当前位置： SCI文献检索 > CELL BIOLOGY INTERNATIONAL期刊下所有文献 > Insulin facilitates osteoblast differentiation.

Insulin facilitates osteoblast differentiation.

Abstract：

:Tissue engineering of bone has been increasingly used in the bone defect repair. To generate osteoblasts is a major approach, and here we have examined ways of improving the efficiency of producing osteoblasts. Adipose stem cells (ADSC) were prepared from rat mesentery tissue, and transfected with Cbfa1 gene vector or/and IGF-1R gene vector. The cells were stimulated with insulin. Osteocalcin expression by the ADSCs was assessed by quantitative RT-PCR (qRT-PCR), Western blotting and enzyme-linked immunobosorbent assay. Both genes Cbfa1 and IGF-1R were transfected in ADSCs, as shown by qRT-PCR and Western blotting. Stimulation by insulin in the culture increased osteocalcin expression in ADSCs transfected by both Cbfa1 and IGF-1R, but not in those transfected with only one of these two genes. Osteocalcin in the culture supernatant was also increased by stimulation with insulin. Thus IGF-1R gene transfer together with insulin stimulation can markedly increase the efficiency of generation of osteoblasts.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Wang L,Wang H,Xu X,Wang D,Liu H

doi

10.1002/cbin.10147

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

1157-61

issue

11

eissn

1065-6995

issn

1095-8355

journal_volume

37

pub_type

杂志文章

相关文献

CELL BIOLOGY INTERNATIONAL文献大全
  • Magnesium regulates neural stem cell proliferation in the mouse hippocampus by altering mitochondrial function.

    abstract：:In the adult brain, neural stem cells from the subgranular zone (SGZ) of the hippocampus and the subventricular zone (SVZ) of the cortex progress through the following five developmental stages: radial glia-like cells, neural progenitor cells, neuroblasts, immature neurons, and mature neurons. These developmental stag...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10569

    authors： Jia S,Mou C,Ma Y,Han R,Li X

    更新日期：2016-04-01 00:00:00

  • Traditional Chinese medicine Bak Foong Pills alters uterine quiescence - possible role in alleviation of dysmenorrhoeal symptoms.

    abstract：:Since contractility of the uterus appears to be the major source of pain during dysmenorrhoea, alleviation of the contractions is believed to be a possible treatment strategy. Bak Foong Pills, a traditional Chinese formulation for use in gynaecological disorders, has long been thought as effective in the treatment of ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2009.03.003

    authors： Rowlands DK,Cui YG,Wong HY,Gou YL,Chan HC

    更新日期：2009-12-01 00:00:00

  • Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.

    abstract：:The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1997.0207

    authors： Yamamoto M,Aoyagi M,Akazawa K,Tajima S,Yamamoto K

    更新日期：1998-01-01 00:00:00

  • Prevention of intracellular oxidation in yeast: the role of vitamin E analogue, Trolox (6-hydroxy-2,5,7,8-tetramethylkroman-2-carboxyl acid).

    abstract：:Reactive oxygen species (ROS) are not only generated in conditions of cellular stress but are also constitutively produced in most cell types by specific metabolic processes. This research focused on a potential antioxidant Trolox (model compound for alpha-tocopherol), with the aim to establish exact mechanisms of Tro...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2004.11.010

    authors： Raspor P,Plesnicar S,Gazdag Z,Pesti M,Miklavcic M,Lah B,Logar-Marinsek R,Poljsak B

    更新日期：2005-01-01 00:00:00

  • Hypoxia enhances gene expression of inducible nitric oxide synthase and matrix metalloproteinase-9 in ras/myc-transformed serum-free mouse embryo cells under simulated inflammatory and infectious conditions.

    abstract：:Ras/myc-transformed serum-free mouse embryo (ras/myc SFME) cells were treated with interferon-gamma (IFN-gamma; 100 U/ml) and/or lipopolysaccharide (LPS; 0.5 microg/ml) for 24 h to simulate inflammatory and infectious conditions and investigate their effects on the expression of inducible nitric oxide synthase (iNOS) ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.04.008

    authors： Yamaguchi H,Kidachi Y,Umetsu H,Ryoyama K

    更新日期：2008-08-01 00:00:00

  • A 160 kDa protein with carbonic anhydrase activity is complexed with rubisco on the outer surface of thylakoids.

