Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.

abstract：:While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...

journal_title：Cell biology international

authors： Padros MR,Giles DM,Suarez MD

更新日期：1997-06-01 00:00:00

abstract：:The aim of the present study was to determine the effects of ARHI (aplasia Ras homologue member I; also known as DIRAS3), a member of the Ras superfamily, on HCC (hepatocellular carcinoma) cells and to define the molecular pathways involved. Stable transfection of ARHI into the HCC cell line Hep3B that lacks expressio...

journal_title：Cell biology international

authors： Pei XH,Yang Z,Liu HX,Qiao SS

更新日期：2011-10-01 00:00:00

abstract：:In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only...

journal_title：Cell biology international

authors： Cheng XR,Zhang L,Hu JJ,Sun L,Du GH

更新日期：2007-05-01 00:00:00

abstract：:Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...

journal_title：Cell biology international

authors： Dalby MJ,Riehle MO,Johnstone H,Affrossman S,Curtis AS

更新日期：2004-01-01 00:00:00

abstract：:Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...

journal_title：Cell biology international

authors： Filiz S,Dalcik H,Yardimoglu M,Gonca S,Ceylan S

更新日期：2002-01-01 00:00:00

abstract：:Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...

journal_title：Cell biology international

authors： Maftoum-Costa M,Naves KT,Oliveira AL,Tedesco AC,da Silva NS,Pacheco-Soares C

更新日期：2008-08-01 00:00:00

abstract：:Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mecha...

journal_title：Cell biology international

authors： Zhang Y,Zhang Y

更新日期：2018-07-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...

journal_title：Cell biology international

authors： Qu Y,Wang Y,Wang P,Lin N,Yan X,Li Y

更新日期：2020-08-01 00:00:00

abstract：:Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

journal_title：Cell biology international

authors： Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

更新日期：2020-02-01 00:00:00

abstract：:TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis...

journal_title：Cell biology international

authors： Ao C,Huo Y,Qi L,Xiong Z,Xue L,Qi Y

更新日期：2010-07-01 00:00:00

abstract：:The unicellular Tetrahymena has receptors for hormones of higher vertebrates, produces these hormones, and their signal pathways are similar. The first encounter with a hormone in higher dose provokes the phenomenon of hormonal imprinting, by which the reaction of the cell is quantitatively modified. This modification...

journal_title：Cell biology international

authors： Kőhidai L,Lajkó E,Pállinger E,Csaba G

更新日期：2012-10-01 00:00:00

abstract：:Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...

journal_title：Cell biology international

authors： Wan Q,Lu H,Wu LT,Liu X,Xiang JB

更新日期：2014-12-01 00:00:00

abstract：:The purpose of this study was three fold: (1) to reveal the implications of Wnt5A for cytokine and chemokine production by human ovarian cancer cell line SKOV-3 cells, (2) to determine the influence of Wnt5A on chemotactic SKOV-3 cell migration, and (3) to assess the effect of inflammatory mediators on Wnt5A expressio...

journal_title：Cell biology international

authors： Arabzadeh S,Hossein G,Zarnani AH

更新日期：2016-02-01 00:00:00

abstract：:Under the 1G condition, the increase in antipain-sensitive protease activity promptly after UV (mainly 254 nm wavelength) irradiation in cultured human cells is detected and found to be one of the intriguing events involved in suppression of cell mutability. It was found that two cell lines, RSa and its variant UVAP-1...

journal_title：Cell biology international

authors： Takahashi S,Zhang HC,Hasegawa R,Karata K,Kita K,Wano C,Yamaguchi Y,Sugaya S,Nomura J,Ichinose M,Suzuki N

更新日期：2003-01-01 00:00:00

abstract：:The effect of piperine on the fertilization of eggs with sperm was investigated in female hamsters. They were intragastrically treated with piperine at doses of 50 and 100 mg/kg BW from day 1 through day 4 of the oestrous cycle. During piperine treatment, these females were superovulated and artificially inseminated (...

journal_title：Cell biology international

authors： Piyachaturawat P,Pholpramool C

更新日期：1997-07-01 00:00:00

abstract：:The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...

journal_title：Cell biology international

authors： Cetintas VB,Tetik A,Cok G,Kucukaslan AS,Kosova B,Gunduz C,Veral A,Eroglu Z

