Abstract:
:Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Several non-ribosomal proteins were detected in the polysome preparations by gel electrophoresis and Western blot analysis: (1) a canonical actin of mol wt 42 kDa; (2) a 40 kDa protein, demonstrating affinity for ribosomes, sharing some determinants with actin, and present predominantly in the subpopulations of bound polysomes; and (3) an acidic ribosome-associated p40 evenly distributed between free and bound polysomes. The possibility of involvement of these proteins in interactions between polysomes and the actin cytoskeleton is discussed.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Klyachko N,Aksenova L,Dunaeva M,Kulikova Adoi
10.1006/cbir.1999.0517keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
351-8issue
6eissn
1065-6995issn
1095-8355pii
S1065-6995(99)90517-6journal_volume
24pub_type
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journal_title:Cell biology international
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pub_type: 杂志文章
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journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0323
更新日期:1999-01-01 00:00:00
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journal_title:Cell biology international
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journal_title:Cell biology international
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journal_title:Cell biology international
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journal_title:Cell biology international
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journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
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doi:10.1016/j.cellbi.2008.04.004
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