Activated macrophages as key mediators of capsule formation on adipose constructs in tissue engineering chamber models.

Abstract:

:In plastic and reconstructive field, it would be much beneficial to fabricate an engineered adipose tissue substitute allowing reliable and complete fat tissue regeneration. Tissue engineering chamber (TEC) holds the promise to optimize an adipogenic configuration that is efficacious as well as reproducible. A frequently occurring complication involves the adipose tissue flap encapsulation and, effectively, its shielding, by a thick fibrous membrane, which hinders development into the proliferative stage. The reason for the deposition of the collagen capsule remains unclear. Numerous studies have highlighted that macrophages play a key role in adipogenesis in a TEC model using a silicone chamber enclosing the fat flap with a superficial epigastric pedicle. As a verification of the role of macrophages in capsule formation, we propose the inhibition of transforming growth factor β1 (TGF-β1) synthesis by macrophage populations in the local microenvironment by administrating tranilast into the TEC. We hypothesize that upon reduction of TGF-β1 levels, capsule formation and inhibition of new adipose tissue development will decrease. Furthermore, we propose that a tissue engineering chamber model in which macrophages are closely related to both neo-adipogenesis and capsule formation.

journal_name

Cell Biol Int

authors

Zhan W,Lu F

doi

10.1002/cbin.10731

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

354-360

issue

4

eissn

1065-6995

issn

1095-8355

journal_volume

41

pub_type

杂志文章,评审
  • Gelatinases and metalloproteinase inhibitor secreted by murine colonic carcinoma cells with differing metastatic potential.

    abstract::Gelatinase activity and inhibitory activity against collagenase were measured in serum-free medium conditioned by murine colonic carcinoma cells with different spontaneous metastatic potentials to the lung. The medium conditioned with poorly metastatic NM11 cells gave higher inhibitory activity than that conditioned w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1057

    authors: Kishi J,Tanaka R,Koiwai O,Yamagata S,Numata Y,Hayakawa T,Shimizu S

    更新日期:1994-03-01 00:00:00

  • High-glucose concentration aggravates TNF-alpha-induced cell viability reduction in human CD146-positive periodontal ligament cells via TNFR-1 gene demethylation.

    abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11445

    authors: Luo H,Zhu W,Mo W,Liang M

    更新日期:2020-12-01 00:00:00

  • Differential sensitivity of human hepatoma cell line and primary rat hepatocyte culture to benzo(a)pyrene-induced unscheduled DNA synthesis and adduct formation.

    abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1083

    authors: Liu TY,Chao TW,Chiang SH,Chi CW

    更新日期:1993-04-01 00:00:00

  • All-trans retinoic acid displays multiple effects on the growth, lipogenesis and adipokine gene expression of AML-I preadipocyte cell line.

    abstract::Adipose tissue is a potential site of retinoic acid (RA) action, but its physiological significance remains to be clarified. We have examined the effect of all-trans retinoic acid (ATRA) on growth and differentiation of preadipocytes, and on adipokine gene expression in mature adipocytes using human preadipocyte cell ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10005

    authors: Morikawa K,Hanada H,Hirota K,Nonaka M,Ikeda C

    更新日期:2013-01-01 00:00:00

  • Borealin is repressed in response to p53/Rb signaling.

    abstract::Rb/E2F regulates many genes that encode proteins required for the cell cycle. Using affymetrix microarrays we previously identified genes regulated by the Rb proteins p130 and p107, many of which are involved in the cell cycle. Several genes with unknown functions were also repressed by p130 and p107, of which some ha...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.06.013

    authors: Date DA,Jacob CJ,Bekier ME,Stiff AC,Jackson MW,Taylor WR

    更新日期:2007-12-01 00:00:00

  • Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.

    abstract::Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramm...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10554

    authors: Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J

    更新日期:2016-02-01 00:00:00

  • Sterol glucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica.

    abstract::Mutants of the methanol-utilizing yeast Pichia pastoris and the alkane-utilizing yeast Yarrowia lipolytica defective in the orthologue of UGT51 (encoding sterol glucosyltransferase) were isolated and compared. These mutants do not contain the specific ergosterol derivate, ergosterol glucoside. We observed that the P. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2003.08.004

    authors: Stasyk OV,Nazarko TY,Stasyk OG,Krasovska OS,Warnecke D,Nicaud JM,Cregg JM,Sibirny AA

    更新日期:2003-01-01 00:00:00

  • Protection against MPP+ neurotoxicity in cerebellar granule cells by antioxidants.

    abstract::The neuropathology associated with Parkinson's disease (PD) is thought to involve excessive production of free radicals, dopamine autoxidation, defects in glutathione peroxidase expression, attenuated levels of reduced glutathione, altered calcium homeostasis, excitotoxicity and genetic defects in mitochondrial comple...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.03.005

    authors: González-Polo RA,Soler G,Rodríguezmartín A,Morán JM,Fuentes JM

    更新日期:2004-01-01 00:00:00

  • Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule.

    abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.09.012

    authors: Sawhney RS

    更新日期:2005-02-01 00:00:00

  • A dynamic ratio of the alpha+ and alpha- isoforms of the tight junction protein ZO-1 is characteristic of Caco-2 cells and correlates with their degree of differentiation.

    abstract::ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090067

    authors: Ciana A,Meier K,Daum N,Gerbes S,Veith M,Lehr CM,Minetti G

    更新日期:2010-05-07 00:00:00

  • Suppression of ATP-induced Cl(-)secretion by enhanced expression of epithelial Na(+)channels in mouse endometrial epithelium.

