Abstract:
:The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Patricio K,Calábria LK,Peixoto PM,Espindola FS,Da Cruz-Landim Cdoi
10.1042/CBI20090370subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1041-7issue
10eissn
1065-6995issn
1095-8355pii
CBI20090370journal_volume
34pub_type
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