Propofol induces apoptosis of hepatocellular carcinoma cells by upregulation of microRNA-199a expression.

Abstract:

:Propofol is one of the extensively commonly used intravenous anaesthetic agents. The effects of Propofol on hepatocellular carcinoma (HCC) growth inhibition and apoptosis have been examined. The techniques used were the MTT assay, flow cytometry, real-time PCR to assess miR-199a expression, as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol. Finally, we evaluated the effect of miR-199a on Propofol-induced anti-tumour activity using anti-miR-199a. Propofol efficiently inhibited the growth of HCC cells, but was less toxic to normal hepatic cells. It induced apoptosis and increased expression of miR-199a. Activation of caspase-8 and caspase-9 suggested that both extrinsic and intrinsic pathways are involved in Propofol-induced apoptosis. Anti-miR-199a reversed the effect of Propofol on apoptosis and activation of caspase-8 and caspase-9 in HepG2 cells. Propofol can effectively induce apoptosis of HCC cells and modulation of miR-199a possibly contributes to the anti-tumour action of Propofol. Hence, Propofol might be an effective drug for HCC.

journal_name

Cell Biol Int

authors

Zhang J,Wu GQ,Zhang Y,Feng ZY,Zhu SM

doi

10.1002/cbin.10034

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

227-32

issue

3

eissn

1065-6995

issn

1095-8355

journal_volume

37

pub_type

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