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Intranuclear network of 3-5 nm and 8-10 nm fibers in EL-4 lymphoma cells.

Abstract：

:While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranuclear network in the mouse lymphoma cell line, EL-4. Micrographs of embedment-free sections of extracted cells reveal anastomosing filaments of two different diameters: 3-5 nm and 8-10 nm. The 3-5-nm filaments are interconnected in many junctions and appear to blend smoothly into each other. The 8-10-nm fibers frequently split into two 3-5-nm filaments. Some 3-5-nm fibers appear to be connected at 90 degrees angles with the 8-10-nm fibers. All junctions are smooth with no apparent junction protein. Flow cytometric analysis of RNase A- (and DNase I-) extracted nuclear matrices indicates that they do not contain significant amounts of protein that react with anti-actin and anti-vimentin monoclonal antibodies. Extraction of EL-4 nuclear matrices with high salt does not reveal 8-10-nm core filaments described after similar treatment of tumor cell lines of cervical and mammary origin. The novel characteristics of the core filaments in EL-4 lymphoma cells may reflect cell-type specificity of the nuclear matrix.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Padros MR,Giles DM,Suarez MD

doi

10.1006/cbir.1997.0149

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

367-73

issue

6

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(97)90149-9

journal_volume

21

pub_type

杂志文章

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