Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.

Abstract:

:Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.

journal_name

Cell Biol Int

authors

Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J

doi

10.1002/cbin.10554

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

214-22

issue

2

eissn

1065-6995

issn

1095-8355

journal_volume

40

pub_type

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