Abstract：
:Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential form of contamination in these cell products is by bacteria (including Mycoplasma), yeast and fungi. In our study, 32 stem cell lines and feeder cell lines were analysed. There were 19 contaminated cell passages (12%). The main contaminants were gram positive cocci and Mycoplasma species, followed by gram negative rods and gram positive rods. The Mycoplasma contamination rate was 4%. Stem cell banks and other research centres aim to screen all processed stem cell lines for these microorganisms, and to assure that no contaminants are introduced in the banking procedures. It is a standard part of current good practice in stem cell banks to carry out routine microbiological controls of the stem cell lines and to work in a controlled environment to reduce the probability of contamination in the final product.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Cobo F,Cortés JL,Cabrera C,Nieto A,Concha Adoi
10.1016/j.cellbi.2007.03.010subject
Has Abstractpub_date
2007-09-01 00:00:00pages
991-5issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(07)00080-7journal_volume
31pub_type
杂志文章abstract：:This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...
journal_title：Cell biology international
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Cell biology international
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2002.0877
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2000.0706
更新日期：2001-01-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10461
更新日期：2015-08-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.03.016
更新日期：2005-08-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1006/cbir.2002.0857
更新日期：2002-01-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.03.018
更新日期：2005-08-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2001.0776
更新日期：2001-01-01 00:00:00
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journal_title：Cell biology international
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1006/cbir.1996.0026
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.11.002
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journal_title：Cell biology international
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doi：10.1006/cbir.1993.1071
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pub_type： 杂志文章
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