Abstract:
:Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors. However, its clinical and biological roles in acute myeloid leukemia (AML) remain unclear. This study showed that HOXA-AS2 was overexpressed in AML patients. In addition, the increased HOXA-AS2 expression was correlated with higher white blood cell and bone marrow blast counts, unfavorable karyotype classification, more measurable residual disease positivity, and earlier death. There was also a tendency toward inferior survival in patients with high HOXA-AS2 expression, and HOXA-AS2 was an independent prognostic factor among the normal-karyotype AMLs. Furthermore, the results of in vitro study showed that silencing HOXA-AS2 significantly inhibited the growth of leukemic cells by inducing G1/G0-phase arrest and apoptosis. Further analysis demonstrated that silencing HOXA-AS2 suppressed the phosphorylation level of PI3K and AKT, which thereafter promoted the expression of P21 and P27. Moreover, it was suggested that the sex-determining region Y-box 4 (SOX4), which is closely involved in the PI3K/AKT pathway, might be one of the major downstream targets of HOXA-AS2. Silencing HOXA-AS2 decreased the expression of SOX4, whereas the upregulation of SOX4 partially abrogated the inhibitory effect of silencing HOXA-AS2 on leukemic cells. In conclusion, these findings suggest that HOXA-AS2 probably functions as an oncogene via SOX4/PI3K/AKT pathway and might be a useful biomarker for the prognostic prediction in AML patients, providing a potential therapeutic target for AML.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Qu Y,Wang Y,Wang P,Lin N,Yan X,Li Ydoi
10.1002/cbin.11370subject
Has Abstractpub_date
2020-08-01 00:00:00pages
1745-1759issue
8eissn
1065-6995issn
1095-8355journal_volume
44pub_type
杂志文章abstract::Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Multiv...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10911
更新日期:2018-04-01 00:00:00
abstract::Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs)...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10779
更新日期:2017-08-01 00:00:00
abstract::This review summarizes original and literature data on changes in the ultrastructure of major cell organelles during apoptosis obtained by transmission electron microscopy. Organelles that make the most crucial contribution to the initiation of apoptosis: plasma membrane, mitochondria, proteasomes, Golgi apparatus, an...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11148
更新日期:2019-07-01 00:00:00
abstract::Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0657
更新日期:2001-01-01 00:00:00
abstract::Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11405
更新日期:2020-10-01 00:00:00
abstract::This report deals with observations on the directional locomotion of amoeba before and after fixation and scanning electron microscopy. The study was aimed at visualization of the stepwise events of directional movements. After the analysis of the data it is proposed that the amoeba undergoes a sequence of movement ev...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.01.009
更新日期:2007-08-01 00:00:00
abstract::Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Sev...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0517
更新日期:2000-01-01 00:00:00
abstract::Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00157-4
更新日期:2003-01-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular pathogen, replicating only within a specialized membrane-bounded cytoplasmic vacuole, the parasitophorous vacuole (PV), which interacts with host cell mitochondria. High mobility group box 1 (HMGB1), a known nuclear transcription factor, also may be involved in pathologic...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.031
更新日期:2008-02-01 00:00:00
abstract::We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56(lck), increase in inositol-3-phosphate levels and increase in intracellular calcium concentration...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0837
更新日期:2002-01-01 00:00:00
abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10019
更新日期:2013-02-01 00:00:00
abstract::Emerging evidence suggests that miR-143 plays an important role in the regulation of tumor sensitivity to chemotherapeutic agents. The study explores the underlying mechanism of miR-143 in reversing cisplatin resistance in ovarian cancer. The cisplatin-resistant ovarian cancer cell line A2780/CDDP was induced and esta...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11486
更新日期:2021-01-01 00:00:00
abstract::Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the under...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10954
更新日期:2018-07-01 00:00:00
abstract::Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. Programmed death-ligand 1 (PD-L1) is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11139
更新日期:2019-06-01 00:00:00
abstract::The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and ma...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100919
更新日期:2012-08-01 00:00:00
abstract::The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transport...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.009
更新日期:2009-12-01 00:00:00
abstract::We have characterised, using both in vivo and in vitro methods, the effects of the metabotropic glutamate receptor subtype 3 (mGlu3) antagonist (LY341495) and agonist (LY379268) on the proliferation and differentiation of glioma stem cells (GSC). For in vitro studies, a CCK-8 assay was used to determine the cell proli...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10207
更新日期:2014-04-01 00:00:00
abstract::Mutations in the lamin A gene have been shown, among other defects, to give rise to Hutchinson-Gilford progeria syndrome (HGPS) and to atypical Werner syndrome (WS), both of which are progeroid disorders. Here, we have investigated well-characterized WS patient cell strains that are compound heterozygous for mutations...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.10.011
更新日期:2005-12-01 00:00:00
abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0089
更新日期:1996-10-01 00:00:00
abstract::The action of theophylline on the uptake of alpha-aminoisobutyric acid (AIB; an indicator of anti-inflammatory potency) stimulated by the glucocorticoid, dexamethasone, in cultured rat fibroblast monolayers was evaluated. Theophylline alone (0.1 m m) did not show significant activity (3314+/-27 cpm) compared with the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0309
更新日期:1998-01-01 00:00:00
abstract::Life cannot be simply defined in biochemical terms but it is associated with autodynamic behaviour. This fact implies that all aspects of cell biology should be viewed in terms of the resultant temporal features. Theoretical arguments indicate that the dynamic state can be explained only by the existence of periodicit...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.2000.0571
更新日期:2000-01-01 00:00:00
abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10293
更新日期:2014-10-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) is a highly heterogeneous disease. The aim of this study is to identify the diagnostic and poor prognostic signatures in TNBC by exploring the aberrant DNA methylation and gene expression. Differential expression and methylation analysis of the TNBC and paracancer samples from The ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11406
更新日期:2020-10-01 00:00:00
abstract::EGFP (enhanced green fluorescent protein) tagged to either the N (amino)-terminus [EGFP/hERG (human ether-a-go-go-related gene)] or C (carboxyl)-terminus (hERG/EGFP) of hERG channel is used to study mutant channel protein trafficking for several years. However, it has been reported that the process can alter hERG chan...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100022
更新日期:2011-03-01 00:00:00
abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090453
更新日期:2010-10-01 00:00:00
abstract::Hepatic oval cells are likely to be activated during advanced stage of liver fibrosis to reconstruct damaged hepatic tissue. However, their scarcity, difficulties in isolation, and in vitro expansion hampered their transplantation in fibrotic liver. This study was aimed to investigate the repair potential of in vitro ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10699
更新日期:2017-01-01 00:00:00
abstract::Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090063
更新日期:2010-02-22 00:00:00
abstract::Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated wi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11115
更新日期:2019-05-01 00:00:00
abstract::Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and tr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.11.013
更新日期:2004-01-01 00:00:00
abstract::G protein-coupled estrogen receptor (GPER) is recently identified as a membrane-associated estrogen receptor that mediates non-genomic effects of estrogen. Our previous immunohistochemistry study found an association between GPER and the proliferation of epithelial ovarian cancer. However, the contributions and mechan...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10243
更新日期:2014-05-01 00:00:00