Abstract：
:Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prothrombin and DNA synthesis and cytoskeletal changes were studied. Prothrombin inhibited DNA synthesis in hepatocytes on fibronectin, but not collagen matrix. Hepatoma cell lines were not inhibited. We found that hepatoma cell matrix conferred resistance to hepatocytes. Prothrombin decreased fibronectin but not collagen amounts, but only in the presence of hepatocytes and not hepatoma cells, indicating that it has a differential action on matrix proteins. It also caused changes in cell shape and actin depolymerization. In vivo, there was a decrease in plasma prothrombin activity after a partial hepatectomy (PH), concomitant with the peak of DNA synthesis in the hepatocytes at 24h after PH. Injection of warfarin at the time of PH, further inhibited PT activity and enhanced this 24h peak of DNA synthesis. Furthermore, repeated injection of prothrombin lowered the peak DNA synthesis after PH. The data support the hypothesis that prothrombin can act as a hepatocyte growth inhibitor, likely at the level of fibronectin loss and result in cytoskeletal changes. Hepatomas resist this action, possibly due to their different matrix proteins. This represents a novel mechanism for growth regulation and provides a possible biological significance for the tumor marker DCP.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Carr BI,Kar S,Wang M,Wang Zdoi
10.1016/j.cellbi.2007.03.001subject
Has Abstractpub_date
2007-09-01 00:00:00pages
929-38issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(07)00071-6journal_volume
31pub_type
杂志文章abstract：:Acylase 1 was investigated at the cellular level in the rat small intestine along the enterocyte-differentiation axis. As confirmed by microscopic analysis, villus tip cells and crypt cells of rat jejunal mucosa were successfully separated using the Weiser method. The proliferating undifferentiated crypt cells showed ...
journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
pub_type： 杂志文章,评审
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1995.1037
更新日期：1995-12-01 00:00:00
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
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