更新日期：2013-01-01 00:00:00

abstract：:Mammalian cells in vivo frequently express primary cilia. Although some fully differentiated cell types rarely, if ever, express them, most do, indicating that they are regular cell organelles. Their expression can also be explored in vitro, where conditions--physical and chemical, intrinsic and extrinsic--permit expe...

journal_title：Cell biology international

authors： Wheatley DN,Wang AM,Strugnell GE

更新日期：1996-01-01 00:00:00

abstract：:Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-...

journal_title：Cell biology international

authors： Jia W,Cheng D,Chen S,Lei L,Wang H

更新日期：2011-04-01 00:00:00

abstract：:Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtai...

journal_title：Cell biology international

authors： Tedeschi G,Bernardini G,Mortarino M,Negri A,Ceciliani F,Ronchi S

更新日期：1998-01-01 00:00:00

abstract：:The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence inclu...

journal_title：Cell biology international

authors： Nie YW,Ding XB,Ge XG,Fan HL,Liu ZW,Guo H

更新日期：2013-09-01 00:00:00

abstract：:ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...

journal_title：Cell biology international

authors： Huang HJ,Gao QS,Qian YG,Zhang YD,Tao BF,Xiang M,Peng J,Jiang SW,Hause B

更新日期：2010-10-01 00:00:00

abstract：:We have investigated constitutive endocytosis in internodal cells of the characean green algae. The endocytic tracer FM1-43 accumulated in distinct plasma membrane domains that are probably enriched in sterol-like substances. Internalization of the dye was active but independent of an intact actin or microtubule cytos...

journal_title：Cell biology international

authors： Foissner I,Klima A

更新日期：2008-05-01 00:00:00

abstract：:Life cannot be simply defined in biochemical terms but it is associated with autodynamic behaviour. This fact implies that all aspects of cell biology should be viewed in terms of the resultant temporal features. Theoretical arguments indicate that the dynamic state can be explained only by the existence of periodicit...

journal_title：Cell biology international

authors： Gilbert D,Lloyd D

更新日期：2000-01-01 00:00:00

abstract：:Ras/myc-transformed serum-free mouse embryo (ras/myc SFME) cells were treated with interferon-gamma (IFN-gamma; 100 U/ml) and/or lipopolysaccharide (LPS; 0.5 microg/ml) for 24 h to simulate inflammatory and infectious conditions and investigate their effects on the expression of inducible nitric oxide synthase (iNOS) ...

journal_title：Cell biology international

authors： Yamaguchi H,Kidachi Y,Umetsu H,Ryoyama K

更新日期：2008-08-01 00:00:00

abstract：:Mutants of the methanol-utilizing yeast Pichia pastoris and the alkane-utilizing yeast Yarrowia lipolytica defective in the orthologue of UGT51 (encoding sterol glucosyltransferase) were isolated and compared. These mutants do not contain the specific ergosterol derivate, ergosterol glucoside. We observed that the P. ...

journal_title：Cell biology international

authors： Stasyk OV,Nazarko TY,Stasyk OG,Krasovska OS,Warnecke D,Nicaud JM,Cregg JM,Sibirny AA

更新日期：2003-01-01 00:00:00

abstract：:Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...

journal_title：Cell biology international

authors： Grzelak A,Mazur J,Bartosz G

更新日期：2001-01-01 00:00:00

abstract：:We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The meth...

journal_title：Cell biology international

authors： Forer A,Pickett-Heaps J

更新日期：2005-09-01 00:00:00

abstract：:The size and number of secretory granules in late larval salivary glands of Drosophila melanogaster have been related to interecdysial and early metamorphic development represented by well-known puffs in polytene chromosomes. Interecdysial period (puff stage 1 (PS1)) is characterized by presence of numerous small gran...

journal_title：Cell biology international

authors： Farkas R,Suáková G

更新日期：1999-01-01 00:00:00

abstract：:Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...

journal_title：Cell biology international

authors： Elinos-Báez CM,León F,Santos E

更新日期：2005-08-01 00:00:00

abstract：:The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and ma...

journal_title：Cell biology international

authors： Jin W,Li Q,Wang J,Chang G,Lin Y,Li H,Wang L,Gao W,Pang T

更新日期：2012-08-01 00:00:00