Abstract：
:Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related to checkpoint control and oxidative metabolism. In sublethal concentrations of DNA damaging agents, aph1Δ mutants grew with a substantially shorter lag phase. In aph1Δ mutants carrying a hypomorphic allele of cds1 (the fission yeast homolog of Chk2), in addition, increased chromosome fragmentation and missegregation were found. We also found that under hypoxia or impaired electron transport function, the Aph1 protein level was strongly depressed. Previously, FHIT has been linked to regulation of the human 9-1-1 checkpoint complex constituted by Hus1, Rad1, and Rad9. In Schizosaccharomyces pombe, the levels of all three 9-1-1 proteins are all downregulated by hypoxia in similarity with Aph1. Moreover, deletion of the aph1+ gene reduced the Rad1 protein level, indicating a direct relationship between these two proteins. We conclude that the fission yeast FHIT homolog has a role in modulating DNA damage checkpoint function, possibly through an effect on the 9-1-1 complex, and that this effect may be critical under conditions of limiting oxidative metabolism and reoxygenation.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Sjölander JJ,Sunnerhagen Pdoi
10.1002/cbin.11241subject
Has Abstractpub_date
2020-02-01 00:00:00pages
412-423issue
2eissn
1065-6995issn
1095-8355journal_volume
44pub_type
杂志文章abstract：:Pig endothelial cells are the first cells to interact with human immune components after organ xenotransplantation, which is a procedure currently considered to be the best treatment option for end-stage organ failure. It is, therefore, essential to study the mechanisms of molecular interaction between pig endothelial...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.03.016
更新日期：2005-08-01 00:00:00
abstract：:Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.12.002
更新日期：2005-06-01 00:00:00
abstract：:GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.01.006
更新日期：2004-01-01 00:00:00
abstract：:Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: ma...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11280
更新日期：2020-03-01 00:00:00
abstract：:We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2001.0754
更新日期：2001-01-01 00:00:00
abstract：:The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2002.0887
更新日期：2002-01-01 00:00:00
abstract：:Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1994.1029
更新日期：1994-11-01 00:00:00
abstract：:The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1996.0039
更新日期：1996-05-01 00:00:00
abstract：:In plastic and reconstructive field, it would be much beneficial to fabricate an engineered adipose tissue substitute allowing reliable and complete fat tissue regeneration. Tissue engineering chamber (TEC) holds the promise to optimize an adipogenic configuration that is efficacious as well as reproducible. A frequen...
journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1002/cbin.10731
更新日期：2017-04-01 00:00:00
abstract：:Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1994.1123
更新日期：1994-09-01 00:00:00
abstract：:Corticotropin-releasing hormone, which is the predominant regulator of neuroendocrine responses to stress, attenuates inflammation through stimulation of glucocorticoid release. Enhanced corticotropin-releasing hormone expression has been detected in inflammatory cells of the vascular endothelium, where it acts as a l...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11188
更新日期：2019-05-29 00:00:00
abstract：:Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11015
更新日期：2018-09-01 00:00:00
abstract：:The influence of maternal nicotine exposure during pregnancy and lactation on the Type II cells of lung tissue of one day old neonatal rat pups was investigated. The results clearly show that maternal nicotine exposure resulted in an increase in the type II cell count in the lungs of the offspring. In addition the lam...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1995.1075
更新日期：1995-04-01 00:00:00
abstract：:The introduction of four key transcriptional factors (CRX, RAX, NEURO-D, OTX2) allows the direct differentiation of fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination of mono-cistronic viruses. Although the combination of mono-cistronic viruses was useful, a relatively high ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10942
更新日期：2018-05-01 00:00:00
abstract：:The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1997.0207
更新日期：1998-01-01 00:00:00
abstract：:The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10006
更新日期：2013-01-01 00:00:00
abstract：:The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2001.0813
更新日期：2001-01-01 00:00:00
abstract：:The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compa...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1994.1068
更新日期：1994-04-01 00:00:00
abstract：:Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11370
更新日期：2020-08-01 00:00:00
abstract：:This review outlines the basic properties of T-tubules in skeletal muscle cells, and the factors that govern reversible vacuolation in T-tubules under experimental conditions. Comparable membranous transformations, involving the plasma membrane or occurring intracellularly, in non-muscle cells are then considered. Fin...
journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1006/cbir.2002.0945
更新日期：2002-01-01 00:00:00
abstract：:Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.07.009
更新日期：2008-10-01 00:00:00
abstract：:Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prot...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.03.001
更新日期：2007-09-01 00:00:00
abstract：:In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT/enhancer-binding protein (C/EBP) family members and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) w...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10415
更新日期：2015-04-01 00:00:00
abstract：:This report deals with observations on the directional locomotion of amoeba before and after fixation and scanning electron microscopy. The study was aimed at visualization of the stepwise events of directional movements. After the analysis of the data it is proposed that the amoeba undergoes a sequence of movement ev...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.01.009
更新日期：2007-08-01 00:00:00
abstract：:Whether fructose (FRU), as the sole energy source, confers a metabolic advantage on cancer cells against noxious stimuli is unknown. The aim of this study was to evaluate the effects of low (11 mM), moderate (25 mM), and high (55 mM) FRU concentrations alone or in combination with rotenone (ROT) or doxorubicin (DOX) i...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10653
更新日期：2016-11-01 00:00:00
abstract：:Adaptation of cardiomyocytes to chronic hypoxia in cyanotic patients remains unclear. Mitochondrial biogenesis is enhanced in myocardium from cyanotic patients, which is possibly an adaptive response. Erythropoietin (EPO) in blood and its receptor (EPOR) on cardiomyocytes are upregulated by chronic hypoxia, suggesting...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10205
更新日期：2014-03-01 00:00:00
abstract：:Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11059
更新日期：2019-02-01 00:00:00
abstract：:Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11509
更新日期：2020-11-28 00:00:00
abstract：:MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10723
更新日期：2017-03-01 00:00:00
abstract：:Fibrillin-1 is the major component of extracellular matrix microfibrils. Microfibrils dysfunction is responsible for the onset of various connective tissue diseases, including Marfan syndrome. Although ADAMTSL (a disintegrin and metalloproteinase with thrombospondin motifs-like) 6β is one of the fibrillin-1 binding pr...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11337
更新日期：2020-07-01 00:00:00
