Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.


:Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.


Cell Biol Int


Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J




Has Abstract


2016-02-01 00:00:00












  • Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.

    abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kale VP

    更新日期:2020-11-01 00:00:00

  • High-glucose concentration aggravates TNF-alpha-induced cell viability reduction in human CD146-positive periodontal ligament cells via TNFR-1 gene demethylation.

    abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Luo H,Zhu W,Mo W,Liang M

    更新日期:2020-12-01 00:00:00

  • Localization of pan-cadherin immunoreactivity in adult rat tissues.

    abstract::Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Filiz S,Dalcik H,Yardimoglu M,Gonca S,Ceylan S

    更新日期:2002-01-01 00:00:00

  • Stem cells conditioned medium: a new approach to skin wound healing management.

    abstract::Stem cell biology has gained remarkable interest in recent years, driven by the hope of finding cures for numerous diseases including skin wound healing through transplantation medicine. Initially upon transplantation, these cells home to and differentiate within the injured tissue into specialised cells. Contrariwise...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Jayaraman P,Nathan P,Vasanthan P,Musa S,Govindasamy V

    更新日期:2013-10-01 00:00:00

  • Evaluation of STAT3 decoy oligodeoxynucleotides' synergistic effects on radiation and/or chemotherapy in metastatic breast cancer cell line.

    abstract::Resistance to radiotherapy and chemotherapy has been a major problem of conventional cancer therapies, which consequently leads to cancer relapse and cancer-related death. It is known that cancer stem cells (CSCs) play a key role in therapy resistance and CSC-based targeted therapies have been considered as a powerful...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Johari B,Rahmati M,Nasehi L,Mortazavi Y,Faghfoori MH,Rezaeejam H

    更新日期:2020-12-01 00:00:00

  • Possible role of the 'IDO-AhR axis' in maternal-foetal tolerance.

    abstract::The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hao K,Zhou Q,Chen W,Jia W,Zheng J,Kang J,Wang K,Duan T

    更新日期:2013-02-01 00:00:00

  • Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo.

    abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Dubatolova TD,Volkova EI,Omelyanchuk LV

    更新日期:2013-02-01 00:00:00

  • Pigment epithelium-derived factor delays cellular senescence of human mesenchymal stem cells in vitro by reducing oxidative stress.

    abstract::Mesenchymal stem cells (MSCs) are multipotent progenitor cells that represent a promising approach in the field of regenerative medicine; however, this potential diminishes with senescence. Pigment epithelium-derived factor (PEDF) gives some protection by reducing oxidative stress, which is known to accelerate cellula...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cao Y,Yang T,Gu C,Yi D

    更新日期:2013-04-01 00:00:00

  • Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres.

    abstract::The spatial distribution of acid membrane organelles and their relationships with normal and vacuolated transverse tubules has been studied in living frog skeletal muscle fibres using confocal microscopy. Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed t...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Krolenko SA,Adamyan SY,Belyaeva TN,Mozhenok TP

    更新日期:2006-11-01 00:00:00

  • M2 macrophages induce EMT through the TGF-β/Smad2 signaling pathway.

    abstract::IPF is characterized by fibroblast accumulation, collagen deposition, and ECM remodeling, with myofibroblasts believed to be the effector cell type. Myofibroblasts develop due to EMT of lung alveolar epithelial cells, which can be induced by TGF-β. M2 macrophages, a macrophage subpopulation, secrete large amounts of T...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhu L,Fu X,Chen X,Han X,Dong P

    更新日期:2017-09-01 00:00:00

  • Heat shock protein 27 as a neuroprotective biomarker and a suitable target for stem cell therapy and pharmacotherapy in ischemic stroke.

    abstract::Ischemic stroke is a major common cause of death and long-term disability worldwide. Several pathophysiological events including excitotoxicity, oxidative/nitrative stress, inflammation, and apoptosis are involved in ischemic injuries. Recently, the molecular mechanisms involved in cerebral ischemia through a focus on...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Behdarvandy M,Karimian M,Atlasi MA,Azami Tameh A

