The neurosteroid dehydroepiandrosterone could improve somatic cell reprogramming.

Abstract:

:Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facing the successful reprogramming and clinical translation of iPSC technology is the high rate of apoptosis after the reprogramming process. Reprogramming is a stressful process, and the p53 apoptotic pathway plays a negative role in cell growth and self-renewal. Apoptosis via the p53 pathway serves as a major barrier in nuclear somatic cell reprogramming during iPSC generation. DHEA (dehydroepiandrosterone) is an abundant steroid that is produced at high levels in the adrenal cells, and withdrawal of DHEA increases the levels of p53 in the epithelial and stromal cells, resulting in increased levels of apoptotic cells; meanwhile, DHEA decreases cellular apoptosis. DHEA could improve the efficacy of reprogramming yield due to a decrease in apoptosis via the p53 pathway and an increase in cell viability.

journal_name

Cell Biol Int

authors

Shoae-Hassani A,Sharif S,Verdi J

doi

10.1042/CBI20100927

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1037-41

issue

10

eissn

1065-6995

issn

1095-8355

pii

CBI20100927

journal_volume

35

pub_type

杂志文章
  • Mechanical aspects of cell shape regulation and signaling.

    abstract::Physical forces play a critical role in cell integrity and development, but little is known how cells convert mechanical signals into biochemical responses. This mini-review examines potential molecular mediators like integrins, focal adhesion proteins, and the cytoskeleton in the context of a complex cell structure. ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1006/cbir.2002.0857

    authors: Goldmann WH

    更新日期:2002-01-01 00:00:00

  • Synaptic modulation of neuronal coupling.

    abstract::Electrotonic coupling among neurons in the vertebrate, and more specifically the mammalian, brain has now been demonstrated to exist in all major brain subdivisions and in the spinal cord. For many of these brain areas, recent studies have investigated the possibilities of modulation of that coupling by synaptically r...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1006/cbir.1998.0386

    authors: Hatton GI

    更新日期:1998-11-01 00:00:00

  • K562 cells display different vulnerability to H₂O₂ induced oxidative stress in differing cell cycle phases.

    abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10360

    authors: Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

    更新日期:2015-02-01 00:00:00

  • Traditional Chinese medicine Bak Foong Pills alters uterine quiescence - possible role in alleviation of dysmenorrhoeal symptoms.

    abstract::Since contractility of the uterus appears to be the major source of pain during dysmenorrhoea, alleviation of the contractions is believed to be a possible treatment strategy. Bak Foong Pills, a traditional Chinese formulation for use in gynaecological disorders, has long been thought as effective in the treatment of ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2009.03.003

    authors: Rowlands DK,Cui YG,Wong HY,Gou YL,Chan HC

    更新日期:2009-12-01 00:00:00

  • Effects of spaceflight conditions on fertilization and embryogenesis in the sea urchin Lytechinus pictus.

    abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0371

    authors: Schatten H,Chakrabarti A,Taylor M,Sommer L,Levine H,Anderson K,Runco M,Kemp R

    更新日期:1999-01-01 00:00:00

  • Stem cells conditioned medium: a new approach to skin wound healing management.

    abstract::Stem cell biology has gained remarkable interest in recent years, driven by the hope of finding cures for numerous diseases including skin wound healing through transplantation medicine. Initially upon transplantation, these cells home to and differentiate within the injured tissue into specialised cells. Contrariwise...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10138

    authors: Jayaraman P,Nathan P,Vasanthan P,Musa S,Govindasamy V

    更新日期:2013-10-01 00:00:00

  • Intracellular localization of Xenopus small heat shock protein, hsp30, in A6 kidney epithelial cells.

    abstract::Small heat shock proteins (shsps) are molecular chaperones that are inducible by environmental stress. In this study, immunocytochemical analysis and laser scanning confocal microscopy revealed that the shsp family, hsp30, was localized primarily in the cytoplasm of Xenopus A6 kidney epithelial cells after heat shock ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.12.006

    authors: Gellalchew M,Heikkila JJ

    更新日期:2005-03-01 00:00:00

  • MiR-21 inhibitor suppressed the progression of retinoblastoma via the modulation of PTEN/PI3K/AKT pathway.

    abstract::MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little was revealed about the function of miR-21 in retinoblastoma (RB). In this study, we examined the expression of miR-21 in RB tissues and explored the relationship between miR-21 and phosphatase and tensi...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10678

    authors: Gui F,Hong Z,You Z,Wu H,Zhang Y

    更新日期:2016-12-01 00:00:00

  • Expression of glutamyl aminopeptidase by osteogenic induction in rat bone marrow stromal cells.

