Inhibition of Ca2+/calmodulin-dependent protein kinase or epidermal growth factor receptor tyrosine kinase abolishes lysophosphatidic acid-mediated DNA-synthesis in human myometrial smooth muscle cells.

Abstract:

:Human myometrial smooth muscle cells (SMCs) were used to evaluate the proliferative activity of lysophosphatidic acid (LPA). This study specifically focuses on the role of Ca(2+)/calmodulin-dependent protein (CaM) kinase and epidermal growth factor (EGF) receptor tyrosine kinase. Myometrial SMCs were cultured from biopsies taken at Cesarean sections. The expression of LPA receptors was determined by reverse transcriptase polymerase chain reaction (RT-PCR), and DNA-synthesis was measured by [3H]thymidine incorporation. LPA(1), LPA(2), and LPA(3)receptor subtypes were detected in the SMCs using RT-PCR. KN-62, an inhibitor of CaM kinase, and Tyrphostin AG 1478, an inhibitor of EGF receptor tyrosine kinase, dose-dependently decreased LPA-stimulated [3H]thymidine incorporation. Furthermore, BB-3103, an inhibitor of matrix metalloproteinases (MMPs), also reduced DNA-synthesis induced by LPA in these cells. The results show, for the first time, that human myometrial SMCs express all three known LPA receptor subtypes. Growth stimulatory effects of LPA on myometrial SMCs seems to be mediated by several pathways, where transactivation of EGF receptors through MMPs appears to be of importance. Furthermore, CaM kinase activity may be critical for LPA signaling since inhibition of CaM kinase totally abolish the proliferative effect of LPA.

journal_name

Cell Biol Int

authors

Nilsson UK,Svensson SP

doi

10.1016/s1065-6995(02)00352-9

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

341-7

issue

4

eissn

1065-6995

issn

1095-8355

pii

S1065699502003529

journal_volume

27

pub_type

杂志文章
  • New indices in morphometry of nuclear structure in the NIH 3T3 cell line.

    abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00158-6

    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Avagyan VY,Karalova EM

    更新日期:2003-01-01 00:00:00

  • Triptolide improves spinal cord injury by promoting autophagy and inhibiting apoptosis.

    abstract::We investigated the effect of triptolide (TP) on spinal cord injury (SCI), and its underlying mechanism. Following the establishment of the SCI model using YFP H-line transgenic mice, TP was intraperitoneally injected at a dose of 0.2 mg/kg once daily for 7 days. Behavioral tests, Nissl staining, and hematoxylin-eosin...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11273

    authors: Zhu N,Ruan J,Yang X,Huang Y,Jiang Y,Wang Y,Cai D,Geng Y,Fang M

    更新日期:2020-03-01 00:00:00

  • RIPK4 suppresses the TGF-β1 signaling pathway in HaCaT cells.

    abstract::Receptor-interacting serine/threonine kinase 4 (RIPK4) and transforming growth factor-β 1 (TGF-β1) play critical roles in the development and maintenance of the epidermis. A negative correlation between the expression patterns of RIPK4 and TGF-β signaling during epidermal homeostasis-related events and suppression of ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11282

    authors: Dinçer T,Boz Er AB,Er İ,Toraman B,Yildiz G,Kalay E

    更新日期:2020-03-01 00:00:00

  • The suppressive effect of CD25+Treg cells on Th1 differentiation requires cell-cell contact partially via TGF-β production.

    abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100528

    authors: Shen E,Zhao K,Wu C,Yang B

    更新日期:2011-07-01 00:00:00

  • Combined application of in vivo UV-crosslinking and in vitro label transfer in the examination of AU-rich sequence binding protein-RNA interactions.

    abstract::A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymph...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1131

    authors: Henics T,Nagy E,Rigby WF

    更新日期:1995-09-01 00:00:00

  • Intranuclear network of 3-5 nm and 8-10 nm fibers in EL-4 lymphoma cells.

    abstract::While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0149

    authors: Padros MR,Giles DM,Suarez MD

    更新日期:1997-06-01 00:00:00

  • Nuclear antigens, as DNA, of DSB389 MAb and some other anti-desmin monoclonal antibodies.

    abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1996.0099

    authors: Kamei H

    更新日期:1996-11-01 00:00:00

  • Erythrocyte sodium/hydrogen exchange inhibition by (-) epicatechin.

