifferentiate in vitro and integrate into the retina in vivo.

    abstract::Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuc...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100146

    authors: Liu Q,Guan L,Huang B,Li W,Su Q,Yu M,Xu X,Luo T,Lin S,Sun X,Chen M,Chen X

    更新日期:2011-06-01 00:00:00

  • Acetylation of alpha-tubilin in different bovine cell types: implications for microtubule dynamics in interphase and mitosis.

    abstract::Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. In the pre...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1006

    authors: Wolf KW,Spanel-Borowski K

    更新日期:1995-01-01 00:00:00

  • Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling.

    abstract::Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11244

    authors: Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

    更新日期:2020-02-01 00:00:00

  • CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.

    abstract::Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis and high mortality. The role of CCN5 has attracted a great focus on the regulation of cancer progression. However, the biological function and mechanism of CCN5 in OSCC are still not well elucidated. The current study was designed to determine ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11296

    authors: Sun S,Cui Z,Yan T,Wu J,Liu Z

    更新日期:2020-04-01 00:00:00

  • ABCC9, NKAPL, and TMEM132C are potential diagnostic and prognostic markers in triple-negative breast cancer.

    abstract::Triple-negative breast cancer (TNBC) is a highly heterogeneous disease. The aim of this study is to identify the diagnostic and poor prognostic signatures in TNBC by exploring the aberrant DNA methylation and gene expression. Differential expression and methylation analysis of the TNBC and paracancer samples from The ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11406

    authors: Zhang X,Kang X,Jin L,Bai J,Zhang H,Liu W,Wang Z

    更新日期:2020-10-01 00:00:00

  • Factors influencing myofibroblast differentiation during wound healing and fibrosis.

    abstract::Granulation tissue fibroblasts (myofibroblasts) develop several ultrastructural and biochemical features of smooth muscle (SM) cells, including the presence of microfilaments bundles and the expression of alpha-SM actin, the actin isoform typical of contractile vascular SM cells. Myofibroblasts have been suggested to ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1006/cbir.1995.1090

    authors: Desmoulière A

    更新日期:1995-05-01 00:00:00

  • The effect of insulin on proteins associated with free, cytoskeletal-bound and membrane-bound polysome populations.

    abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1039

    authors: Moss R,Pryme IF,Vedeler A

    更新日期:1993-12-01 00:00:00

  • Oxidized low-density lipoprotein induces hematopoietic stem cell senescence.

    abstract::We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10121

    authors: Zhang XP,Zhang GH,Wang YY,Liu J,Wei Q,Xu CY,Wang JW,Wang YP

    更新日期:2013-09-01 00:00:00

  • Recombinant PBD-1 (porcine beta-defensin 1) expressed in the milk by transplanting transgenic mES-like-derived cells into mouse mammary gland.

    abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090453

    authors: Huang HJ,Gao QS,Qian YG,Zhang YD,Tao BF,Xiang M,Peng J,Jiang SW,Hause B

    更新日期:2010-10-01 00:00:00

  • Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

    abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10380

    authors: Wan Q,Lu H,Wu LT,Liu X,Xiang JB

    更新日期:2014-12-01 00:00:00

  • Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.

    abstract::Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs cons...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.04.025

    authors: Zhou JM,Xing FY,Shi JJ,Fang ZF,Chen XJ,Chen F

    更新日期:2008-09-01 00:00:00

  • Effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential.

    abstract::An effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential (DeltaPsi(m)) was investigated. Depending on the presence of Mg(2+), addition of Ca(2+) to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate-drive...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.08.024

    authors: Racay P

    更新日期:2008-01-01 00:00:00

  • Tertiary structure of the hepatic cell protein fibrinogen in fluid revealed by atomic force microscopy.

    abstract::Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0216

    authors: Taatjes DJ,Quinn AS,Jenny RJ,Hale P,Bovill EG,McDonagh J

    更新日期:1997-11-01 00:00:00

  • E-cadherin-mediated contact of endothelial progenitor cells with mesenchymal stem cells through β-catenin signaling.

    abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10579

    authors: Xia J,Zhang H,Gao X,Guo J,Hou J,Wang X,Wang S,Yang T,Zhang X,Ge Q,Wan L,Cheng W,Zheng J,Chen X,Wu X

    更新日期:2016-04-01 00:00:00

  • Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5.

