Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model.


:Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this paper, we evaluated for the first time the expression of klb mRNA during early development of Xenopus laevis by RT-PCR and whole mount in situ hybridization. RT-PCR experiments showed that the expression of klb was initially detected at late gastrula stage followed by a quick increasing and continued expression throughout embryonic development. Whole mount in situ hybridization detected specific expression of klb in many primordial organs at tailbud stage such as liver primordium and pancreatic buds, implying that the hormonal FGF signaling may play a role in the foregut development. The dynamic and specific expression patterns of klb also suggest that Xenopus laevis can serve a convenient model for the function of the hormonal FGF signaling in organogenesis and metabolism regulation during embryonic development.


Cell Biol Int


Chen G,Tao Q




Has Abstract


2019-02-01 00:00:00












  • Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

    abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Wan Q,Lu H,Wu LT,Liu X,Xiang JB

    更新日期:2014-12-01 00:00:00

  • CD14-dependent activation of NF-kappaB by filarial parasitic sheath proteins.

    abstract::Tropical pulmonary eosinophilia is in part caused by the hyperimmune responsiveness of the lung tissue against the antigens of degenerating microfilariae. We have previously shown that the activation of the transcription factor NF-kappaB is essential for the synthesis and release of multiple pro-inflammatory cytokines...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Narayanan K,Seufzer BJ,Brockman-Schneider RA,Gern JE,Balakrishnan A,Miyamoto S

    更新日期:2002-01-01 00:00:00

  • CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.

    abstract::Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis and high mortality. The role of CCN5 has attracted a great focus on the regulation of cancer progression. However, the biological function and mechanism of CCN5 in OSCC are still not well elucidated. The current study was designed to determine ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Sun S,Cui Z,Yan T,Wu J,Liu Z

    更新日期:2020-04-01 00:00:00

  • Low-dose aspirin promotes endothelial progenitor cell migration and adhesion and prevents senescence.

    abstract::Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hu Z,Zhang F,Yang Z,Zhang J,Zhang D,Yang N,Zhang Y,Cao K

    更新日期:2008-07-01 00:00:00

  • PCNA Ki-67 dissociation in childhood acute lymphoblastic leukaemia. An immunofluorescent laser confocal scanning microscopical study.

    abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ball LM,Pope J,Howard CV,Eccles P,van Velzen D

    更新日期:1994-09-01 00:00:00

  • Unique membrane-interacting properties of the immunostimulatory cationic peptide KLKL(5)KLK (KLK).

    abstract::We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation....

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Aichinger MC,Ortbauer M,Reipert S,Zauner W,Bogner P,Froschauer E,Nowikovsky K,Lingnau K,von Gabain A,Schweyen R,Henics T

    更新日期:2008-11-01 00:00:00

  • Enrichment and characterization of mouse putative epidermal stem cells.

    abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EK

    更新日期:2004-01-01 00:00:00

  • Hepatic differentiation of cord blood-derived multipotent progenitor cells (MPCs) in vitro.

    abstract::Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional ne...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Moon YJ,Lee MW,Yoon HH,Yang MS,Jang IK,Lee JE,Kim HE,Eom YW,Park JS,Kim HC,Kim YJ,Lee KH

    更新日期:2008-10-01 00:00:00

  • Oxidized low-density lipoprotein induces hematopoietic stem cell senescence.

    abstract::We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-f...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhang XP,Zhang GH,Wang YY,Liu J,Wei Q,Xu CY,Wang JW,Wang YP

    更新日期:2013-09-01 00:00:00

  • Effect of monochromatic visible light on intracellular superoxide anion production and mitochondrial membrane potential of B16F1 and B16F10 murine melanoma cells.

    abstract::We have investigated the effect of visible light on animal cells using light-emitting diodes to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Sato K,Minai Y,Watanabe H

    更新日期:2013-06-01 00:00:00

  • Role of allograft inflammatory factor-1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells.

    abstract::Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated wi...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou Y,Li X,Yuan X,Hao L

    更新日期:2019-05-01 00:00:00

  • Role of p53 in preadipocyte differentiation.

    abstract::Insulin resistance, diabetes and many kinds of cancers are common in overweight and obese individuals. The tumor suppressor p53 is important in securing genetic stability, but its role in the regulation of metabolic processes and cell differentiation remains unclear. We have investigated the role of p53 in adipocyte d...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Huang Q,Liu M,Du X,Zhang R,Xue Y,Zhang Y,Zhu W,Li D,Zhao A,Liu Y

    更新日期:2014-12-01 00:00:00

  • The effect of oxidative stress induced by t-butyl hydroperoxide on the structural dynamics of membrane proteins of Chinese hamster fibroblasts.

    abstract::A method based on the measurement at room temperature of tryptophan phosphorescence (RTTP) gives the unique possibility to investigate the dynamic structure of membrane proteins without their isolation from cells. This method was used to study the influence of tert- butyl hydroperoxide (t -BHP) on Chinese hamster fibr...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Mazhul V,Shcherbin D,Zavodnik I,Rekawiecka K,Bryszewska M

