Abstract:
:Acylase 1 was investigated at the cellular level in the rat small intestine along the enterocyte-differentiation axis. As confirmed by microscopic analysis, villus tip cells and crypt cells of rat jejunal mucosa were successfully separated using the Weiser method. The proliferating undifferentiated crypt cells showed much higher ACY 1 activity levels than the villus cells, with a 6.4-fold decrease as the cells migrated and differentiated along the crypt-villus axis. RT-PCR studies on mRNA extracted from isolated cells showed that ACY 1 mRNA was mainly expressed in crypt cells, reaching levels that were 12-fold higher than those recorded in other cell types along the whole enterocyte differentiation axis. It was concluded that the expression of ACY 1 in the intestinal crypt cells is regulated at the mRNA level. Immunohistochemistry revealed the expression of ACY 1 in the absorbing lineage cells from the ileal and colonic crypts and the absence of ACY 1 in the mucus producing goblet cells. These findings proposed ACY 1 as a new marker transcript for absorbing cells of intestinal crypt, which can be used to monitor the process of intestinal N-alpha-acetylated protein metabolism.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Cigna N,Nicoletti C,Durand A,Chaix JC,Giardina T,Perrier Jdoi
10.1016/j.cellbi.2007.03.006subject
Has Abstractpub_date
2007-09-01 00:00:00pages
966-73issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(07)00076-5journal_volume
31pub_type
杂志文章abstract::Low-temperature stress during microspore development alters cellular organization in rice anthers. The major cellular damage includes unusual starch accumulation in the plastids of the endothecium in postmeiotic anthers, abnormal vacuolation and hypertrophy of the tapetum, premature callose (1,3-beta-glucan) breakdown...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090417
更新日期:2010-03-29 00:00:00
abstract::While Bcl-2 protein is involved in the regulation of apoptosis, recent research showed that Beclin1, described as the essential autophagy effector and haploinsufficient tumor suppressor, was originally isolated as a Bcl-2 interacting protein. Beclin1 interacts with Bcl-2 through a BH3 domain; nevertheless, the functio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10333
更新日期:2014-10-01 00:00:00
abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10019
更新日期:2013-02-01 00:00:00
abstract::With rapid development of space engineering, research on life sciences in space is being conducted extensively, especially cellular and molecular studies on space medicine. Stem cells, undifferentiated cells that can differentiate into specialized cells, are considered a key resource for regenerative medicine. Researc...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10452
更新日期:2015-06-01 00:00:00
abstract::Osteocalcin is one of the most abundant non-collagenous proteins in bone that is commonly used as a preliminary biomarker in bone formation. Osteocalcin also regulates energy metabolism as a hormone. Estrogen-related receptor alpha (ERRα) is primarily thought to regulate energy homeostasis through interacting with per...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10148
更新日期:2013-11-01 00:00:00
abstract::As experimental evidence suggests that leptin may have direct effects on peripheral tissues, we investigated some of the transductional molecules induced by leptin in C2C12 cells. In immunoprecipitation experiments using anti-p85 antibodies (a regulatory subunit of phosphatidylinositol-3-kinase; PI3K), we observed a s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.03.008
更新日期:2005-07-01 00:00:00
abstract::In this paper the radioresistance of human erythrocytes to radiation-induced haemolysis was studied by means of the split-dose technique. Human erythrocytes suspended in an isotonic Na-phosphatase buffer, pH 7.4 were exposed under air to gamma radiation. Erythrocytes were irradiated with a single dose of 2.7 kGy and w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0188
更新日期:1997-09-01 00:00:00
abstract::Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0956
更新日期:2002-01-01 00:00:00
abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.05.008
更新日期:2005-10-01 00:00:00
abstract::Advances in dental pulp stem cell (DPSC) biology and behaviour have promised much in the field of regenerative medicine. Their recent use in clinical trials for bone repair enforces the notion that DPSCs can be used successfully in patients; however they display diverse characteristics under different culture conditio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10021
更新日期:2013-02-01 00:00:00
abstract::Using flow cytometry and confocal microscopy, the presence of endorphin, serotonin and chorionic gonadotropin (hCG) was demonstrated in rat white blood cells and peritoneal mast cells. After a single neonatal treatment with beta-endorphin (hormonal imprinting), the mast cells of female rats reaching adulthood containe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00034-9
更新日期:2003-01-01 00:00:00
abstract::Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facin...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100927
更新日期:2011-10-01 00:00:00
abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11317
更新日期:2020-05-01 00:00:00
abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10360
更新日期:2015-02-01 00:00:00
abstract::Ricin is internalized after binding at the cell surface via lectin activity of the B-chain recognizing terminal galactose residues. Ricin-A chain is then translocated to the cytosol from various endocytic structures. Cell death is the result of catalytic inactivation of protein synthesis. Using (125)I-ricin overlays, ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0120
更新日期:1997-03-01 00:00:00
abstract::Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prot...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.03.001
更新日期:2007-09-01 00:00:00
abstract::Tropical pulmonary eosinophilia is in part caused by the hyperimmune responsiveness of the lung tissue against the antigens of degenerating microfilariae. We have previously shown that the activation of the transcription factor NF-kappaB is essential for the synthesis and release of multiple pro-inflammatory cytokines...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0828
更新日期:2002-01-01 00:00:00
abstract::Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.009
更新日期:2008-10-01 00:00:00
abstract::Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10258
更新日期:2014-06-01 00:00:00
abstract::Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5'UTR sequence of some rice beta-tubulin genes can...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.11.013
更新日期:2008-05-01 00:00:00
abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1039
更新日期:1993-12-01 00:00:00
abstract::Conventional kinesin is an ATPase-active motor protein that is involved in the microtubule-dependent transport of cytoplasmic cargoes. The present paper provides a condensed overview on kinesin-1 and its motility generation outside a living cell. Moreover, it demonstrates that the neuron-specific kinesin KIF5A enables...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.12.001
更新日期:2008-05-01 00:00:00
abstract::Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0833
更新日期:2002-01-01 00:00:00
abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0887
更新日期:2002-01-01 00:00:00
abstract::The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10900
更新日期:2018-07-01 00:00:00
abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.012
更新日期:2005-02-01 00:00:00
abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10427
更新日期:2015-05-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular pathogen, replicating only within a specialized membrane-bounded cytoplasmic vacuole, the parasitophorous vacuole (PV), which interacts with host cell mitochondria. High mobility group box 1 (HMGB1), a known nuclear transcription factor, also may be involved in pathologic...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.031
更新日期:2008-02-01 00:00:00
abstract::The objective of this study was to test the hypothesis that cytoskeletal actin fragmentation is mediated through caspase-2, specifically examining the ability of a caspase-2 inhibitor to interfere with actin fragmentation, in comparison with a caspase-3 inhibitor. Cardiomyocytes were cultured from embryonic chick hear...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.07.012
更新日期:2004-01-01 00:00:00
abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0089
更新日期:1996-10-01 00:00:00