Abstract:
:Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5'UTR sequence of some rice beta-tubulin genes can sustain IME in rice transgenic plants and that degenerated oligonucleotide mixtures designed to amplify the first and the second intron present within the coding sequence of plant beta-tubulin genes can successfully detect ILPs among different bean varieties.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Breviario D,Gianì S,Ponzoni E,Mastromauro F,Morello Ldoi
10.1016/j.cellbi.2007.11.013subject
Has Abstractpub_date
2008-05-01 00:00:00pages
571-3issue
5eissn
1065-6995issn
1095-8355pii
S1065-6995(07)00263-6journal_volume
32pub_type
杂志文章abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1039
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abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme of the glycolytic pathway. Recent studies have demonstrated an additional role in apoptosis: GAPDH is targeted to the nucleus during apoptotic signalling. This nuclear transport has also been observed in serum-depleted cells, but it is reversible in fibr...
journal_title:Cell biology international
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journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...
journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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journal_title:Cell biology international
pub_type: 杂志文章
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abstract::Accurate relative gene expression analysis by reverse transcription-quantitative polymerase chain reaction relies on the usage of suitable reference genes for data normalization. The RNA content of small extracellular vesicles including exosomes is growingly considered as cancer biomarkers. So, reliable relative quant...
journal_title:Cell biology international
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abstract::A number of pathophysiological conditions are related to iron metabolism disturbances. Some of them are well known, others are newly discovered or special. Hepcidin is a newly identified iron metabolism regulating hormone, which could be a promising biomarker for many disorders. In this review, we provide background i...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10505
更新日期:2015-11-01 00:00:00
abstract::To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [75 PDL (population doubli...
journal_title:Cell biology international
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更新日期:2012-06-01 00:00:00
abstract::It has been reported that chronic inflammation of the vessel wall is a hallmark of atherosclerosis. Interleukin-6 (IL-6) is regarded as an important modulator of inflammatory events occurring during all stages of atherogenesis. Although many factors that induce IL-6 expression have been identified, the effect of homoc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.007
更新日期:2006-07-01 00:00:00
abstract::Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtai...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0296
更新日期:1998-01-01 00:00:00
abstract::Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Sev...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0517
更新日期:2000-01-01 00:00:00
abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1042/CBI20100514
更新日期:2010-11-01 00:00:00
abstract::Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11232
更新日期:2019-09-09 00:00:00
abstract::Stem cell biology has gained remarkable interest in recent years, driven by the hope of finding cures for numerous diseases including skin wound healing through transplantation medicine. Initially upon transplantation, these cells home to and differentiate within the injured tissue into specialised cells. Contrariwise...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10138
更新日期:2013-10-01 00:00:00
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journal_title:Cell biology international
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doi:10.1002/cbin.10069
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journal_title:Cell biology international
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.010
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10989
更新日期:2018-09-01 00:00:00
abstract::Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090063
更新日期:2010-02-22 00:00:00
abstract::Previous trials performed in our laboratory have demonstrated that stainless steel corrosion products induced morphological changes in mice seminiferous cells. In this study the functional nature of the blood-testis barrier was investigated, in view of the extensive use of this metallic alloy in orthopaedic surgery. T...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1109
更新日期:1995-07-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
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abstract::Human tissues are sophisticated ensembles of various distinct cell types encapsulated in the biomechanical cues of the extracellular matrix. It has been known matrix stiffness plays a pivot role in cellular events and tissue-scale biological processes. Thus, materials that can mimic mechanical environments of tissues ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
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更新日期:2019-02-01 00:00:00
abstract::In this study, we determined whether multilineage-differentiating stress-enduring (Muse) cells exist in rat bone marrow and elucidated their effects on protection against the injury of intestinal epithelial cells associated with inflammation. Rat Muse cells were separated from bone marrow mesenchymal stem cells (BMMSC...
journal_title:Cell biology international
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abstract::CLIC5 (chloride intracellular channel 5) is a CLIC (chloride intracellular channel) with various functions. Its high expression in skeletal muscle and association with actin-based cytoskeleton suggests that it may play an important role in muscle tissue. This study was conducted to examine whether CLIC5 regulates the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090334
更新日期:2010-03-08 00:00:00
abstract::The purpose of the study was to examine the effect of interleukins, IL-6, IL-8, and IL-15, on insulin-mediated redistribution of Rab4a, an early endosome marker, in mouse 3T3-L1 adipocytes. The interleukins did not affect cell viability; however, cell number was slightly but significantly higher in cultures exposed to...
journal_title:Cell biology international
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