Expression of glutamyl aminopeptidase by osteogenic induction in rat bone marrow stromal cells.

Abstract:

:Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression Array 230A, Affymetrix Inc., Santa Clara, CA, USA) to demonstrate that GluAP was upregulated in osteogenic induced rat bone marrow stromal cells (BMSCs). To compare the expression of GluAP in the osteogenic differentiation and non-osteogenic differentiation of rat BMSCs in vitro, the cells were osteogenic induced in vitro. We also performed an MTT assay, alkaline phosphatase assay, alizarin red staining, and an immunohistochemical analysis to determine the osteogenic differentiation of BMSCs. The expression of GluAP was examined by real-time polymerase chain reaction (PCR). The real-time PCR results showed that GluAP was upregulated in osteogenic differentiated BMSCs in vitro, suggesting that GluAP may be correlated with the osteogenic differentiation of BMSCs.

journal_name

Cell Biol Int

authors

Wu Y,Xiao J,Wu L,Tian W,Liu L

doi

10.1016/j.cellbi.2008.02.006

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

748-53

issue

7

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(08)00341-7

journal_volume

32

pub_type

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