Abstract:
:Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-1 fibroblasts. Epithelioid cells survived 3 days at 25 degrees C with unaltered expression of cytokeratin-18 whereas colonies of fibroblasts did not survive more than a day at 25 degrees C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Kaeffer B,Uriel IG,Bottreau Edoi
10.1006/cbir.1994.1029subject
Has Abstractpub_date
1994-11-01 00:00:00pages
1059-65issue
11eissn
1065-6995issn
1095-8355pii
S1065-6995(84)71029-8journal_volume
18pub_type
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