    更新日期:2020-02-01 00:00:00

  • Combined application of in vivo UV-crosslinking and in vitro label transfer in the examination of AU-rich sequence binding protein-RNA interactions.

    abstract::A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymph...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Henics T,Nagy E,Rigby WF

    更新日期:1995-09-01 00:00:00

  • Mycobacterial growth in human macrophages: variation according to donor, inoculum and bacterial strain.

    abstract::The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PD

    更新日期:2001-01-01 00:00:00

  • CJX1, an amlodipine derivative, interacts with ATPase of human P-glycoprotein.

    abstract::Our aim has been to elucidate the possible mechanism of CJX1, an amlodipine derivative, in the modulation of P-gp function by determining its effect on P-gp ATPase activity. Basal P-gp ATPase activity was increased by CJX1 with half-maximal activity concentration (Km) of 8.6+/-1.4 microM. Kinetic analysis indicated a ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ji BS,He L

    更新日期:2009-10-01 00:00:00

  • Cell death and DNA damage in peritoneal macrophages of mice (Mus musculus) exposed to inorganic lead.

    abstract::Lead is a heavy metal of considerable environmental and occupational concern and there is growing evidence that it is toxic to the human immune system. In this regard, this study examined the effect of lead (Pb) exposure to peritoneal macrophages (Mvarphis) of mice (Mus musculus) cultivated in DMEM medium supplemented...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Gargioni R,Filipak Neto F,Buchi DF,Randi MA,Franco CR,Paludo KS,Pelletier E,Ferraro MV,Cestari MM,Bussolaro D,Oliveira Ribeiro CA

    更新日期:2006-07-01 00:00:00

  • VEGF expression is regulated by HIF-1α and ARNT in 3D KYSE-70, esophageal cancer cell spheroids.

    abstract::In 3D cultured cell systems, the cells form 3D spheroids that mimic cancer cell spheroids in vivo. Cancer cells form cell spheroids as they grow. The in vivo spheroids do not contain a vascular network; therefore, oxygen and nutrition supplies are insufficient. Specifically, the cells in the core region of the cluster...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Terashima J,Sampei S,Iidzuka M,Ohsakama A,Tachikawa C,Satoh J,Kudo K,Habano W,Ozawa S

    更新日期:2016-11-01 00:00:00

  • Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

    abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Andries L,Harrisson F,Vakaet L

    更新日期:1994-07-01 00:00:00

  • Wnt-3 and Wnt-3a play different region-specific roles in neural crest development in avians.

    abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Dongkyun K,Jinsoo S,Jin EJ

    更新日期:2010-07-01 00:00:00

  • Characterization and expression of amphioxus ApoD gene encoding an archetype of vertebrate ApoD proteins.

    abstract::Here we report a homologue of the apolipoprotein D gene (AmphiApoD) in amphioxus, Branchiostoma belcheri tsingtauense, the first such finding in a basal chordate cephalochordate. The main features of the protein predicted from AmphiApoD are characteristic of the apolipoprotein D. Phylogenetic analysis places AmphiApoD...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Wang L,Zhang S,Liu Z,Li H,Wang Y,Jiang S

    更新日期:2007-01-01 00:00:00

  • Muscarinic acetylcholine receptors involved in the regulation of neural stem cell proliferation and differentiation in vitro.

    abstract::Neural stem cells (NSCs) are currently considered powerful candidates for cell therapy in neurodegenerative disorders such as Parkinson's disease. However, it is not known when and how NSCs begin to differentiate functionally. Recent reports suggest that classical neurotransmitters such as acetylcholine (Ach) are invo...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou C,Wen ZX,Shi DM,Xie ZP

