Abstract:
:To investigate the role of peroxisome proliferator-activated receptor gamma (PPARgamma) in adipocyte formation within the skeletal muscle of beef cattle, fibroblast-like cells were isolated from the longissimus muscle of cattle and cultured with activators of murine PPARgammaA thiazolidinedione T-174, which is a specific ligand for PPARgamma, stimulated adipose differentiation (evaluated by counting differentiated adipocytes under microscopic observation) in a dose-dependent fashion. A peroxisome proliferator Wy14,643 which strongly activates the alpha isoform of murine PPAR also stimulated differentiation but its potency was weaker than that of T-174. Unexpectedly, 15-deoxy-Delta12,14-prostaglandin J2, which is believed to be an endogenous ligand for PPARgamma, could not induce adipose differentiation in doses which have been found to be effective on rodent cells. Immunoblotting analysis confirmed the significant expression of PPARgamma protein in fibroblast-like cell cultures prepared from bovine skeletal muscle. In conclusion, bovine skeletal muscle contains adipose precursor cells expressing functionally active PPARgamma.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Torii SI,Kawada T,Matsuda K,Matsui T,Ishihara T,Yano Hdoi
10.1006/cbir.1998.0270keywords:
subject
Has Abstractpub_date
1998-01-01 00:00:00pages
421-7issue
6eissn
1065-6995issn
1095-8355pii
S1065-6995(98)90270-0journal_volume
22pub_type
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