Abstract:
:The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium bovis BCG and M. tuberculosis H37Rv, using a large number of human macrophage donors, (132 and 40, respectively), but no correlation was seen with the TB status of the MDM donor. Clumping of the mycobacteria resulted in more vigorous growth in MDM, suggesting that inoculum size could affect disease progression. The growth rates of 17 clinical isolates of M. tuberculosis were measured in macrophages derived from three donors and no consistent or marked differences between isolates were observed over the 5-day period of growth measurement. However, all 17 clinical strains grew consistently faster than H37Rv in the same experiments.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PDdoi
10.1006/cbir.2000.0679keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
71-81issue
1eissn
1065-6995issn
1095-8355pii
S1065-6995(00)90679-6journal_volume
25pub_type
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