    abstract::We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0754

    authors: Wang XF,Tsang LL,So SC,Chan HC

    更新日期:2001-01-01 00:00:00

  • WITHDRAWN: Proteinase activated receptor-2 mediated IL-5, IL-6 and RANTES release from human eosinophils.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.01.011

    authors: Wang H,Huang Y

    更新日期:2008-01-25 00:00:00

  • Changes in VEGF expression and DNA synthesis in hepatocytes from hepatectomized and tumour-bearing mice.

    abstract::Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090282

    authors: García MN,Andrini LB,Inda AM,Ronderos JR,Hijano JC,Errecalde AL

    更新日期:2010-02-05 00:00:00

  • Expression patterns of transcription factor PPARγ and C/EBP family members during in vitro adipogenesis of human bone marrow mesenchymal stem cells.

    abstract::In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT/enhancer-binding protein (C/EBP) family members and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10415

    authors: Zhao XY,Chen XY,Zhang ZJ,Kang Y,Liao WM,Yu WH,Xiang AP

    更新日期:2015-04-01 00:00:00

  • Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees.

    abstract::The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090370

    authors: Patricio K,Calábria LK,Peixoto PM,Espindola FS,Da Cruz-Landim C

    更新日期:2010-10-01 00:00:00

  • Hypoxia-induced apoptosis of astrocytes is mediated by reduction of Dicer and activation of caspase-1.

    abstract::Hypoxia is a condition in which the whole body or a region of the body is deprived of oxygen supply. The brain is very sensitive to the lack of oxygen and cerebral hypoxia can rapidly cause severe brain damage. Astrocytes are essential for the survival and function of neurons. Therefore, protecting astrocytes against ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11335

    authors: Kang YW,Kim YS,Park JY,Chu GE,Yang YC,Choi BY,Cho WG

    更新日期:2020-06-01 00:00:00

  • Characterization and expression of amphioxus ApoD gene encoding an archetype of vertebrate ApoD proteins.

    abstract::Here we report a homologue of the apolipoprotein D gene (AmphiApoD) in amphioxus, Branchiostoma belcheri tsingtauense, the first such finding in a basal chordate cephalochordate. The main features of the protein predicted from AmphiApoD are characteristic of the apolipoprotein D. Phylogenetic analysis places AmphiApoD...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.09.011

    authors: Wang L,Zhang S,Liu Z,Li H,Wang Y,Jiang S

    更新日期:2007-01-01 00:00:00

  • Association of nucleus and centrosome: magnet or velcro?

    abstract::A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10016

    authors: Burakov AV,Nadezhdina ES

    更新日期:2013-02-01 00:00:00

  • Tertiary structure of the hepatic cell protein fibrinogen in fluid revealed by atomic force microscopy.

    abstract::Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0216

    authors: Taatjes DJ,Quinn AS,Jenny RJ,Hale P,Bovill EG,McDonagh J

    更新日期:1997-11-01 00:00:00

  • Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

    abstract::The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0265

    authors: Ghosh S,Paweletz N

    更新日期:1998-01-01 00:00:00

  • A novel estrogen receptor GPER mediates proliferation induced by 17β-estradiol and selective GPER agonist G-1 in estrogen receptor α (ERα)-negative ovarian cancer cells.

    abstract::G protein-coupled estrogen receptor (GPER) is recently identified as a membrane-associated estrogen receptor that mediates non-genomic effects of estrogen. Our previous immunohistochemistry study found an association between GPER and the proliferation of epithelial ovarian cancer. However, the contributions and mechan...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10243

    authors: Liu H,Yan Y,Wen H,Jiang X,Cao X,Zhang G,Liu G

    更新日期:2014-05-01 00:00:00

  • Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation.

    abstract::UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and co...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110173

    authors: Ma LL,Meng FB,Shi P,Li G,Pang XN

    更新日期:2012-04-01 00:00:00

  • 3D arrays for high throughput assay of cell migration and electrotaxis.

    abstract::Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10116

    authors: Zhao S,Gao R,Devreotes PN,Mogilner A,Zhao M

    更新日期:2013-09-01 00:00:00

  • Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation.

    abstract::Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of P...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090044

    authors: Huang YC,Yang ZM,Jiang NG,Chen XH,Li XQ,Tan MY,Zhou KP,Tang L,Xie HQ,Deng L

    更新日期:2010-01-27 00:00:00

  • Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line.

    abstract::Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0619

    authors: Cheng SC,Luo D,Xie Y

    更新日期:2001-01-01 00:00:00

  • Tripartite motif protein 25 is associated with epirubicin resistance in hepatocellular carcinoma cells via regulating PTEN/AKT pathway.

    abstract::Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11346

    authors: Yuan P,Zheng A,Tang Q

    更新日期:2020-07-01 00:00:00

  • Intranuclear network of 3-5 nm and 8-10 nm fibers in EL-4 lymphoma cells.

    abstract::While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0149

    authors: Padros MR,Giles DM,Suarez MD

    更新日期:1997-06-01 00:00:00

  • Nuclear deformation characterizes Werner syndrome cells.

    abstract::Mutations in the lamin A gene have been shown, among other defects, to give rise to Hutchinson-Gilford progeria syndrome (HGPS) and to atypical Werner syndrome (WS), both of which are progeroid disorders. Here, we have investigated well-characterized WS patient cell strains that are compound heterozygous for mutations...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.10.011

    authors: Adelfalk C,Scherthan H,Hirsch-Kauffmann M,Schweiger M

    更新日期:2005-12-01 00:00:00

  • Mycobacterial growth in human macrophages: variation according to donor, inoculum and bacterial strain.

    abstract::The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0679

    authors: Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PD

    更新日期:2001-01-01 00:00:00

  • Peroxynitrite activates K+-Cl- cotransport in human erythrocytes.

    abstract::Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0778

    authors: Grzelak A,Mazur J,Bartosz G

    更新日期:2001-01-01 00:00:00