Abstract:
:The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216.9 h (N=4) respectively. Proteolysis was substantially decreased at 10 degrees C but the rate of decrease remained constant. Hepatocytes rapidly removed resorufin from the culture medium. The resorufin was not being conjugated or accumulated within the cells. Dicumarol, a potent inhibitor of quinone oxidoreductase, at high concentration (500 microm ) caused only a 72% decrease in the utilization of resorufin. The microsomal detoxifying enzyme, cytochrome P-450 1A1 remained at a constant level in the preserved hepatocyte monolayers. The results of this study strongly favour storing hepatocytes at 10 degrees C rather than at 4 degrees or 37 degrees C.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Evans PJdoi
10.1006/cbir.1998.0333keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
117-24issue
2eissn
1065-6995issn
1095-8355pii
S1065-6995(98)90333-Xjournal_volume
23pub_type
杂志文章abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0343
更新日期:1999-01-01 00:00:00
abstract::Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00157-4
更新日期:2003-01-01 00:00:00
abstract::To better treat patients with non-small cell lung cancer (NSCLC), the investigations on novel molecules affecting NSCLC progression are of vital importance. Long noncoding RNAs (lncRNAs) are identified as pivotal regulators that can affect the cellular activities of carcinomas. Long intergenic non-protein coding RNA 6...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11361
更新日期:2020-08-01 00:00:00
abstract::Stem/progenitor cells hold promise for alleviating/curing type 1 diabetes due to the capacity to differentiate into functional insulin-producing cells. The current study aims to assess the differentiation potential of human pancreatic IPCs (islet-derived progenitor cells). IPCs were derived from four human donors and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100152
更新日期:2011-05-01 00:00:00
abstract::In 3D cultured cell systems, the cells form 3D spheroids that mimic cancer cell spheroids in vivo. Cancer cells form cell spheroids as they grow. The in vivo spheroids do not contain a vascular network; therefore, oxygen and nutrition supplies are insufficient. Specifically, the cells in the core region of the cluster...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10656
更新日期:2016-11-01 00:00:00
abstract::Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. Programmed death-ligand 1 (PD-L1) is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11139
更新日期:2019-06-01 00:00:00
abstract::It has been proposed that cyclical gene expression occurs at a large number of different times during the cell cycle. The existence of a large number of cycle-specific variations in mRNA and protein during the eukaryotic cell cycle raises the problem of how cell-cycle variations are regulated. This is the "infinite re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.10.004
更新日期:2009-01-01 00:00:00
abstract::Restriction Endonucleases (REs) may recognize, cleave and remove DNA from fixed chromatin producing specific chromosome banding patterns. However, the modifications produced in the chromatin fibre are not easy to evaluate and compare. The aim of the present investigation was to visualize differences resulting in the t...
journal_title:Cell biology international
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1006/cbir.1996.0026
更新日期:1996-03-01 00:00:00
abstract::MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vec...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10107
更新日期:2013-08-01 00:00:00
abstract::Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100610
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abstract::Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.12.004
更新日期:2004-01-01 00:00:00
abstract::Life cannot be simply defined in biochemical terms but it is associated with autodynamic behaviour. This fact implies that all aspects of cell biology should be viewed in terms of the resultant temporal features. Theoretical arguments indicate that the dynamic state can be explained only by the existence of periodicit...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.2000.0571
更新日期:2000-01-01 00:00:00
abstract::In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT/enhancer-binding protein (C/EBP) family members and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10415
更新日期:2015-04-01 00:00:00
abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11509
更新日期:2020-11-28 00:00:00
abstract::Reactive oxygen species (ROS) act as signaling molecules to regulate various cell functions. Numerous studies have demonstrated ROS to be essential for the differentiation of adipocytes. Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes in mammalian cells. Prx2 is present in the cytoplasm and cell m...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11417
更新日期:2020-10-01 00:00:00
abstract::The function of the gene gilgamesh (89B9-12) encoding a casein kinase in Drosophila spermatogenesis was studied. The chimeric Gilgamesh-GFP protein in spermatocytes is cortically located. In the polar and apolar spermatocytes, it concentrates at the terminal ends of the fusome, the organelle that passes through the sy...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.02.009
更新日期:2009-05-01 00:00:00
abstract::Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11244
更新日期:2020-02-01 00:00:00
abstract::The vasoactive hydrophilic amine histamine is the most important molecule released by mast cells. However, histamine's role in activating intracellular responses in HGFs (human gingival fibroblasts) has not been evaluated to date. In the present study, we investigated the effect of histamine and of Gram-negative [LPS ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100624
更新日期:2011-10-01 00:00:00
abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0964
更新日期:2002-01-01 00:00:00
abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0898
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abstract::The secretion of interferon-gamma (IFN-gamma) by natural killer (NK) cells following in vitro stimulation with interleukin-2 (IL-2) is inhibited by co-incubation with autologous monocytes at a transcriptional level by more than sixty-fold. In this study, we investigate the nature of the inhibitory signal and particula...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0077
更新日期:1996-09-01 00:00:00
abstract::MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay...
journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
abstract::Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10258
更新日期:2014-06-01 00:00:00
abstract::To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::Phosphoserine has potential effectiveness as a simple substrate in preparing bone replacement materials, which could enhance bone forming ability. However, there is a need to investigate the independent effect of phosphoserine on osteogenic differentiation of human adipose stem cells (hADSCs). hADSCs were cultured in ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10203
更新日期:2014-03-01 00:00:00
abstract::Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs)...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10779
更新日期:2017-08-01 00:00:00
abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0813
更新日期:2001-01-01 00:00:00
abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090133
更新日期:2010-07-01 00:00:00
abstract::Epicatechin, a flavonoid belonging to the group of compounds collectively called catechins, have been reported to possess insulin-like properties. Besides their anti-diabetic properties, catechins also show growth inhibition. Since cytosolic pH (pHi) plays a role in cell proliferation and the Na/H exchanger (NHE) is t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0719
更新日期:2001-01-01 00:00:00
abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11200
更新日期:2019-07-10 00:00:00