    更新日期:2004-01-01 00:00:00

  • Knockdown of ghrelin-O-acyltransferase attenuates colitis through the modulation of inflammatory factors and tight junction proteins in the intestinal epithelium.

    abstract::Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The intestinal mucosal barrier prevents...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Tian P,Lu X,Jin N,Shi J

    更新日期:2020-08-01 00:00:00

  • Effects of 3-D clino-rotation on gene expression in human fibroblast cells.

    abstract::Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Arase Y,Nomura J,Sugaya S,Sugita K,Kita K,Suzuki N

    更新日期:2002-01-01 00:00:00

  • OP9-DL1 cell co-culture enhances anti-tumour immunity of mouse bone marrow-derived dendritic cells.

    abstract::DCs (dendritic cells) are the strongest professional APCs (antigen-presenting cells) to initiate immune responses against pathogens, but they are usually incompetent in initiating efficient immune responses in the progress of solid tumours. We have shown that Notch signalling plays a pivotal role in DC-dependent anti-...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Feng F,Yang J,Tong L,Yuan S,Tian Y,Hong L,Wang W,Zhang H

    更新日期:2012-03-01 00:00:00

  • gp130-specific antisense oligonucleotides inhibit IL-6 signal inducing junB mRNA transcription in the human hepatoma cell line, HepG2.

    abstract::The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus A

    更新日期:2001-01-01 00:00:00

  • The possible double-edged sword effects of vitamin D on COVID-19: A hypothesis.

    abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Shojaeefar E,Malih N,Rezaei N

    更新日期:2021-01-01 00:00:00

  • Sterol glucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica.

    abstract::Mutants of the methanol-utilizing yeast Pichia pastoris and the alkane-utilizing yeast Yarrowia lipolytica defective in the orthologue of UGT51 (encoding sterol glucosyltransferase) were isolated and compared. These mutants do not contain the specific ergosterol derivate, ergosterol glucoside. We observed that the P. ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Stasyk OV,Nazarko TY,Stasyk OG,Krasovska OS,Warnecke D,Nicaud JM,Cregg JM,Sibirny AA

    更新日期:2003-01-01 00:00:00

  • Electrically induced gel-to-gel phase-transition in neurons.

    abstract::At the end of transcardial perfusions with ice-cold physiological saline for 30 min or with isoosmotic potassium chloride for 5 min, but immediately before perfusion fixation, condenser-discharge electric shocks were administered to rats through surface electrodes pressed onto the temporal muscles of the scalped skull...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kellermayer R,Zsombok A,Auer T,Gallyas F

    更新日期:2006-02-01 00:00:00

  • Ectophosphatase activity in the triple-negative breast cancer cell line MDA-MB-231.

    abstract::Breast cancer is one of the most common cancers in the female population worldwide, and its development is thought to be associated with genetic mutations that lead to uncontrolled and accelerated growth of breast cells. This abnormal behavior requires extra energy, and indeed, tumor cells display a rewired energy met...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Lacerda-Abreu MA,Russo-Abrahão T,Leite Tenório Aguiar R,Monteiro RQ,Rumjanek FD,Meyer-Fernandes JR

    更新日期:2020-11-03 00:00:00

  • Enhancement of fertilization by piperine in hamsters.

    abstract::The effect of piperine on the fertilization of eggs with sperm was investigated in female hamsters. They were intragastrically treated with piperine at doses of 50 and 100 mg/kg BW from day 1 through day 4 of the oestrous cycle. During piperine treatment, these females were superovulated and artificially inseminated (...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Piyachaturawat P,Pholpramool C

    更新日期:1997-07-01 00:00:00

  • Mitochondria, endoplasmic reticulum and actin filament behavior after PDT with chloroaluminum phthalocyanine liposomal in HeLa cells.

    abstract::Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Maftoum-Costa M,Naves KT,Oliveira AL,Tedesco AC,da Silva NS,Pacheco-Soares C

    更新日期:2008-08-01 00:00:00