    abstract::Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.02.006

    authors: Wu Y,Xiao J,Wu L,Tian W,Liu L

    更新日期:2008-07-01 00:00:00

  • vasa-related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala).

    abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.09.012

    authors: Shukalyuk AI,Golovnina KA,Baiborodin SI,Gunbin KV,Blinov AG,Isaeva VV

    更新日期:2007-02-01 00:00:00

  • Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.

    abstract::Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs cons...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.04.025

    authors: Zhou JM,Xing FY,Shi JJ,Fang ZF,Chen XJ,Chen F

    更新日期:2008-09-01 00:00:00

  • Inability of 50 Hz magnetic fields to regulate PKC- and Ca(2+)-dependent gene expression in Jurkat cells.

    abstract::We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56(lck), increase in inositol-3-phosphate levels and increase in intracellular calcium concentration...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0837

    authors: Still M,Lindström E,Ekstrand AJ,Mild KH,Mattsson MO,Lundgren E

    更新日期:2002-01-01 00:00:00

  • All-trans retinoic acid displays multiple effects on the growth, lipogenesis and adipokine gene expression of AML-I preadipocyte cell line.

    abstract::Adipose tissue is a potential site of retinoic acid (RA) action, but its physiological significance remains to be clarified. We have examined the effect of all-trans retinoic acid (ATRA) on growth and differentiation of preadipocytes, and on adipokine gene expression in mature adipocytes using human preadipocyte cell ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10005

    authors: Morikawa K,Hanada H,Hirota K,Nonaka M,Ikeda C

    更新日期:2013-01-01 00:00:00

  • Low-dose aspirin promotes endothelial progenitor cell migration and adhesion and prevents senescence.

    abstract::Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.03.004

    authors: Hu Z,Zhang F,Yang Z,Zhang J,Zhang D,Yang N,Zhang Y,Cao K

    更新日期:2008-07-01 00:00:00

  • Insulin-producing cells from human pancreatic islet-derived progenitor cells following transplantation in mice.

    abstract::Stem/progenitor cells hold promise for alleviating/curing type 1 diabetes due to the capacity to differentiate into functional insulin-producing cells. The current study aims to assess the differentiation potential of human pancreatic IPCs (islet-derived progenitor cells). IPCs were derived from four human donors and ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100152

    authors: Zhang Y,Ren Z,Zou C,Wang S,Luo B,Li F,Liu S,Zhang YA

    更新日期:2011-05-01 00:00:00

  • Vibration activates the actin/NF-κB axis and upregulates IL-6 and IL-8 expression in human periodontal ligament cells.

    abstract::We previously reported that mechanical vibration-induced proinflammatory cytokines, interleukin-6 (IL-6) and IL-8, expression in human periodontal ligament (hPDL) cells, however, the underlying mechanism remained unclear. Mechanical stimuli are able to activate cellular responses by inducing the activation of several ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11267

    authors: Phusuntornsakul P,Jitpukdeebodintra S,Pavasant P,Leethanakul C

    更新日期:2020-02-01 00:00:00

  • Microbiological contamination in stem cell cultures.

    abstract::Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.03.010

    authors: Cobo F,Cortés JL,Cabrera C,Nieto A,Concha A

    更新日期:2007-09-01 00:00:00

  • Validation of common reference genes stability in exosomal mRNA-isolated from liver and breast cancer cell lines.

    abstract::Accurate relative gene expression analysis by reverse transcription-quantitative polymerase chain reaction relies on the usage of suitable reference genes for data normalization. The RNA content of small extracellular vesicles including exosomes is growingly considered as cancer biomarkers. So, reliable relative quant...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11556

    authors: Gorji-Bahri G,Moradtabrizi N,Vakhshiteh F,Hashemi A

    更新日期:2021-01-27 00:00:00

  • Digital image analysis of chromatin fibre phenotype after "in situ' digestion with restriction endonucleases.

    abstract::Restriction Endonucleases (REs) may recognize, cleave and remove DNA from fixed chromatin producing specific chromosome banding patterns. However, the modifications produced in the chromatin fibre are not easy to evaluate and compare. The aim of the present investigation was to visualize differences resulting in the t...

