Abstract:
:Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the state of spermatogenesis. HSP27 expression was examined in 30 human testicular biopsy specimens (normal spermatogenesis, maturation arrest and Sertoli cell only syndrome, 10 cases each) using immunofluorescent methods. The biopsies were obtained from patients undergoing investigations for infertility. The seminiferous epithelium of the human testes showing normal spermatogenesis had a cell type-specific expression of HSP27. HSP27 expression was strong in the cytoplasm of the Sertoli cells, spermatogonia, and Leydig cells. Alternatively, the expression was moderate in the spermatocytes, weak in the spermatids and absent in the spermatozoa. In testes showing maturation arrest, HSP27 expression was strong in the Sertoli cells, weak in the spermatogonia, and spermatocytes. It was absent in the spermatids and Leydig cells. In Sertoli cell only syndrome, HSP27 expression was strong in the Sertoli cells and absent in the Leydig cells. We report for the first time the expression patterns of HSP27 in the human testes and show differential expression during normal spermatogenesis, indicating a possible role in this process. The altered expression of this protein in testes showing abnormal spermatogenesis may be related to the pathogenesis of male infertility.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Adly MA,Assaf HA,Hussein MRdoi
10.1016/j.cellbi.2008.07.009subject
Has Abstractpub_date
2008-10-01 00:00:00pages
1247-55issue
10eissn
1065-6995issn
1095-8355pii
S1065-6995(08)00499-Xjournal_volume
32pub_type
杂志文章abstract::Resistance to radiotherapy and chemotherapy has been a major problem of conventional cancer therapies, which consequently leads to cancer relapse and cancer-related death. It is known that cancer stem cells (CSCs) play a key role in therapy resistance and CSC-based targeted therapies have been considered as a powerful...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11456
更新日期:2020-12-01 00:00:00
abstract::TNF-α has long been implicated in the progression of rheumatoid arthritis (RA). However, how the receptors of TNF-α, namely TNFR1 and TNFR2, mediate TNF-α-induced inflammatory responses in fibroblast-like synoviocytes (FLS) in RA has not been elucidated. In the present study, primary FLS cells were isolated from RA pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10735
更新日期:2017-04-01 00:00:00
abstract::The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0765
更新日期:2001-01-01 00:00:00
abstract::It has been reported that chronic inflammation of the vessel wall is a hallmark of atherosclerosis. Interleukin-6 (IL-6) is regarded as an important modulator of inflammatory events occurring during all stages of atherogenesis. Although many factors that induce IL-6 expression have been identified, the effect of homoc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.007
更新日期:2006-07-01 00:00:00
abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0887
更新日期:2002-01-01 00:00:00
abstract::Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0760
更新日期:2001-01-01 00:00:00
abstract::Insulin resistance, diabetes and many kinds of cancers are common in overweight and obese individuals. The tumor suppressor p53 is important in securing genetic stability, but its role in the regulation of metabolic processes and cell differentiation remains unclear. We have investigated the role of p53 in adipocyte d...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10334
更新日期:2014-12-01 00:00:00
abstract::In this study, we determined whether multilineage-differentiating stress-enduring (Muse) cells exist in rat bone marrow and elucidated their effects on protection against the injury of intestinal epithelial cells associated with inflammation. Rat Muse cells were separated from bone marrow mesenchymal stem cells (BMMSC...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11255
更新日期:2020-02-01 00:00:00
abstract::To examine cytokine production in response to RSV infection, we assessed the levels of 29 cytokines released from RSV-infected human foetal lung fibroblasts. We also examined the relationships between the effects of fluticasone propionate and various signalling pathways in the cells. Twenty-four hours after infection ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10044
更新日期:2013-04-01 00:00:00
abstract::Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the under...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10954
更新日期:2018-07-01 00:00:00
abstract::Cytoplasmic proteins with binding capability to AU-rich instability determinant sequences (ARE) of tumour necrosis factor alpha (TNFalpha) mRNA 3' untranslated region (3'UTR) were assessed in human lymphoid cells. In vitro label transfer experiments using wild type as well as mutant sequences in which the 70 nucleotid...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0418
