Abstract:
:To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary explant epithelial cultures grown on protein-binding membranes. Immunofluorescence staining with specific antibodies indicated that type I and type III collagen were constituents of lens basement membrane. We observed that deposition of type III collagen was more than type I collagen. The synthesis of fibrillar collagen by lens epithelium and its deposition in the lens capsule was established by localization of fibrillar collagen by transmission immunoelectron microscopy. These results demonstrate for the first time that normal lens epithelium synthesize fibrillar collagen which is an intrinsic component of the anterior lens capsule basement membrane.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Sawhney RSdoi
10.1016/j.cellbi.2004.09.012keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
133-7issue
2eissn
1065-6995issn
1095-8355pii
S1065-6995(04)00226-4journal_volume
29pub_type
杂志文章abstract::It has been demonstrated that the crude extract of mosquito larvae alters the mitotic rate of several mouse cell populations of young growing mice (25 +/- 1 days old). Furthermore, the dialysed fraction of the extract inhibited proliferation of hepatocytes from hepatectomized adult male mice (90 days old). Sampling du...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0074
更新日期:1996-08-01 00:00:00
abstract::Insulin-like growth factor-1 (IGF-1) is a positive regulator in proliferation and differentiation of skeletal muscle cells, while myostatin (MSTN) is a member of transforming growth factor beta superfamily that acts as a negative regulator of skeletal muscle mass. The present study was performed to detail whether a co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.05.007
更新日期:2007-10-01 00:00:00
abstract::The plasticity of human and murine bone marrow mesenchymal stem cells (BMSCs) has been proven by their ability to trans-differentiate to multilineage cells, including germ cells. We have investigated ability of goat BMSCs to trans-differentiate to germ cells with extrinsic (e.g., retinoic acid [RA] and BMP4 signalling...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10348
更新日期:2015-01-01 00:00:00
abstract::We have characterised, using both in vivo and in vitro methods, the effects of the metabotropic glutamate receptor subtype 3 (mGlu3) antagonist (LY341495) and agonist (LY379268) on the proliferation and differentiation of glioma stem cells (GSC). For in vitro studies, a CCK-8 assay was used to determine the cell proli...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10207
更新日期:2014-04-01 00:00:00
abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.04.007
更新日期:2004-01-01 00:00:00
abstract::Extracellular nucleotides cause neutrophil degranulation by activating the purinergic receptor subtype P2Y. However, the molecular mechanism involved in the signal pathway remains unknown. A hypothetical scheme suggesting that leukotriene(s) and leukotriene receptor(s) activation is required for extracellular nucleoti...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00082-9
更新日期:2003-01-01 00:00:00
abstract::Stem cell biology has gained remarkable interest in recent years, driven by the hope of finding cures for numerous diseases including skin wound healing through transplantation medicine. Initially upon transplantation, these cells home to and differentiate within the injured tissue into specialised cells. Contrariwise...
journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.03.003
更新日期:2009-12-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090334
更新日期:2010-03-08 00:00:00
abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11469
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abstract::At the end of transcardial perfusions with ice-cold physiological saline for 30 min or with isoosmotic potassium chloride for 5 min, but immediately before perfusion fixation, condenser-discharge electric shocks were administered to rats through surface electrodes pressed onto the temporal muscles of the scalped skull...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.11.002
更新日期:2006-02-01 00:00:00
abstract::Genistein is one of the naturally occurring isoflavones present in plants such as soybeans and is commonly found in a variety of human foods. A number of studies indicated that this class of compounds exerts anticancerogenic and antimutagenic effects in various in vitro systems and in vivo animal models. We studied th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.004
更新日期:2008-08-01 00:00:00
abstract::The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine at concentrations o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0231
更新日期:1998-01-01 00:00:00
abstract::Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.02.006
更新日期:2008-07-01 00:00:00
abstract::The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0265
更新日期:1998-01-01 00:00:00
abstract::A significant decrease in tissue pH or acidosis is a common feature of numerous diseases, including RA (rheumatoid arthritis). Cartilage homoeostasis is profoundly affected by local acidosis in the joints. The diuretic, amiloride, is neuroprotective in models of cerebral ischaemia, a property attributable to the inhib...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110432
更新日期:2012-07-01 00:00:00
abstract::Long non-coding (lncRNA) MALAT1 can be increased by hypoxia or ischemic limbs. Also, downregulation of MALAT1 contributes to reduction of cardiomyocyte apoptosis. However, the functional involvement of MALAT1 in myocardial ischemia-reperfusion (I/R) injury has not been defined. This study investigated the functional i...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10701
更新日期:2017-01-01 00:00:00
abstract::GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.01.006
更新日期:2004-01-01 00:00:00
abstract::Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0619
更新日期:2001-01-01 00:00:00
abstract::Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10310
更新日期:2014-10-01 00:00:00
abstract::Human myometrial smooth muscle cells (SMCs) were used to evaluate the proliferative activity of lysophosphatidic acid (LPA). This study specifically focuses on the role of Ca(2+)/calmodulin-dependent protein (CaM) kinase and epidermal growth factor (EGF) receptor tyrosine kinase. Myometrial SMCs were cultured from bio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00352-9
更新日期:2003-01-01 00:00:00
abstract::We have investigated the effect of visible light on animal cells using light-emitting diodes to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10069
更新日期:2013-06-01 00:00:00
abstract::Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this r...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10581
更新日期:2016-05-01 00:00:00
abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1102
更新日期:1994-07-01 00:00:00
abstract::In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.020
更新日期:2008-10-01 00:00:00
abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.05.008
更新日期:2005-10-01 00:00:00
abstract::Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090063
更新日期:2010-02-22 00:00:00
abstract::Conventional kinesin is an ATPase-active motor protein that is involved in the microtubule-dependent transport of cytoplasmic cargoes. The present paper provides a condensed overview on kinesin-1 and its motility generation outside a living cell. Moreover, it demonstrates that the neuron-specific kinesin KIF5A enables...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.12.001
更新日期:2008-05-01 00:00:00
abstract::In plastic and reconstructive field, it would be much beneficial to fabricate an engineered adipose tissue substitute allowing reliable and complete fat tissue regeneration. Tissue engineering chamber (TEC) holds the promise to optimize an adipogenic configuration that is efficacious as well as reproducible. A frequen...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10731
更新日期:2017-04-01 00:00:00