Gelatinases and metalloproteinase inhibitor secreted by murine colonic carcinoma cells with differing metastatic potential.

Abstract:

:Gelatinase activity and inhibitory activity against collagenase were measured in serum-free medium conditioned by murine colonic carcinoma cells with different spontaneous metastatic potentials to the lung. The medium conditioned with poorly metastatic NM11 cells gave higher inhibitory activity than that conditioned with highly metastatic LuM1 cells, while the level of secreted gelatinases in the same medium was lower in NM11 medium than in LuM1 case. Northern analysis showed the higher gene expression of both tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 in NM11 cells than in LuM1 cells, suggesting that both TIMPs are responsible for the increase of inhibitory activity in NM11 conditioned medium. Examination of the balance of gelatinases and inhibitor revealed that the amount of inhibitor exceeded that of gelatinases in the medium conditioned with NM11 cells. In contrast, the medium conditioned with LuM1 cells contained excess amounts of gelatinases. The results indicated a close correlation between the balance of gelatinases and inhibitors and the metastatic behavior of murine tumor cells.

journal_name

Cell Biol Int

authors

Kishi J,Tanaka R,Koiwai O,Yamagata S,Numata Y,Hayakawa T,Shimizu S

doi

10.1006/cbir.1994.1057

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

165-70

issue

3

eissn

1065-6995

issn

1095-8355

pii

S1065699584710572

journal_volume

18

pub_type

杂志文章
  • Hepcidin and its potential clinical utility.

    abstract::A number of pathophysiological conditions are related to iron metabolism disturbances. Some of them are well known, others are newly discovered or special. Hepcidin is a newly identified iron metabolism regulating hormone, which could be a promising biomarker for many disorders. In this review, we provide background i...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10505

    authors: Miseta A,Nagy J,Nagy T,Poór VS,Fekete Z,Sipos K

    更新日期:2015-11-01 00:00:00

  • Hypoxia affects positively the proliferation of bovine satellite cells and their myogenic differentiation through up-regulation of MyoD.

    abstract::Hypoxia alters the biological functions of skeletal muscle cells to proliferate and differentiate into myotubes. However, the cellular responses of myoblasts to hypoxia differ according to the levels of oxygen and the types of cells studied. This study examined the effect of hypoxia (1% oxygen) on bovine satellite cel...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.03.017

    authors: Kook SH,Son YO,Lee KY,Lee HJ,Chung WT,Choi KC,Lee JC

    更新日期:2008-08-01 00:00:00

  • Nuclear deformation characterizes Werner syndrome cells.

    abstract::Mutations in the lamin A gene have been shown, among other defects, to give rise to Hutchinson-Gilford progeria syndrome (HGPS) and to atypical Werner syndrome (WS), both of which are progeroid disorders. Here, we have investigated well-characterized WS patient cell strains that are compound heterozygous for mutations...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.10.011

    authors: Adelfalk C,Scherthan H,Hirsch-Kauffmann M,Schweiger M

    更新日期:2005-12-01 00:00:00

  • Spectrin-like proteins in plant nuclei.

    abstract::We analysed the presence and localization of spectrin-like proteins in nuclei of various plant tissues, using several anti-erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross-reactive pair of bands at 220-240 kDa, typical for human ery...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0508

    authors: De Ruijter NC,Ketelaar T,Blumenthal SS,Emons AM,Schel JH

    更新日期:2000-01-01 00:00:00

  • Peroxynitrite activates K+-Cl- cotransport in human erythrocytes.

    abstract::Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0778

    authors: Grzelak A,Mazur J,Bartosz G

    更新日期:2001-01-01 00:00:00

  • Association of nucleus and centrosome: magnet or velcro?

    abstract::A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10016

    authors: Burakov AV,Nadezhdina ES

    更新日期:2013-02-01 00:00:00

  • Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.

    abstract::Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramm...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10554

    authors: Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J

    更新日期:2016-02-01 00:00:00

  • Verification of epigenetic inheritance in a unicellular model system: multigenerational effects of hormonal imprinting.

