Abstract:
:Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative genes were compared using the Atlas human cDNA expression array in human fibroblast cells with and without 3-dimensional (3-D) clinostat. Five upregulated and 8 downregulated genes were detected. Among these genes, upregulation of XRCC1, and downregulation of ERB-B2 and p21(Cip1/Waf1) were confirmed by RT-PCR. These results suggested that the gene expression levels of XRCC1, ERB-B2 and p21(Cip1/Waf1) were modulated by vector-averaged microgravity induced by 3-D clinostat in human fibroblast cells. Our findings may be a basis for the biological study of 3-D culture systems.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Arase Y,Nomura J,Sugaya S,Sugita K,Kita K,Suzuki Ndoi
10.1006/cbir.2001.0833keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
225-33issue
3eissn
1065-6995issn
1095-8355pii
S1065699501908339journal_volume
26pub_type
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