Abstract:
:We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no effect in their viability. We found that mature DCs are able to incorporate magnetic nanoparticles in a range of size from 10 nm to ca. 200 nm, after 24 h of incubation. We describe a method to separate cells loaded with NPs, and analyze the resulting material by electron microscopy and magnetic measurements. It is found that NPs are internalized in lysosomes, providing a large magnetic signal. Our results suggest that loading DCs with properly functionalized magnetic NPs could be a promising strategy for improved vectorization in cancer diagnosis and treatment.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Goya GF,Marcos-Campos I,Fernández-Pacheco R,Sáez B,Godino J,Asín L,Lambea J,Tabuenca P,Mayordomo JI,Larrad L,Ibarra MR,Tres Adoi
10.1016/j.cellbi.2008.04.001subject
Has Abstractpub_date
2008-08-01 00:00:00pages
1001-5issue
8eissn
1065-6995issn
1095-8355pii
S1065-6995(08)00380-6journal_volume
32pub_type
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