    abstract::The unicellular Tetrahymena has receptors for hormones of higher vertebrates, produces these hormones, and their signal pathways are similar. The first encounter with a hormone in higher dose provokes the phenomenon of hormonal imprinting, by which the reaction of the cell is quantitatively modified. This modification...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110677

    authors: Kőhidai L,Lajkó E,Pállinger E,Csaba G

    更新日期:2012-10-01 00:00:00

  • Presence of multiple centrioles and primary cilia during growth and early differentiation in the myoblast CO25 cell line.

    abstract::CO25 cells, a mouse myoblast line, contain multiple centrioles and primary cilia. A most unusual feature has been the finding of large numbers of separate structures in single cells-up to a maximum of nine centrioles, six primary cilia, and 12 of both organelles together. Aberrant multipolar spindles were occasionally...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1996.0103

    authors: Zeytinoglu M,Ritter J,Wheatley DN,Warn RM

    更新日期:1996-12-01 00:00:00

  • Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

    abstract::Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mecha...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10953

    authors: Zhang Y,Zhang Y

    更新日期:2018-07-01 00:00:00

  • MiR-139-5p Upregulation Alleviated Spontaneous Recurrent Epileptiform Discharge-induced Oxidative Stress and Apoptosis in Rat Hippocampal Neurons via Regulating the Notch Pathway.

    abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11509

    authors: Zhao C,Yang F,Wei X,Zhang J

    更新日期:2020-11-28 00:00:00

  • Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells.

    abstract::Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10310

    authors: Pinheiro MD,Bols NC

    更新日期:2014-10-01 00:00:00

  • High-glucose concentration aggravates TNF-alpha-induced cell viability reduction in human CD146-positive periodontal ligament cells via TNFR-1 gene demethylation.

    abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11445

    authors: Luo H,Zhu W,Mo W,Liang M

    更新日期:2020-12-01 00:00:00

  • Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

    abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0343

    authors: Gonzalez CB,Reyes CE,Figueroa CD,Barra V,Troncoso S

    更新日期:1999-01-01 00:00:00

  • Effects of human yolk sac endothelial cells on supporting expansion of hematopoietic stem/progenitor cells from cord blood.

    abstract::In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.06.006

    authors: Hu L,Cheng L,Wang J,Zhao H,Duan H,Lu G

    更新日期:2006-11-01 00:00:00

  • Ultrastructural evidence that apoptosis is the mechanism by which human amylin evokes death in RINm5F pancreatic islet beta-cells.

    abstract::A view is emerging that human amylin (HA) kills pancreatic islet beta-cells by apoptosis. This study strengthens this view by documenting time-dependent morphological and ultrastructural changes in 10 microm HA-treated cultured RINm5F islet beta-cells. Membrane blebbing and microvilli loss were the earliest detectable...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0643

    authors: Saafi EL,Konarkowska B,Zhang S,Kistler J,Cooper GJ

    更新日期:2001-01-01 00:00:00

  • Cytokeratin expression in human respiratory epithelium of nasal polyps and turbinates.

    abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1072

    authors: Hicks W Jr,Ward R,Edelstein D,Hall L 3rd,Albino A,Hard R,Asch B

    更新日期:1995-04-01 00:00:00

  • Erythrocyte sodium/hydrogen exchange inhibition by (-) epicatechin.

    abstract::Epicatechin, a flavonoid belonging to the group of compounds collectively called catechins, have been reported to possess insulin-like properties. Besides their anti-diabetic properties, catechins also show growth inhibition. Since cytosolic pH (pHi) plays a role in cell proliferation and the Na/H exchanger (NHE) is t...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0719

    authors: Matteucci E,Rizvi SI,Giampietro O

    更新日期:2001-01-01 00:00:00

  • TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

    abstract::Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10989

    authors: Li L,Qi Y,Ma X,Xiong G,Wang L,Bao C

    更新日期:2018-09-01 00:00:00

  • Intracellular signal transduction pathways induced by leptin in C2C12 cells.

    abstract::As experimental evidence suggests that leptin may have direct effects on peripheral tissues, we investigated some of the transductional molecules induced by leptin in C2C12 cells. In immunoprecipitation experiments using anti-p85 antibodies (a regulatory subunit of phosphatidylinositol-3-kinase; PI3K), we observed a s...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.03.008

    authors: Maroni P,Bendinelli P,Piccoletti R

    更新日期:2005-07-01 00:00:00

  • Ro52/SSA sensitizes cells to death receptor-induced apoptosis by down-regulating c-FLIP(L).

    abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110322

    authors: Zhang J,Fang L,Zhu X,Qiao Y,Yu M,Wang L,Chen Y,Yin W,Hua ZC

    更新日期:2012-05-01 00:00:00

  • Effects of 3-D clino-rotation on gene expression in human fibroblast cells.

    abstract::Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0833

    authors: Arase Y,Nomura J,Sugaya S,Sugita K,Kita K,Suzuki N

    更新日期:2002-01-01 00:00:00

  • Ectophosphatase activity in the triple-negative breast cancer cell line MDA-MB-231.

    abstract::Breast cancer is one of the most common cancers in the female population worldwide, and its development is thought to be associated with genetic mutations that lead to uncontrolled and accelerated growth of breast cells. This abnormal behavior requires extra energy, and indeed, tumor cells display a rewired energy met...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11497

    authors: Lacerda-Abreu MA,Russo-Abrahão T,Leite Tenório Aguiar R,Monteiro RQ,Rumjanek FD,Meyer-Fernandes JR

    更新日期:2020-11-03 00:00:00

  • Wnt-3 and Wnt-3a play different region-specific roles in neural crest development in avians.

    abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090133

    authors: Dongkyun K,Jinsoo S,Jin EJ

    更新日期:2010-07-01 00:00:00

  • Cytokine profiles, signalling pathways and effects of fluticasone propionate in respiratory syncytial virus-infected human foetal lung fibroblasts.

    abstract::To examine cytokine production in response to RSV infection, we assessed the levels of 29 cytokines released from RSV-infected human foetal lung fibroblasts. We also examined the relationships between the effects of fluticasone propionate and various signalling pathways in the cells. Twenty-four hours after infection ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10044

    authors: Seki E,Yoshizumi M,Tanaka R,Ryo A,Ishioka T,Tsukagoshi H,Kozawa K,Okayama Y,Okabe-Kado J,Goya T,Kimura H

    更新日期:2013-04-01 00:00:00

  • Pigment epithelium-derived factor delays cellular senescence of human mesenchymal stem cells in vitro by reducing oxidative stress.

    abstract::Mesenchymal stem cells (MSCs) are multipotent progenitor cells that represent a promising approach in the field of regenerative medicine; however, this potential diminishes with senescence. Pigment epithelium-derived factor (PEDF) gives some protection by reducing oxidative stress, which is known to accelerate cellula...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10041

    authors: Cao Y,Yang T,Gu C,Yi D

    更新日期:2013-04-01 00:00:00

  • Centrosome overduplication and mitotic instability in PKD2 transgenic lines.

    abstract::Polycystin-2 (PC-2), a protein encoded by PKD2 and involved in autosomal dominant polycystic kidney disease (ADPKD), is a non-selective cationic channel recently implicated in the function of primary cilia. We recently constructed a new animal model in the form of a transgenic mouse with a BAC-containing human PKD2 in...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.07.021

    authors: Burtey S,Riera M,Ribe E,Pennenkamp P,Rance R,Luciani J,Dworniczak B,Mattei MG,Fontés M

    更新日期:2008-10-01 00:00:00

  • New indices in morphometry of nuclear structure in the NIH 3T3 cell line.

    abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00158-6

    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Avagyan VY,Karalova EM

    更新日期:2003-01-01 00:00:00

  • Significance of eIF4E expression in skin squamous cell carcinoma.

    abstract::Cutaneous squamous cell carcinoma (SCC) is a malignant tumour of keratinising epidermal cells. This type of skin cancer is the second leading cause of death after melanoma, and it is the second most common type of non-melanoma skin cancer after basal cell carcinoma. The cellular and molecular events involved in the pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.06.006

    authors: Salehi Z,Mashayekhi F,Shahosseini F

    更新日期:2007-11-01 00:00:00

  • Overexpression of long noncoding RNA HOXA-AS2 predicts an adverse prognosis and promotes tumorigenesis via SOX4/PI3K/AKT pathway in acute myeloid leukemia.

    abstract::Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11370

    authors: Qu Y,Wang Y,Wang P,Lin N,Yan X,Li Y

    更新日期:2020-08-01 00:00:00