    abstract::Epicatechin, a flavonoid belonging to the group of compounds collectively called catechins, have been reported to possess insulin-like properties. Besides their anti-diabetic properties, catechins also show growth inhibition. Since cytosolic pH (pHi) plays a role in cell proliferation and the Na/H exchanger (NHE) is t...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0719

    authors: Matteucci E,Rizvi SI,Giampietro O

    更新日期:2001-01-01 00:00:00

  • Hepatic differentiation of cord blood-derived multipotent progenitor cells (MPCs) in vitro.

    abstract::Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional ne...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.07.017

    authors: Moon YJ,Lee MW,Yoon HH,Yang MS,Jang IK,Lee JE,Kim HE,Eom YW,Park JS,Kim HC,Kim YJ,Lee KH

    更新日期:2008-10-01 00:00:00

  • Wnt-3 and Wnt-3a play different region-specific roles in neural crest development in avians.

    abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090133

    authors: Dongkyun K,Jinsoo S,Jin EJ

    更新日期:2010-07-01 00:00:00

  • Ectopic expression of DAZL gene in goat bone marrow-derived mesenchymal stem cells enhances the trans-differentiation to putative germ cells compared to the exogenous treatment of retinoic acid or bone morphogenetic protein 4 signalling molecules.

    abstract::The plasticity of human and murine bone marrow mesenchymal stem cells (BMSCs) has been proven by their ability to trans-differentiate to multilineage cells, including germ cells. We have investigated ability of goat BMSCs to trans-differentiate to germ cells with extrinsic (e.g., retinoic acid [RA] and BMP4 signalling...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10348

    authors: Yan G,Fan Y,Li P,Zhang Y,Wang F

    更新日期:2015-01-01 00:00:00

  • Aphidicolin inhibits DNA replication in the micronucleus and blocks cytokinesis in Paramecium caudatum.

    abstract::Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0482

    authors: Sabaneyeva E,Tao W,Verbelen JP

    更新日期:1999-01-01 00:00:00

  • Development of transformation vectors based upon a modified plant alpha-tubulin gene as the selectable marker.

    abstract::A plant transformation and selection system has been developed utilizing a modified tubulin gene as a selectable marker. The vector constructs carrying a mutant alpha-tubulin gene from goosegrass conferring resistance to dinitroaniline herbicides were created for transformation of monocotyledonous and dicotyledonous p...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.11.012

    authors: Yemets A,Radchuk V,Bayer O,Bayer G,Pakhomov A,Vance Baird W,Blume YB

    更新日期:2008-05-01 00:00:00

  • KLK14 interactions with HAI-1 and HAI-2 serine protease inhibitors: A molecular dynamics and relative free-energy calculations study.

    abstract::Kallikrein 14 (KLK14) is a serine protease linked to several pathologies including prostate cancer and positively correlates with Gleason score. Though KLK14 functioning in cancer is poorly understood, it has been implicated in HGF/Met signaling, given that KLK14 proteolytically inhibits HGF activator-inhibitor 1 (HAI...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10839

    authors: Solís-Calero C,Carvalho HF

    更新日期:2017-11-01 00:00:00

  • WITHDRAWN: Proteinase activated receptor-2 mediated IL-5, IL-6 and RANTES release from human eosinophils.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.01.011

    authors: Wang H,Huang Y

    更新日期:2008-01-25 00:00:00

  • Functional damage of endothelial progenitor cells is attenuated by 14-3-3-n through inhibition of mitochondrial injury and oxidative stress.

    abstract::Endothelial progenitor cells (EPCs) are precursor cells of vascular endothelial cells, which are widely involved in the pathological process of cardiovascular diseases. EPCs apoptosis could accelerate the process of cardiovascular diseases. 14-3-3-η protein has been proved to be a potent antiapoptosis molecule. Howeve...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11529

    authors: Li Y,Sun X,Zhang X,Zhou H,Wang D,Xia Y,Li X

    更新日期:2020-12-15 00:00:00

  • Dendritic cell uptake of iron-based magnetic nanoparticles.

    abstract::We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no e...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.04.001

    authors: Goya GF,Marcos-Campos I,Fernández-Pacheco R,Sáez B,Godino J,Asín L,Lambea J,Tabuenca P,Mayordomo JI,Larrad L,Ibarra MR,Tres A

    更新日期:2008-08-01 00:00:00

  • Intracellular signal transduction pathways induced by leptin in C2C12 cells.

    abstract::As experimental evidence suggests that leptin may have direct effects on peripheral tissues, we investigated some of the transductional molecules induced by leptin in C2C12 cells. In immunoprecipitation experiments using anti-p85 antibodies (a regulatory subunit of phosphatidylinositol-3-kinase; PI3K), we observed a s...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.03.008

    authors: Maroni P,Bendinelli P,Piccoletti R

    更新日期:2005-07-01 00:00:00

  • ADAMTSL6β promotes fibrillin-1 microfibril assembly, which is possibly mediated via binding through the third thrombospondin type I domain to fibrillin-1.

