Abstract:
:Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell viability, enhanced cell apoptosis, and downregulated P-glycoprotein (P-gp) and multiple drug-resistance protein 1 (MRP1). Moreover, TRIM25 knockdown strengthened the effects of EPI on phosphatase and tensin homolog (PTEN) and phosphorylated (p)-AKT. However, overexpression of TRIM25 exerted an opposite effect, weakening the sensitivity of Huh7 to EPI, and obviously increasing PTEN and reducing p-AKT. Most important, all the changes induced by TRIM25 overexpression in Huh7 were reversed with additional treatment of LY294002 (an AKT pathway inhibitor). Notably, coimmunoprecipitation experiments confirmed the interaction between TRIM25 and PTEN. Knockdown of TRIM25 resulted in reduced ubiquitination of PTEN protein. Collectively, our data suggested that TRIM25 enhanced EPI resistance via modulating PTEN/AKT pathway, and targeting TRIM25 may enhance the sensitivity of HCC cells toward chemotherapy drugs.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Yuan P,Zheng A,Tang Qdoi
10.1002/cbin.11346subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1503-1513issue
7eissn
1065-6995issn
1095-8355journal_volume
44pub_type
杂志文章abstract::Inner ear cells, including hair cells, spiral ganglion cells, stria vascularis cells and supporting cells on the basilar membrane, play a major role in transducing hearing signals and regulating inner ear homoeostasis. However, their functions are often damaged by antibiotic-induced ototoxicity. Apoptosis is probably ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100515
更新日期:2010-12-01 00:00:00
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journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
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journal_title:Cell biology international
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pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10454
更新日期:2015-07-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1109
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0188
更新日期:1997-09-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10397
更新日期:2015-03-01 00:00:00
abstract::Low-temperature stress during microspore development alters cellular organization in rice anthers. The major cellular damage includes unusual starch accumulation in the plastids of the endothecium in postmeiotic anthers, abnormal vacuolation and hypertrophy of the tapetum, premature callose (1,3-beta-glucan) breakdown...
journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2010-03-29 00:00:00
abstract::The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10023
更新日期:2013-02-01 00:00:00
abstract::Human umbilical CD34(+) immature haematopoietic cells were rapidly and efficiently obtained from light density MNC (mononuclear cells) by MACS (magnetic cell sorting). An ex vivo expanded population of CD34(+) was cultured in serum-free medium supplemented with cytokines FL (flt3 ligand), SCF (stem cell factor) and TP...
journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.019
更新日期:2008-09-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章,评审
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更新日期:2017-04-01 00:00:00
abstract::Carpal tunnel syndrome (CTS) is a compression median nerve neuropathy common in women at menopausal age. The aim of this work was to study immunohistochemically the expression of estrogen (ER) and progesterone (PR) receptors in CTS and control specimens. Biopsies of transverse carpal ligament (TCL) and flexor tendon s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.014
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pub_type: 杂志文章,评审
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更新日期:2021-01-02 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.1995.1090
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0039
更新日期:1996-05-01 00:00:00
abstract::As experimental evidence suggests that leptin may have direct effects on peripheral tissues, we investigated some of the transductional molecules induced by leptin in C2C12 cells. In immunoprecipitation experiments using anti-p85 antibodies (a regulatory subunit of phosphatidylinositol-3-kinase; PI3K), we observed a s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.03.008
更新日期:2005-07-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11148
更新日期:2019-07-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11234
更新日期:2019-09-04 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00