    更新日期:1999-01-01 00:00:00

  • Chromatin fibers: from classical descriptions to modern interpretation.

    abstract::The first description of intrachromosomal fibers was made by Baranetzky in 1880. Since that time, a plethora of fibrillar substructures have been described inside the mitotic chromosomes, and published data indicate that chromosomes may be formed as a result of the hierarchical folding of chromatin fibers. In this rev...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Kuznetsova MA,Sheval EV

    更新日期:2016-11-01 00:00:00

  • Modification of the alkaline comet assay with human mesenchymal stem cells.

    abstract::MSCs (mesenchymal stem cells) are planned foruse in regenerative medicine to offset age-dependent alterations. However, MSCs are affected by replicative senescence associated with decreasing proliferation potential, telomere shortening and DNA damage during in vitro propagation. To monitor in vitro senescence, we have...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Fuchs R,Stelzer I,Drees CM,Rehnolt C,Schraml E,Sadjak A,Schwinger W

    更新日期:2012-01-01 00:00:00

  • Characterization of a novel murine preadipocyte line, AP-18, isolated from subcutaneous tissue: analysis of adipocyte-related gene expressions.

    abstract::Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Chen C,Takahashi K,Yoshida A,Takizawa Y,Lee Y,Nakui M,Doi H,Takebayashi Y,Fukumoto M,Yamada T,Katagiri H,Oka Y,Satoh J

    更新日期:2010-02-22 00:00:00

  • Phallacidin stains the mitotic spindle of the diatom Pinnularia spp.

    abstract::Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Sampson K

    更新日期:2004-01-01 00:00:00

  • Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.

    abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kale VP

    更新日期:2020-11-01 00:00:00

  • Human endometrial stem cells differentiation into functional hepatocyte-like cells.

    abstract::In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purificatio...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Khademi F,Soleimani M,Verdi J,Tavangar SM,Sadroddiny E,Massumi M,Ai J

    更新日期:2014-07-01 00:00:00

  • Retinoic acid induces myoblasts transdifferentiation into premeiotic Stra8-positive cells.

    abstract::Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Jia W,Cheng D,Chen S,Lei L,Wang H

    更新日期:2011-04-01 00:00:00

  • Intranuclear network of 3-5 nm and 8-10 nm fibers in EL-4 lymphoma cells.

    abstract::While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Padros MR,Giles DM,Suarez MD

    更新日期:1997-06-01 00:00:00

  • Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.

    abstract::The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Yamamoto M,Aoyagi M,Akazawa K,Tajima S,Yamamoto K

    更新日期:1998-01-01 00:00:00

  • Alternatively spliced forms of the P180 ribosome receptor differ in their ability to induce the proliferation of rough endoplasmic reticulum.

    abstract::Expression of the canine 180-kDa ribosome receptor p180 in yeast induces the synthesis of RER, and increases the mRNAs of secretory pathway proteins, and protein secretion. To assess whether p180 is a master regulator of cell secretion in mammalian cells, we stably expressed red fluorescent forms of the human p180 var...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Bai JZ,Leung E,Holloway H,Krissansen GW

    更新日期:2008-05-01 00:00:00

  • Vascular endothelial cells and smooth muscle cells mediate carbachol-induced hepatocyte proliferation via muscarinic receptors and IP3/PKC signaling cascades.

    abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Yoshimura R,Arai J,Endo Y

    更新日期:2009-04-01 00:00:00

  • Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.

    abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Elinos-Báez CM,León F,Santos E

    更新日期:2005-08-01 00:00:00

  • Effects of small interfering RNAs targeting MAPK1 on gene expression profile in HeLa cells as revealed by microarray analysis.

    abstract::The mitogen activated protein kinases (MAPK) signaling cascade plays an important role in cell life. We proved that small interfering RNAs targeting MAPK1 (siRNA-2) could inhibit HeLa cell growth, but the effects of siRNA-2 on gene expression profile were unclear. Using Affymetrix GeneChip HG-U133A 2.0, we identified ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Huang C,Liu LY,Li ZF,Wang P,Ni L,Song LP,Xu DH,Song TS

    更新日期:2008-09-01 00:00:00

  • Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts.

    abstract::We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comp...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Gai H,Leung EL,Costantino PD,Aguila JR,Nguyen DM,Fink LM,Ward DC,Ma Y

    更新日期:2009-11-01 00:00:00

  • Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.

    abstract::Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs cons...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou JM,Xing FY,Shi JJ,Fang ZF,Chen XJ,Chen F

    更新日期:2008-09-01 00:00:00

  • Effects of spaceflight conditions on fertilization and embryogenesis in the sea urchin Lytechinus pictus.

    abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Schatten H,Chakrabarti A,Taylor M,Sommer L,Levine H,Anderson K,Runco M,Kemp R

    更新日期:1999-01-01 00:00:00

  • Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

    abstract::The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ghosh S,Paweletz N

    更新日期:1998-01-01 00:00:00