Two-dimensional protein maps of xenopus eggs and embryos at different developmental stages.

Abstract:

:Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtained in four different ways: (1) immuno-blotting; (2) amino terminal sequence after blotting on to PVDF membranes; (3) comigration; and (4) assignment in comparison with proteins separated by 2D techniques on reference maps such as human liver, red blood cells, plasma and cerebrospinal fluid reported in the Swiss 2D-PAGE Data Base. The maps presented in this report are a step toward the study of the protein expression in Xenopus eggs and embryos and may be a powerful working tool since Xenopus embryos are popular models for the study of development.

journal_name

Cell Biol Int

authors

Tedeschi G,Bernardini G,Mortarino M,Negri A,Ceciliani F,Ronchi S

doi

10.1006/cbir.1998.0296

keywords:

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

517-25

issue

7-8

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(98)90296-7

journal_volume

22

pub_type

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