    abstract::Fibrillin-1 is the major component of extracellular matrix microfibrils. Microfibrils dysfunction is responsible for the onset of various connective tissue diseases, including Marfan syndrome. Although ADAMTSL (a disintegrin and metalloproteinase with thrombospondin motifs-like) 6β is one of the fibrillin-1 binding pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11337

    authors: Orimoto A,Fukuda T

    更新日期:2020-07-01 00:00:00

  • Constitutive endocytosis in characean internodal cells is independent of an intact actin cytoskeleton.

    abstract::We have investigated constitutive endocytosis in internodal cells of the characean green algae. The endocytic tracer FM1-43 accumulated in distinct plasma membrane domains that are probably enriched in sterol-like substances. Internalization of the dye was active but independent of an intact actin or microtubule cytos...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.11.002

    authors: Foissner I,Klima A

    更新日期:2008-05-01 00:00:00

  • Mesenchymal stem cell based therapy for osteo-diseases.

    abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10293

    authors: Ghasroldasht MM,Irfan-Maqsood M,Matin MM,Bidkhori HR,Naderi-Meshkin H,Moradi A,Bahrami AR

    更新日期:2014-10-01 00:00:00

  • Acetylation of alpha-tubilin in different bovine cell types: implications for microtubule dynamics in interphase and mitosis.

    abstract::Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. In the pre...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1006

    authors: Wolf KW,Spanel-Borowski K

    更新日期:1995-01-01 00:00:00

  • Substrate-induced polarisation of cultured epitheliocytes and fibroblasts: non-reactivity of Ras-transformed cells.

    abstract::Non-transformed epitheliocytes and fibroblasts undergo modulations between the less polarised and more polarised phenotypes depending on the nature of the substrate. In contrast, transformed cells express polarised phenotype regardless of the substrate. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0776

    authors: Moizhess TG,Vasiliev JM

    更新日期:2001-01-01 00:00:00

  • Automated detection of morphological alterations during apoptosis.

    abstract::A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell s...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0560

    authors: Lertworasirikul T,Bunyaratvej A,Kubota F

    更新日期:2000-01-01 00:00:00

  • Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.

    abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11437

    authors: Kale VP

    更新日期:2020-11-01 00:00:00

  • Behavior of stem cells under outer-space microgravity and ground-based microgravity simulation.

    abstract::With rapid development of space engineering, research on life sciences in space is being conducted extensively, especially cellular and molecular studies on space medicine. Stem cells, undifferentiated cells that can differentiate into specialized cells, are considered a key resource for regenerative medicine. Researc...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10452

    authors: Zhang C,Li L,Chen J,Wang J

    更新日期:2015-06-01 00:00:00

  • In vitro differentiated hepatic oval-like cells enhance hepatic regeneration in CCl4 -induced hepatic injury.

    abstract::Hepatic oval cells are likely to be activated during advanced stage of liver fibrosis to reconstruct damaged hepatic tissue. However, their scarcity, difficulties in isolation, and in vitro expansion hampered their transplantation in fibrotic liver. This study was aimed to investigate the repair potential of in vitro ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10699

    authors: Awan SJ,Baig MT,Yaqub F,Tayyeb A,Ali G

    更新日期:2017-01-01 00:00:00

  • Decreased intracellular proteolysis correlates with the maintenance of a specific isoenzyme of cytochrome P-450.

    abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0333

    authors: Evans PJ

    更新日期:1999-01-01 00:00:00

  • Upregulation of HO-1 is accompanied by activation of p38MAPK and mTOR in human oesophageal squamous carcinoma cells.

    abstract::Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinom...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10075

    authors: Hu JL,Xiao L,Li ZY,Wang Q,Chang Y,Jin Y

    更新日期:2013-06-01 00:00:00

  • Invariant mRNA and mitotic protein breakdown solves the Russian Doll problem of the cell cycle.

    abstract::It has been proposed that cyclical gene expression occurs at a large number of different times during the cell cycle. The existence of a large number of cycle-specific variations in mRNA and protein during the eukaryotic cell cycle raises the problem of how cell-cycle variations are regulated. This is the "infinite re...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.10.004

    authors: Cooper S,Shedden K,Vu-Phan D

    更新日期:2009-01-01 00:00:00