RIPK4 suppresses the TGF-β1 signaling pathway in HaCaT cells.

Abstract:

:Receptor-interacting serine/threonine kinase 4 (RIPK4) and transforming growth factor-β 1 (TGF-β1) play critical roles in the development and maintenance of the epidermis. A negative correlation between the expression patterns of RIPK4 and TGF-β signaling during epidermal homeostasis-related events and suppression of RIPK4 expression by TGF-β1 in keratinocyte cell lines suggest the presence of a negative regulatory loop between the two factors. So far, RIPK4 has been shown to regulate nuclear factor-κB (NF-κB), protein kinase C (PKC), wingless-type MMTV integration site family (Wnt), and (mitogen-activated protein kinase) MAPK signaling pathways. In this study, we examined the effect of RIPK4 on the canonical Smad-mediated TGF-β1 signaling pathway by using the immortalized human keratinocyte HaCaT cell line. According to our results, RIPK4 inhibits intracellular Smad-mediated TGF-β1 signaling events through suppression of TGF-β1-induced Smad2/3 phosphorylation, which is reflected in the upcoming intracellular events including Smad2/3-Smad4 interaction, nuclear localization, and TGF-β1-induced gene expression. Moreover, the kinase activity of RIPK4 is required for this process. The in vitro wound-scratch assay demonstrated that RIPK4 suppressed TGF-β1-mediated wound healing through blocking TGF-β1-induced cell migration. In conclusion, our results showed the antagonistic effect of RIPK4 on TGF-β1 signaling in keratinocytes for the first time and have the potential to contribute to the understanding and treatment of skin diseases associated with aberrant TGF-β1 signaling.

journal_name

Cell Biol Int

authors

Dinçer T,Boz Er AB,Er İ,Toraman B,Yildiz G,Kalay E

doi

10.1002/cbin.11282

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

848-860

issue

3

eissn

1065-6995

issn

1095-8355

journal_volume

44

pub_type

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