Abstract:
:We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following benzo(a)pyrene treatment, both HepG2 and primary rat hepatocyte culture showed unscheduled DNA synthesis with different sensitivity. In 32P-postlabelling analysis, the chromatogram revealed quantitative and qualitative differences between HepG2 and primary rat hepatocyte cultures when treated with 10 microM benzo(a)pyrene for 18 hr. The results have demonstrated that the HepG2 cell line may be used in addition to primary rat hepatocytes in risk assessment for detection of environmental carcinogens.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Liu TY,Chao TW,Chiang SH,Chi CWdoi
10.1006/cbir.1993.1083subject
Has Abstractpub_date
1993-04-01 00:00:00pages
441-7issue
4eissn
1065-6995issn
1095-8355pii
S1065-6995(83)71083-8journal_volume
17pub_type
杂志文章abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11059
更新日期:2019-02-01 00:00:00
abstract::Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10258
更新日期:2014-06-01 00:00:00
abstract::ROS (reactive oxygen species) take an important signalling role in angiogenesis. Although there are several ways to produce ROS in cells, multicomponent non-phagocytic NADPH oxidase is an important source of ROS that contribute to angiogenesis. In the present work, we examined the effects of H2O2 on angiogenesis inclu...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090092
更新日期:2010-10-01 00:00:00
abstract::A line of evidences have validated that multiple microRNAs (including miR-16, miR-483 and miR-324-3p) were abnormally expressed in granulosa cells, which may be closely related with the pathogenesis of polycystic ovary syndrome (PCOS). Long-chain non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11349
更新日期:2020-03-28 00:00:00
abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1077
更新日期:1993-04-01 00:00:00
abstract::Rb/E2F regulates many genes that encode proteins required for the cell cycle. Using affymetrix microarrays we previously identified genes regulated by the Rb proteins p130 and p107, many of which are involved in the cell cycle. Several genes with unknown functions were also repressed by p130 and p107, of which some ha...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.013
更新日期:2007-12-01 00:00:00
abstract::Neural stem cells (NSCs) are currently considered powerful candidates for cell therapy in neurodegenerative disorders such as Parkinson's disease. However, it is not known when and how NSCs begin to differentiate functionally. Recent reports suggest that classical neurotransmitters such as acetylcholine (Ach) are invo...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.10.005
更新日期:2004-01-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.011
更新日期:2008-01-25 00:00:00
abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
更新日期:1994-09-01 00:00:00
abstract::Aerenchyma is a plant tissue characterized by prominent intercellular spaces facilitating gas diffusion between roots and the aerial environment. The classical formation of intercellular spaces is thought to be the result of schizogeny and lysogeny during development of wetland species and in some dry-land species in ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10814
更新日期:2019-09-01 00:00:00
abstract::Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this r...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10581
更新日期:2016-05-01 00:00:00
abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1042/CBI20100514
更新日期:2010-11-01 00:00:00
abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11509
更新日期:2020-11-28 00:00:00
abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11437
更新日期:2020-11-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facin...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100927
更新日期:2011-10-01 00:00:00
abstract::The isolation and culture of articular chondrocytes is a prerequisite of their use in tissue engineering, but prolonged culture and passaging is associated with de-differentiation. In this paper we studied the influence of nanometric and micrometric grooves (85 nm to 8 microm in depth and 2 microm to 20 microm in widt...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.12.008
更新日期:2005-06-01 00:00:00
abstract::Metallothionein (MT), a cysteine-rich, metal-binding protein, is involved in homeostatic regulation of essential metals and protection of cells against oxidative injury. It has been shown that oxidative stress is associated with pathogenesis of osteoporosis and is capable of inhibiting osteoblastic differentiation of ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.004
更新日期:2004-01-01 00:00:00
abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0813
更新日期:2001-01-01 00:00:00
abstract::Cutaneous squamous cell carcinoma (SCC) is a malignant tumour of keratinising epidermal cells. This type of skin cancer is the second leading cause of death after melanoma, and it is the second most common type of non-melanoma skin cancer after basal cell carcinoma. The cellular and molecular events involved in the pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.006
更新日期:2007-11-01 00:00:00
abstract::As experimental evidence suggests that leptin may have direct effects on peripheral tissues, we investigated some of the transductional molecules induced by leptin in C2C12 cells. In immunoprecipitation experiments using anti-p85 antibodies (a regulatory subunit of phosphatidylinositol-3-kinase; PI3K), we observed a s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.03.008
更新日期:2005-07-01 00:00:00
abstract::Several synthetic decapeptides containing an HAV tripeptide motif were tested for their ability to modulate the enzymatic activity of rat extracellular SOD, an enzyme which also contains an HAV motif. Out of nine decapeptides that were tested, only a FGF-receptor derived peptide was active as a negative modulator of e...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1032
更新日期:1995-11-01 00:00:00
abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10568
更新日期:2016-03-01 00:00:00
abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.006
更新日期:2007-03-01 00:00:00
abstract::Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0760
更新日期:2001-01-01 00:00:00
abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.012
更新日期:2005-02-01 00:00:00
abstract::ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090067
更新日期:2010-05-07 00:00:00
abstract::To better treat patients with non-small cell lung cancer (NSCLC), the investigations on novel molecules affecting NSCLC progression are of vital importance. Long noncoding RNAs (lncRNAs) are identified as pivotal regulators that can affect the cellular activities of carcinomas. Long intergenic non-protein coding RNA 6...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11361
更新日期:2020-08-01 00:00:00
abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090453
更新日期:2010-10-01 00:00:00
abstract::Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.009
更新日期:2008-10-01 00:00:00