Abstract:
:This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl3 treatment inhibited both cell viability and colony forming ability in PC3 cells more significantly than that in DU145 cells. Annexin/PI (propidium iodide) staining showed an increase in apoptotic death of PC3 cells in the presence of GdCl3. Wound healing and adhesion assay indicated that GdCl3 suppressed PC3 cell migration. Western-blot analysis demonstrated that GdCl3 treatment inhibited phosphorylation of ERK (extracellular-signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase). Pretreatment with PTx (pertussis toxin), a Gi protein inhibitor, conferred resistance to GdCl3-induced colony formation, ERK and p38 phosphorylation in PC3 cells. Moreover, GdCl3 inhibited PC3 cell-induced osteoclast differentiation. RT-PCR (reverse transcription-PCR) indicated that GdCl3 decreased the expression of RANKL (receptor activator of nuclear factor-κB ligand) in PC3 cells, whereas it increased the expression of OPG (osteoprotegerin) in PC3 and DU145 cells. In conclusion, the present study indicated that GdCl3 inhibited PC3 cell migration mediated by the inactivation of both ERK and p38 MAPK pathways via PTx-sensitive G proteins, and also suppressed PC3 cell-induced osteoclast differentiation via regulating the mRNA expression of OPG and RANKL.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Wang P,Zou XM,Huang J,Zhang TL,Wang Kdoi
10.1042/CBI20100870subject
Has Abstractpub_date
2011-11-01 00:00:00pages
1159-67issue
11eissn
1065-6995issn
1095-8355pii
CBI20100870journal_volume
35pub_type
杂志文章abstract::The vasoactive hydrophilic amine histamine is the most important molecule released by mast cells. However, histamine's role in activating intracellular responses in HGFs (human gingival fibroblasts) has not been evaluated to date. In the present study, we investigated the effect of histamine and of Gram-negative [LPS ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100624
更新日期:2011-10-01 00:00:00
abstract::Reactive oxygen species (ROS) act as signaling molecules to regulate various cell functions. Numerous studies have demonstrated ROS to be essential for the differentiation of adipocytes. Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes in mammalian cells. Prx2 is present in the cytoplasm and cell m...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11417
更新日期:2020-10-01 00:00:00
abstract::A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0917
更新日期:2002-01-01 00:00:00
abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110183
更新日期:2012-08-01 00:00:00
abstract::Transforming growth factor-β (TGF-β) is a potent inhibitor of the growth of normal mammary epithelial cells, and has a pleiotropic, context-dependent, concentration-dependent action. We found attenuation of TGF-β signalling in mammary adenoma carcinoma cells. Phosphorylation at the linker site of Smad2 occurred in a c...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10061
更新日期:2013-05-01 00:00:00
abstract::Lead is a heavy metal of considerable environmental and occupational concern and there is growing evidence that it is toxic to the human immune system. In this regard, this study examined the effect of lead (Pb) exposure to peritoneal macrophages (Mvarphis) of mice (Mus musculus) cultivated in DMEM medium supplemented...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.010
更新日期:2006-07-01 00:00:00
abstract::Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11241
更新日期:2020-02-01 00:00:00
abstract::Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 degrees C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0247
更新日期:1999-01-01 00:00:00
abstract::MicroRNA molecules (miRNAs) play important roles in regulating cell behavior. The expression of certain miRNAs has been shown to be regulated by the androgen receptor (AR), which seems to have a critical role in the tumorigenic process of breast cancer. The differential expression of 84 miRNAs was first examined in th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10841
更新日期:2017-12-01 00:00:00
abstract::BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchymal stem cell) in the treatment of lung diseases remains controversial. We have asses...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110447
更新日期:2012-06-01 00:00:00
abstract::Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. In the pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1006
更新日期:1995-01-01 00:00:00
abstract::Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.015
更新日期:2009-09-01 00:00:00
abstract::Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11219
更新日期:2019-08-23 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular pathogen, replicating only within a specialized membrane-bounded cytoplasmic vacuole, the parasitophorous vacuole (PV), which interacts with host cell mitochondria. High mobility group box 1 (HMGB1), a known nuclear transcription factor, also may be involved in pathologic...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.031
更新日期:2008-02-01 00:00:00
abstract::Following culture of human peripheral blood mononuclear cells (PBMNC) from 25 normal donors and 15 patients with common variable immunodeficiency (CVID), we were unable to identify any IL10-defective patients. Clear-cut effects of IL4 could be demonstrated in controls, while in CVID all effects are less pronounced. Wh...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1030
更新日期:1994-11-01 00:00:00
abstract::Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs cons...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.025
更新日期:2008-09-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.04.007
更新日期:2004-01-01 00:00:00
abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110322
更新日期:2012-05-01 00:00:00
abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1039
更新日期:1993-12-01 00:00:00
abstract::In 3D cultured cell systems, the cells form 3D spheroids that mimic cancer cell spheroids in vivo. Cancer cells form cell spheroids as they grow. The in vivo spheroids do not contain a vascular network; therefore, oxygen and nutrition supplies are insufficient. Specifically, the cells in the core region of the cluster...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10656
更新日期:2016-11-01 00:00:00
abstract::Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellu...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00125-2
更新日期:2003-01-01 00:00:00
abstract::This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.010
更新日期:2008-06-01 00:00:00
abstract::To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [75 PDL (population doubli...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110460
更新日期:2012-06-01 00:00:00
abstract::Forms of the protozoan of the Hepatozoon genus are detected free in the circulation and also within some of the erythrocytes of infected snakes. In healthy snakes, DNA fragmentation and cell death usually affect a few circulating erythrocytes in agreement with the long life span expected for these cells. In the presen...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.026
更新日期:2007-05-01 00:00:00
abstract::Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1029
更新日期:1994-11-01 00:00:00
abstract::Advances in dental pulp stem cell (DPSC) biology and behaviour have promised much in the field of regenerative medicine. Their recent use in clinical trials for bone repair enforces the notion that DPSCs can be used successfully in patients; however they display diverse characteristics under different culture conditio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10021
更新日期:2013-02-01 00:00:00
abstract::MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vec...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10107
更新日期:2013-08-01 00:00:00
abstract::In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.020
更新日期:2008-10-01 00:00:00
abstract::The spatial distribution of acid membrane organelles and their relationships with normal and vacuolated transverse tubules has been studied in living frog skeletal muscle fibres using confocal microscopy. Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.017
更新日期:2006-11-01 00:00:00