    abstract::The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence inclu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10124

    authors: Nie YW,Ding XB,Ge XG,Fan HL,Liu ZW,Guo H

    更新日期:2013-09-01 00:00:00

  • GDNF augments survival and differentiation of TH-positive neurons in neural progenitor cells.

    abstract::GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.01.006

    authors: Sun ZH,Lai YL,Li P,Zuo HC,Xie ZP

    更新日期:2004-01-01 00:00:00

  • Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.

    abstract::Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramm...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10554

    authors: Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J

    更新日期:2016-02-01 00:00:00

  • Fructose sensitizes Jurkat cells oxidative stress-induced apoptosis via caspase-dependent and caspase-independent mechanisms.

    abstract::Whether fructose (FRU), as the sole energy source, confers a metabolic advantage on cancer cells against noxious stimuli is unknown. The aim of this study was to evaluate the effects of low (11 mM), moderate (25 mM), and high (55 mM) FRU concentrations alone or in combination with rotenone (ROT) or doxorubicin (DOX) i...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10653

    authors: Diaz-Aguirre V,Velez-Pardo C,Jimenez-Del-Rio M

    更新日期:2016-11-01 00:00:00

  • Bradykinin receptor-1 activation induces inflammation and increases the permeability of human brain microvascular endothelial cells.

    abstract::Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11232

    authors: Mugisho OO,Robilliard LD,Nicholson LFB,Graham ES,O'Carroll SJ

    更新日期:2019-09-09 00:00:00

  • Constitutive endocytosis in characean internodal cells is independent of an intact actin cytoskeleton.

    abstract::We have investigated constitutive endocytosis in internodal cells of the characean green algae. The endocytic tracer FM1-43 accumulated in distinct plasma membrane domains that are probably enriched in sterol-like substances. Internalization of the dye was active but independent of an intact actin or microtubule cytos...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.11.002

    authors: Foissner I,Klima A

    更新日期:2008-05-01 00:00:00

  • The influence of dose fractionation on radiation-induced haemolysis of human erythrocytes.

    abstract::In this paper the radioresistance of human erythrocytes to radiation-induced haemolysis was studied by means of the split-dose technique. Human erythrocytes suspended in an isotonic Na-phosphatase buffer, pH 7.4 were exposed under air to gamma radiation. Erythrocytes were irradiated with a single dose of 2.7 kGy and w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0188

    authors: Zaborowski A,Szweda-Lewandowska Z

    更新日期:1997-09-01 00:00:00

  • Influence of membrane physical state on lysosomal potassium ion permeability.

    abstract::Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.12.002

    authors: Wang J,Zhang GJ

    更新日期:2005-06-01 00:00:00

  • Different disturbances--one pathway of protein unfolding. Actin folding-unfolding and misfolding.

    abstract::This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellbi.2007.01.025

    authors: Povarova OI,Kuznetsova IM,Turoverov KK

    更新日期:2007-04-01 00:00:00

  • Comparative NMR studies of diffusional water permeability of red blood cells from different species: XVI Dingo (Canis familiaris dingo) and dog (Canis familiaris).

    abstract::As part of a programme of comparative measurements of Pd (diffusional water permeability) the RBCs (red blood cells) from dingo (Canis familiaris dingo) and greyhound dog (Canis familiaris) were studied. The morphologies of the dingo and greyhound RBCs [examined by light and SEM (scanning electron microscopy)] were fo...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090006

    authors: Benga G,Chapman BE,Matei H,Cox GC,Romeo T,Mironescu E,Kuchel PW

    更新日期:2010-03-08 00:00:00

  • Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation.

    abstract::UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and co...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110173

    authors: Ma LL,Meng FB,Shi P,Li G,Pang XN

    更新日期:2012-04-01 00:00:00

  • Uptake of cadmium ions in white-rot fungus Trametes versicolor: effect of cd (II) ions on the activity of laccase.

    abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0887

    authors: Jarosz-Wilkolazka A,Malarczyk E,Pirszel J,Skowroński T,Leonowicz A

    更新日期:2002-01-01 00:00:00

  • Behavior of stem cells under outer-space microgravity and ground-based microgravity simulation.

    abstract::With rapid development of space engineering, research on life sciences in space is being conducted extensively, especially cellular and molecular studies on space medicine. Stem cells, undifferentiated cells that can differentiate into specialized cells, are considered a key resource for regenerative medicine. Researc...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10452

    authors: Zhang C,Li L,Chen J,Wang J

    更新日期:2015-06-01 00:00:00