Extracts from mdx and normal mouse skeletal muscle contain similar levels of mitogenic activity for myoblasts.

Abstract:

:The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compared mitogenic activity for skeletal myoblasts released after injury in mdx and control mice, using crushed muscle extract (CME) as a model system. We found that CMEs from normal and mdx mice contained similar mitogenic activities per microgram protein, and produced similar maximal levels of mitogenic stimulation. Skeletal muscles from mdx mice, however, released higher amounts of CME protein per gram of muscle weight compared to controls, possibly as a result of histological or physiological alterations in mdx muscle tissue. Adequate mitogenic activity in CME from mdx muscles may be related to successful muscle regeneration in mdx mice.

journal_name

Cell Biol Int

authors

Chen G,Bressler BH,Quinn LS

doi

10.1006/cbir.1994.1068

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

229-35

issue

4

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(84)71068-7

journal_volume

18

pub_type

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