    abstract：:This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.01.010

    authors： Lazova GN,Stemler AJ

    更新日期：2008-06-01 00:00:00

  • Inhibition of Ca2+/calmodulin-dependent protein kinase or epidermal growth factor receptor tyrosine kinase abolishes lysophosphatidic acid-mediated DNA-synthesis in human myometrial smooth muscle cells.

    abstract：:Human myometrial smooth muscle cells (SMCs) were used to evaluate the proliferative activity of lysophosphatidic acid (LPA). This study specifically focuses on the role of Ca(2+)/calmodulin-dependent protein (CaM) kinase and epidermal growth factor (EGF) receptor tyrosine kinase. Myometrial SMCs were cultured from bio...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/s1065-6995(02)00352-9

    authors： Nilsson UK,Svensson SP

    更新日期：2003-01-01 00:00:00

  • Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3.

    abstract：:The purpose of this study was three fold: (1) to reveal the implications of Wnt5A for cytokine and chemokine production by human ovarian cancer cell line SKOV-3 cells, (2) to determine the influence of Wnt5A on chemotactic SKOV-3 cell migration, and (3) to assess the effect of inflammatory mediators on Wnt5A expressio...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10551

    authors： Arabzadeh S,Hossein G,Zarnani AH

    更新日期：2016-02-01 00:00:00

  • Nuclear antigens, as DNA, of DSB389 MAb and some other anti-desmin monoclonal antibodies.

    abstract：:The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1996.0099

    authors： Kamei H

    更新日期：1996-11-01 00:00:00

  • Effect of silicon dioxide on expression of poly (ADP-ribose) polymerase mRNA and protein.

    abstract：:Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2009.04.010

    authors： Gao A,Song S,Wang D,Peng W,Tian L

    更新日期：2009-07-01 00:00:00

  • Spectrin-like proteins in plant nuclei.

    abstract：:We analysed the presence and localization of spectrin-like proteins in nuclei of various plant tissues, using several anti-erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross-reactive pair of bands at 220-240 kDa, typical for human ery...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0508

    authors： De Ruijter NC,Ketelaar T,Blumenthal SS,Emons AM,Schel JH

    更新日期：2000-01-01 00:00:00

  • Intracellular localization of Xenopus small heat shock protein, hsp30, in A6 kidney epithelial cells.

    abstract：:Small heat shock proteins (shsps) are molecular chaperones that are inducible by environmental stress. In this study, immunocytochemical analysis and laser scanning confocal microscopy revealed that the shsp family, hsp30, was localized primarily in the cytoplasm of Xenopus A6 kidney epithelial cells after heat shock ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2004.12.006

    authors： Gellalchew M,Heikkila JJ

    更新日期：2005-03-01 00:00:00

  • Substrate-induced polarisation of cultured epitheliocytes and fibroblasts: non-reactivity of Ras-transformed cells.

    abstract：:Non-transformed epitheliocytes and fibroblasts undergo modulations between the less polarised and more polarised phenotypes depending on the nature of the substrate. In contrast, transformed cells express polarised phenotype regardless of the substrate. ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2001.0776

    authors： Moizhess TG,Vasiliev JM

    更新日期：2001-01-01 00:00:00

  • Tyrosine phosphorylation is involved in Ca(2+)entry in human gingival fibroblasts.

    abstract：:Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellu...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/s1065-6995(03)00125-2

    authors： Ogata Y,Nakao S,Shimizu E,Matsuda-Honjyo Y,Yamazaki M,Furuyama S,Sugiya H

    更新日期：2003-01-01 00:00:00

  • Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells.

    abstract：:Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10427

    authors： Forouzanfar M,Rabiee F,Ghaedi K,Beheshti S,Tanhaei S,Shoaraye Nejati A,Jodeiri Farshbaf M,Baharvand H,Nasr-Esfahani MH

    更新日期：2015-05-01 00:00:00

  • Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

    abstract：:The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1998.0265

    authors： Ghosh S,Paweletz N

    更新日期：1998-01-01 00:00:00

  • The role of reactive oxygen metabolites in the transcriptional regulation of IFN-gamma gene expression by histamine in NK cells following IL-2 stimulation.