    journal_title:Cell biology international

    pub_type: 更正并重新发布的文章,杂志文章

    doi:10.1006/cbir.1996.0026

    authors: Gosálvez J,López-Fernández C,Fernández JL,Goyanes VJ,Buño I

    更新日期:1996-03-01 00:00:00

  • Significance of eIF4E expression in skin squamous cell carcinoma.

    abstract::Cutaneous squamous cell carcinoma (SCC) is a malignant tumour of keratinising epidermal cells. This type of skin cancer is the second leading cause of death after melanoma, and it is the second most common type of non-melanoma skin cancer after basal cell carcinoma. The cellular and molecular events involved in the pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.06.006

    authors: Salehi Z,Mashayekhi F,Shahosseini F

    更新日期:2007-11-01 00:00:00

  • Alternatively spliced forms of the P180 ribosome receptor differ in their ability to induce the proliferation of rough endoplasmic reticulum.

    abstract::Expression of the canine 180-kDa ribosome receptor p180 in yeast induces the synthesis of RER, and increases the mRNAs of secretory pathway proteins, and protein secretion. To assess whether p180 is a master regulator of cell secretion in mammalian cells, we stably expressed red fluorescent forms of the human p180 var...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.10.002

    authors: Bai JZ,Leung E,Holloway H,Krissansen GW

    更新日期:2008-05-01 00:00:00

  • Bacterial invasion of eukaryotic cells can be mediated by actin-hydrolysing metalloproteases grimelysin and protealysin.

    abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100314

    authors: Bozhokina ES,Tsaplina OA,Efremova TN,Kever LV,Demidyuk IV,Kostrov SV,Adam T,Komissarchik YY,Khaitlina SY

    更新日期:2011-02-01 00:00:00

  • Suppression of ATP-induced Cl(-)secretion by enhanced expression of epithelial Na(+)channels in mouse endometrial epithelium.

    abstract::We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0754

    authors: Wang XF,Tsang LL,So SC,Chan HC

    更新日期:2001-01-01 00:00:00

  • Rosbin, a synthetic small molecule, induces A549 cells apoptosis through a ROS-mediated pathway.

    abstract::Rosbin (Thieno [2, 3-c] piperidine-3-carboxamide-2-[(3-methoxy-naphtha-lene-2-carbonyl)-amino]-6-(benzyl)-, hydrochloride), a synthetic piperidinothieno derivative compound obtained from chemical genetics screenings, significantly suppresses the viability of non-small cell lung cancer A549 cells with an IC50 of 2.05 ±...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10714

    authors: Zhao T,Jin W,Pan H,Li H,Zhao Y,Feng Y

    更新日期:2017-02-01 00:00:00

  • Plant tubulin intronics.

    abstract::Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5'UTR sequence of some rice beta-tubulin genes can...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.11.013

    authors: Breviario D,Gianì S,Ponzoni E,Mastromauro F,Morello L

    更新日期:2008-05-01 00:00:00

  • Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena.

    abstract::FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.06.019

    authors: Csaba G,Kovács P,Tóthfalusi L,Pállinger E

    更新日期:2006-12-01 00:00:00

  • Serpin A1 and the modulation of type I collagen turnover: Effect of the C-terminal peptide 409-418 (SA1-III) in human dermal fibroblasts.

    abstract::The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III incre...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11018

    authors: Cipriani C,Pascarella S,Errante F,Menicacci B,Magnelli L,Mocali A,Rovero P,Giovannelli L

    更新日期:2018-09-01 00:00:00

  • Ro52/SSA sensitizes cells to death receptor-induced apoptosis by down-regulating c-FLIP(L).

    abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110322

    authors: Zhang J,Fang L,Zhu X,Qiao Y,Yu M,Wang L,Chen Y,Yin W,Hua ZC

    更新日期:2012-05-01 00:00:00

  • HGF expression induced by HIF-1α promote the proliferation and tube formation of endothelial progenitor cells.

    abstract::Hypoxia-inducible factor-1α (HIF-1α) and hepatocyte growth factor (HGF) play important roles in postnatal neovascularization. However, the interaction of these two pathways is not fully understood. The present study utilized CoCl(2) treated-endothelial progenitor cells (EPCs) (EPCs exposure to CoCl(2) are under mimic ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10397

    authors: Yu F,Lin Y,Zhan T,Chen L,Guo S

    更新日期:2015-03-01 00:00:00

  • Retinoic acid induces myoblasts transdifferentiation into premeiotic Stra8-positive cells.

    abstract::Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100610

    authors: Jia W,Cheng D,Chen S,Lei L,Wang H

    更新日期:2011-04-01 00:00:00