Abstract:
:The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition assay indicated that DNR was less cytotoxic for trisomic than for normal cells. Comparison of kinetic parameters and intracellular distribution of this compound showed that the uptake and the amount of intracellular free DNR were greater in normal than in trisomic cells. Contrary to this, there were no significant differences between the amount of DNA-bound drug in both types of cells. TMA-DPH and 12-AS fluorescence anisotropy measurements demonstrated that DNR decreased lipid fluidity in the inner hydrophobic region of plasma membrane in both cell types, but did not influence the fluidity of the outer surface of membrane. We conclude that fibroblasts derived from individuals affected with Down's syndrome are better protected from the damage induced by DNR than normal cells.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Przybylska M,Koceva-Chyla A,Rózga B,Józwiak Zdoi
10.1006/cbir.2000.0583keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
157-70issue
2eissn
1065-6995issn
1095-8355pii
S1065-6995(00)90583-3journal_volume
25pub_type
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