Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees.

Abstract:

:The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.

journal_name

Cell Biol Int

authors

Patricio K,Calábria LK,Peixoto PM,Espindola FS,Da Cruz-Landim C

doi

10.1042/CBI20090370

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1041-7

issue

10

eissn

1065-6995

issn

1095-8355

pii

CBI20090370

journal_volume

34

pub_type

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