    abstract：:The secretion of interferon-gamma (IFN-gamma) by natural killer (NK) cells following in vitro stimulation with interleukin-2 (IL-2) is inhibited by co-incubation with autologous monocytes at a transcriptional level by more than sixty-fold. In this study, we investigate the nature of the inhibitory signal and particula...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1996.0077

    authors： Houze TA,Larsson PA,Hellstrand K,Gustavsson B

    更新日期：1996-09-01 00:00:00

  • RIPK4 suppresses the TGF-β1 signaling pathway in HaCaT cells.

    abstract：:Receptor-interacting serine/threonine kinase 4 (RIPK4) and transforming growth factor-β 1 (TGF-β1) play critical roles in the development and maintenance of the epidermis. A negative correlation between the expression patterns of RIPK4 and TGF-β signaling during epidermal homeostasis-related events and suppression of ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11282

    authors： Dinçer T,Boz Er AB,Er İ,Toraman B,Yildiz G,Kalay E

    更新日期：2020-03-01 00:00:00

  • Suppression of ATP-induced Cl(-)secretion by enhanced expression of epithelial Na(+)channels in mouse endometrial epithelium.

    abstract：:We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2001.0754

    authors： Wang XF,Tsang LL,So SC,Chan HC

    更新日期：2001-01-01 00:00:00

  • K562 cells display different vulnerability to H₂O₂ induced oxidative stress in differing cell cycle phases.

    abstract：:Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10360

    authors： Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

    更新日期：2015-02-01 00:00:00

  • Long Non-coding RNA NEAT1 Drives the Development of Polycystic Ovary Syndrome via Sponging Multiple MicroRNAs.

    abstract：:A line of evidences have validated that multiple microRNAs (including miR-16, miR-483 and miR-324-3p) were abnormally expressed in granulosa cells, which may be closely related with the pathogenesis of polycystic ovary syndrome (PCOS). Long-chain non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11349

    authors： Sang X,Zhang Y

    更新日期：2020-03-28 00:00:00

  • Effect of bone morphogenetic protein-6 on haemopoietic stem cells and cytokine production in normal human bone marrow stroma.

    abstract：:Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the development...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0662

    authors： Ahmed N,Sammons J,Carson RJ,Khokher MA,Hassan HT

    更新日期：2001-01-01 00:00:00

  • Different disturbances--one pathway of protein unfolding. Actin folding-unfolding and misfolding.

    abstract：:This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellbi.2007.01.025

    authors： Povarova OI,Kuznetsova IM,Turoverov KK

    更新日期：2007-04-01 00:00:00

  • CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.

    abstract：:Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis and high mortality. The role of CCN5 has attracted a great focus on the regulation of cancer progression. However, the biological function and mechanism of CCN5 in OSCC are still not well elucidated. The current study was designed to determine ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11296

    authors： Sun S,Cui Z,Yan T,Wu J,Liu Z

    更新日期：2020-04-01 00:00:00

  • Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model.

    abstract：:Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11059

    authors： Chen G,Tao Q

    更新日期：2019-02-01 00:00:00

  • Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells.

    abstract：:Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10310

    authors： Pinheiro MD,Bols NC

    更新日期：2014-10-01 00:00:00

  • Molecular identification of PpHDAC1, the first histone deacetylase fron the slime mold Physarum polycephalum.

    abstract：:The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/s1065-6995(02)90933-9

    authors： Brandtner EM,Lechner T,Loidl P,Lusser A

    更新日期：2002-01-01 00:00:00

  • Differential sensitivity of human hepatoma cell line and primary rat hepatocyte culture to benzo(a)pyrene-induced unscheduled DNA synthesis and adduct formation.

    abstract：:We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1993.1083

    authors： Liu TY,Chao TW,Chiang SH,Chi CW

    更新日期：1993-04-01 00:00:00

  • Developmental regulation of granule size and numbers in larval salivary glands of drosophila by steroid hormone ecdysone.

    abstract：:The size and number of secretory granules in late larval salivary glands of Drosophila melanogaster have been related to interecdysial and early metamorphic development represented by well-known puffs in polytene chromosomes. Interecdysial period (puff stage 1 (PS1)) is characterized by presence of numerous small gran...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1999.0433

    authors： Farkas R,Suáková G

    更新日期：1999-01-01 00:00:00

  • Pioglitazone suppresses the lipopolysaccharide-induced production of inflammatory factors in mouse macrophages by inactivating NF-kappaB.

    abstract：:TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20090005

    authors： Ao C,Huo Y,Qi L,Xiong Z,Xue L,Qi Y

    更新日期：2010-07-01 00:00:00