Abstract:
:CLIC5 (chloride intracellular channel 5) is a CLIC (chloride intracellular channel) with various functions. Its high expression in skeletal muscle and association with actin-based cytoskeleton suggests that it may play an important role in muscle tissue. This study was conducted to examine whether CLIC5 regulates the proliferation and differentiation of C2C12 myoblasts into myotubes. Differentiation of C2C12 myoblasts induced by switching to a differentiation culture medium was accompanied by a significant increase of CLIC5 protein expression level. Constitutive overexpression of CLIC5 was associated with reduced cell proliferation and more cells from G2/M phase into G0/G1 phase, followed by increased number and size of myotubes and up-regulation of muscle-specific proteins of myosin heavy chain, myogenin and desmin. These results demonstrate that CLIC5 is involved in C2C12 proliferation and myogenic differentiation in vitro.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Li F,Yin J,Yue T,Liu L,Zhang Hdoi
10.1042/CBI20090334subject
Has Abstractpub_date
2010-03-08 00:00:00pages
379-84issue
4eissn
1065-6995issn
1095-8355pii
CBI20090334journal_volume
34pub_type
杂志文章abstract::Carpal tunnel syndrome (CTS) is a compression median nerve neuropathy common in women at menopausal age. The aim of this work was to study immunohistochemically the expression of estrogen (ER) and progesterone (PR) receptors in CTS and control specimens. Biopsies of transverse carpal ligament (TCL) and flexor tendon s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.014
更新日期:2008-01-01 00:00:00
abstract::Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this r...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10581
更新日期:2016-05-01 00:00:00
abstract::Ricin is internalized after binding at the cell surface via lectin activity of the B-chain recognizing terminal galactose residues. Ricin-A chain is then translocated to the cytosol from various endocytic structures. Cell death is the result of catalytic inactivation of protein synthesis. Using (125)I-ricin overlays, ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0120
更新日期:1997-03-01 00:00:00
abstract::We previously reported that mechanical vibration-induced proinflammatory cytokines, interleukin-6 (IL-6) and IL-8, expression in human periodontal ligament (hPDL) cells, however, the underlying mechanism remained unclear. Mechanical stimuli are able to activate cellular responses by inducing the activation of several ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11267
更新日期:2020-02-01 00:00:00
abstract::Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1029
更新日期:1994-11-01 00:00:00
abstract::In 3D cultured cell systems, the cells form 3D spheroids that mimic cancer cell spheroids in vivo. Cancer cells form cell spheroids as they grow. The in vivo spheroids do not contain a vascular network; therefore, oxygen and nutrition supplies are insufficient. Specifically, the cells in the core region of the cluster...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10656
更新日期:2016-11-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.10.011
更新日期:2009-02-01 00:00:00
abstract::We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no e...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.001
更新日期:2008-08-01 00:00:00
abstract::Here we report a homologue of the apolipoprotein D gene (AmphiApoD) in amphioxus, Branchiostoma belcheri tsingtauense, the first such finding in a basal chordate cephalochordate. The main features of the protein predicted from AmphiApoD are characteristic of the apolipoprotein D. Phylogenetic analysis places AmphiApoD...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.011
更新日期:2007-01-01 00:00:00
abstract::A number of pathophysiological conditions are related to iron metabolism disturbances. Some of them are well known, others are newly discovered or special. Hepcidin is a newly identified iron metabolism regulating hormone, which could be a promising biomarker for many disorders. In this review, we provide background i...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10505
更新日期:2015-11-01 00:00:00
abstract::G protein-coupled estrogen receptor (GPER) is recently identified as a membrane-associated estrogen receptor that mediates non-genomic effects of estrogen. Our previous immunohistochemistry study found an association between GPER and the proliferation of epithelial ovarian cancer. However, the contributions and mechan...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10243
更新日期:2014-05-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::Using flow cytometry and confocal microscopy, the presence of endorphin, serotonin and chorionic gonadotropin (hCG) was demonstrated in rat white blood cells and peritoneal mast cells. After a single neonatal treatment with beta-endorphin (hormonal imprinting), the mast cells of female rats reaching adulthood containe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00034-9
更新日期:2003-01-01 00:00:00
abstract::We have recently demonstrated that the three principal mammalian isoforms of transforming growth factor beta (TGF-beta) exert distinct effects upon: (1) the migration of confluent adult fibroblasts into 3D gels of native type I collagen fibres (i.e. TGF-beta-1 and -2 had no apparent motogenic activity, whilst TGF-beta...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0423
更新日期:1999-01-01 00:00:00
abstract::Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.002
更新日期:2008-09-01 00:00:00
abstract::Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10776
更新日期:2017-07-01 00:00:00
abstract::The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10006
更新日期:2013-01-01 00:00:00
abstract::Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The intestinal mucosal barrier prevents...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11362
更新日期:2020-08-01 00:00:00
abstract::Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0833
更新日期:2002-01-01 00:00:00
abstract::Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11405
更新日期:2020-10-01 00:00:00
abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11546
更新日期:2021-01-06 00:00:00
abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0898
更新日期:2002-01-01 00:00:00
abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100314
更新日期:2011-02-01 00:00:00
abstract::Rb/E2F regulates many genes that encode proteins required for the cell cycle. Using affymetrix microarrays we previously identified genes regulated by the Rb proteins p130 and p107, many of which are involved in the cell cycle. Several genes with unknown functions were also repressed by p130 and p107, of which some ha...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.013
更新日期:2007-12-01 00:00:00
abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.012
更新日期:2007-02-01 00:00:00
abstract::This review outlines the basic properties of T-tubules in skeletal muscle cells, and the factors that govern reversible vacuolation in T-tubules under experimental conditions. Comparable membranous transformations, involving the plasma membrane or occurring intracellularly, in non-muscle cells are then considered. Fin...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.2002.0945
更新日期:2002-01-01 00:00:00
abstract::Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.009
更新日期:2008-10-01 00:00:00
abstract::The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:
更新日期:1996-04-01 00:00:00
abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11317
更新日期:2020-05-01 00:00:00
abstract::Neuroblastoma is one of the most common solid tumours in children (8-10% of all malignancies). Over 22% of cases have N-myc amplification associated with aggressively growing neuroblastomas. Oncogene-induced sensitization of cells to apoptosis is an important mechanism for suppression of tumorigenesis. Tumour suppress...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110231
更新日期:2012-04-01 00:00:00