Abstract:
:The present study was undertaken to test the reproducible formation of the extended chromatin fibres (ECF), beads and superbeads, and detect molecular order and crystallinity by optical anisotropies in those structures. Chicken erythrocyte smears and mouse liver cell imprints were treated with 2.0-3.0 m NaCl solution in 1% Triton-X100 vertically prior to staining with 0.025% toluidine blue at pH 4. Detection of birefringence and colours of abnormal dispersion of birefringence (ADB) following toluidine blue binding to DNA revealed that the DNA molecular order and crystallinity in decondensed and condensed chromatin are preserved after ECF formation. The tests for Con-A binding to mannose/glucose residues of glycoproteins was confirmed within nuclei, and in the ECF, beads and superbeads. ECF formation was not regular. Clumped cells did not show ECF, although chromatin mobility was observed within the nuclei. Electron microscopy demonstrated that after treatment of nuclei with 0.77 m NaCl ECF always spread from the nuclei, in clumped nuclei the fibres aggregated instead of spreading.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
de Campos Vidal Bdoi
10.1006/cbir.2000.0555keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
723-8issue
10eissn
1065-6995issn
1095-8355pii
S1065-6995(00)90555-9journal_volume
24pub_type
杂志文章abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10360
更新日期:2015-02-01 00:00:00
abstract::It has been shown in many investigations that the abnormally increasing production and deposition of collagen is one of the important mechanisms of pathological scars and other fibrotic diseases [Wang Z, Inokuchi T, Nemoto TK, Uehara M, Baba TT. Antisense oligonucleotide against collagen-specific molecular chaperone 4...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.10.009
更新日期:2008-05-01 00:00:00
abstract::Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.12.004
更新日期:2004-01-01 00:00:00
abstract::ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090067
更新日期:2010-05-07 00:00:00
abstract::A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10016
更新日期:2013-02-01 00:00:00
abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11509
更新日期:2020-11-28 00:00:00
abstract::Endothelial progenitor cells (EPCs) are precursor cells of vascular endothelial cells, which are widely involved in the pathological process of cardiovascular diseases. EPCs apoptosis could accelerate the process of cardiovascular diseases. 14-3-3-η protein has been proved to be a potent antiapoptosis molecule. Howeve...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11529
更新日期:2020-12-15 00:00:00
abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
更新日期:1994-09-01 00:00:00
abstract::The function of the gene gilgamesh (89B9-12) encoding a casein kinase in Drosophila spermatogenesis was studied. The chimeric Gilgamesh-GFP protein in spermatocytes is cortically located. In the polar and apolar spermatocytes, it concentrates at the terminal ends of the fusome, the organelle that passes through the sy...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.02.009
更新日期:2009-05-01 00:00:00
abstract::The isolation and culture of articular chondrocytes is a prerequisite of their use in tissue engineering, but prolonged culture and passaging is associated with de-differentiation. In this paper we studied the influence of nanometric and micrometric grooves (85 nm to 8 microm in depth and 2 microm to 20 microm in widt...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.12.008
更新日期:2005-06-01 00:00:00
abstract::Here we report a homologue of the apolipoprotein D gene (AmphiApoD) in amphioxus, Branchiostoma belcheri tsingtauense, the first such finding in a basal chordate cephalochordate. The main features of the protein predicted from AmphiApoD are characteristic of the apolipoprotein D. Phylogenetic analysis places AmphiApoD...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.011
更新日期:2007-01-01 00:00:00
abstract::In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.006
更新日期:2006-11-01 00:00:00
abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10293
更新日期:2014-10-01 00:00:00
abstract::Although clinical data suggest remarkable promise for targeting programmed cell death protein-1 (PD-1) and ligand (PD-L1) signaling in non-small-cell lung cancer (NSCLC), it is still largely undetermined which subtype of patients will be responsive to checkpoint blockade. In the present study, we explored whether PD-L...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11524
更新日期:2020-12-15 00:00:00
abstract::Ricin is internalized after binding at the cell surface via lectin activity of the B-chain recognizing terminal galactose residues. Ricin-A chain is then translocated to the cytosol from various endocytic structures. Cell death is the result of catalytic inactivation of protein synthesis. Using (125)I-ricin overlays, ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0120
更新日期:1997-03-01 00:00:00
abstract::Human umbilical CD34(+) immature haematopoietic cells were rapidly and efficiently obtained from light density MNC (mononuclear cells) by MACS (magnetic cell sorting). An ex vivo expanded population of CD34(+) was cultured in serum-free medium supplemented with cytokines FL (flt3 ligand), SCF (stem cell factor) and TP...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090493
更新日期:2011-01-01 00:00:00
abstract::To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::With rapid development of space engineering, research on life sciences in space is being conducted extensively, especially cellular and molecular studies on space medicine. Stem cells, undifferentiated cells that can differentiate into specialized cells, are considered a key resource for regenerative medicine. Researc...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10452
更新日期:2015-06-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
更新日期:2016-04-01 00:00:00
abstract::We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no e...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.001
更新日期:2008-08-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) exists low efficiency to trans-differentiate into other germinal layer cell types. One key issue is to discover the effect of important factor on MSCs differentiation abiltiy. In this study, we investigated the role and mechanism of epiregulin (EREG) on the osteogenic differentiation and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11305
更新日期:2020-04-01 00:00:00
abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090453
更新日期:2010-10-01 00:00:00
abstract::Tissue engineering of bone has been increasingly used in the bone defect repair. To generate osteoblasts is a major approach, and here we have examined ways of improving the efficiency of producing osteoblasts. Adipose stem cells (ADSC) were prepared from rat mesentery tissue, and transfected with Cbfa1 gene vector or...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10147
更新日期:2013-11-01 00:00:00
abstract::Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11405
更新日期:2020-10-01 00:00:00
abstract::The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0583
更新日期:2001-01-01 00:00:00
abstract::Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2MG) genes was examined in the livers of rats during the acute-phase reaction. Our results demonstrate that the elevation of the Hp and...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1037
更新日期:1995-12-01 00:00:00
abstract::Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.015
更新日期:2009-09-01 00:00:00
abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11317
更新日期:2020-05-01 00:00:00
abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11059
更新日期:2019-02-01 00:00:00
abstract::GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.01.006
更新日期:2004-01-01 00:00:00