更新日期:1999-01-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.10.011
更新日期:2009-02-01 00:00:00
abstract::MSCs (mesenchymal stem cells) are planned foruse in regenerative medicine to offset age-dependent alterations. However, MSCs are affected by replicative senescence associated with decreasing proliferation potential, telomere shortening and DNA damage during in vitro propagation. To monitor in vitro senescence, we have...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110251
更新日期:2012-01-01 00:00:00
abstract::Transforming growth factor-β (TGF-β) is a potent inhibitor of the growth of normal mammary epithelial cells, and has a pleiotropic, context-dependent, concentration-dependent action. We found attenuation of TGF-β signalling in mammary adenoma carcinoma cells. Phosphorylation at the linker site of Smad2 occurred in a c...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10061
更新日期:2013-05-01 00:00:00
abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1077
更新日期:1993-04-01 00:00:00
abstract::Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10454
更新日期:2015-07-01 00:00:00
abstract::Hypoxia-inducible factor-1α (HIF-1α) and hepatocyte growth factor (HGF) play important roles in postnatal neovascularization. However, the interaction of these two pathways is not fully understood. The present study utilized CoCl(2) treated-endothelial progenitor cells (EPCs) (EPCs exposure to CoCl(2) are under mimic ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10397
更新日期:2015-03-01 00:00:00
abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1039
更新日期:1993-12-01 00:00:00
abstract::Breast cancer is one of the most common cancers in the female population worldwide, and its development is thought to be associated with genetic mutations that lead to uncontrolled and accelerated growth of breast cells. This abnormal behavior requires extra energy, and indeed, tumor cells display a rewired energy met...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11497
更新日期:2020-11-03 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are involved in regulating chromatin modifications, gene transcription, mRNA translation, and protein function. We recently reported a high variation in the basal expression levels of a panel of lncRNAs in HeLa and MCF-7 cells and their differential response to DNA damage induction. Here...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10301
更新日期:2014-09-01 00:00:00
abstract::We have recently demonstrated that the three principal mammalian isoforms of transforming growth factor beta (TGF-beta) exert distinct effects upon: (1) the migration of confluent adult fibroblasts into 3D gels of native type I collagen fibres (i.e. TGF-beta-1 and -2 had no apparent motogenic activity, whilst TGF-beta...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0423
更新日期:1999-01-01 00:00:00
abstract::FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.019
更新日期:2006-12-01 00:00:00
abstract::Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. Programmed death-ligand 1 (PD-L1) is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11139
更新日期:2019-06-01 00:00:00
abstract::We have characterised, using both in vivo and in vitro methods, the effects of the metabotropic glutamate receptor subtype 3 (mGlu3) antagonist (LY341495) and agonist (LY379268) on the proliferation and differentiation of glioma stem cells (GSC). For in vitro studies, a CCK-8 assay was used to determine the cell proli...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10207
更新日期:2014-04-01 00:00:00
abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
更新日期:1994-09-01 00:00:00
abstract::Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0216
更新日期:1997-11-01 00:00:00
abstract::MicroRNA molecules (miRNAs) play important roles in regulating cell behavior. The expression of certain miRNAs has been shown to be regulated by the androgen receptor (AR), which seems to have a critical role in the tumorigenic process of breast cancer. The differential expression of 84 miRNAs was first examined in th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10841
更新日期:2017-12-01 00:00:00
abstract::The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 5'-nucleotidase were assessed by measuring the levels ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.001
更新日期:2006-06-01 00:00:00
abstract::Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10116
更新日期:2013-09-01 00:00:00
abstract::Under the 1G condition, the increase in antipain-sensitive protease activity promptly after UV (mainly 254 nm wavelength) irradiation in cultured human cells is detected and found to be one of the intriguing events involved in suppression of cell mutability. It was found that two cell lines, RSa and its variant UVAP-1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00285-8
更新日期:2003-01-01 00:00:00