Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.


:Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.


Cell Biol Int


Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J




Has Abstract


2016-02-01 00:00:00












  • Suppression of ATP-induced Cl(-)secretion by enhanced expression of epithelial Na(+)channels in mouse endometrial epithelium.

    abstract::We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Wang XF,Tsang LL,So SC,Chan HC

    更新日期:2001-01-01 00:00:00

  • LncRNA MAFG-AS1 facilitates the migration and invasion of NSCLC cell via sponging miR-339-5p from MMP15.

    abstract::Non-small-cell carcinoma (NSCLC) is the most common cancer along with high mortality rate worldwide. In the present study, our data showed that lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAFG-AS1) was over-expressed in NSCLC tissues and cell lines. Overexpression of MAFG-AS1 promoted the migration, invasi...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Jia YC,Wang JY,Liu YY,Li B,Guo H,Zang AM

    更新日期:2019-04-01 00:00:00

  • Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

    abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha A

    更新日期:2007-03-01 00:00:00

  • Comparative study of hematopoietic stem and progenitor cells between sexes in mice under physiological conditions along time.

    abstract::Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid pro...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Gasco S,Rando A,Zaragoza P,García-Redondo A,Calvo AC,Osta R

    更新日期:2017-12-01 00:00:00

  • Smart mechanosensing machineries enable migration of vascular smooth muscle cells in atherosclerosis-relevant 3D matrices.

    abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Chi Q,Shan J,Ding X,Yin T,Wang Y,Jia D,Wang G

    更新日期:2017-06-01 00:00:00

  • Comparison of the endogenous IK currents in rat hippocampal neurons and cloned Kv2.1 channels in CHO cells.

    abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Liu M,Gong B,Qi Z

    更新日期:2008-12-01 00:00:00

  • Pioglitazone suppresses the lipopolysaccharide-induced production of inflammatory factors in mouse macrophages by inactivating NF-kappaB.

    abstract::TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ao C,Huo Y,Qi L,Xiong Z,Xue L,Qi Y

    更新日期:2010-07-01 00:00:00

  • Verification of epigenetic inheritance in a unicellular model system: multigenerational effects of hormonal imprinting.

    abstract::The unicellular Tetrahymena has receptors for hormones of higher vertebrates, produces these hormones, and their signal pathways are similar. The first encounter with a hormone in higher dose provokes the phenomenon of hormonal imprinting, by which the reaction of the cell is quantitatively modified. This modification...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kőhidai L,Lajkó E,Pállinger E,Csaba G

    更新日期:2012-10-01 00:00:00

  • Microbiological contamination in stem cell cultures.

    abstract::Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential f...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cobo F,Cortés JL,Cabrera C,Nieto A,Concha A

    更新日期:2007-09-01 00:00:00

  • Rapamycin improves insulin resistance and hepatic steatosis in type 2 diabetes rats through activation of autophagy.

    abstract::Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou W,Ye S

    更新日期:2018-09-01 00:00:00

  • Morphometry of nuclear and nucleolar structures in a CaCo-2 cell line.

    abstract::The number of the nucleoli in a CaCo-2 cell nucleus does not generally depend on the quantity of DNA in the nucleus, but nucleolar DNA content is directly proportional to total nuclear DNA. However, in multinucleolar cells (three or more nucleoli), the nucleolar DNA content increases after 96 h incubation in culture w...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Karalova EM

    更新日期:2004-01-01 00:00:00

  • Apoptotic effect of eugenol in human colon cancer cell lines.

    abstract::Eugenol, a natural compound available in honey and various plants extracts including cloves and Magnoliae flos, is exploited for various medicinal applications. Since most of the drugs used in the cancer are apoptotic inducers, the apoptotic effect and anticancer mechanism of eugenol were investigated against colon ca...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Jaganathan SK,Mazumdar A,Mondhe D,Mandal M

    更新日期:2011-06-01 00:00:00

  • Combined application of in vivo UV-crosslinking and in vitro label transfer in the examination of AU-rich sequence binding protein-RNA interactions.

    abstract::A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymph...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Henics T,Nagy E,Rigby WF

    更新日期:1995-09-01 00:00:00

  • Decreased intracellular proteolysis correlates with the maintenance of a specific isoenzyme of cytochrome P-450.

    abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Evans PJ

    更新日期:1999-01-01 00:00:00

  • Effects of spaceflight conditions on fertilization and embryogenesis in the sea urchin Lytechinus pictus.

    abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Schatten H,Chakrabarti A,Taylor M,Sommer L,Levine H,Anderson K,Runco M,Kemp R

    更新日期:1999-01-01 00:00:00

  • Modulation of CYP3A4 activity and induction of apoptosis, necrosis and senescence by the anti-tumour imidazoacridinone C-1311 in human hepatoma cells.

    abstract::There is increasing evidence that the expression level of drug metabolic enzymes affects the final cellular response following drug treatment. Moreover, anti-tumour agents may modulate enzymatic activity and/or cellular expression of metabolic enzymes in tumour cells. We have investigated the influence of CYP3A4 overe...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Augustin E,Pawłowska M,Polewska J,Potega A,Mazerska Z

    更新日期:2013-02-01 00:00:00

  • Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy.

    abstract::Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: ma...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ortega-Martínez M,Gutiérrez-Dávila V,Niderhauser-García A,Salazar-Aranda R,Solís-Soto JM,Montes-de-Oca-Luna R,Jaramillo-Rangel G

    更新日期:2020-03-01 00:00:00

  • Ro52/SSA sensitizes cells to death receptor-induced apoptosis by down-regulating c-FLIP(L).

    abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhang J,Fang L,Zhu X,Qiao Y,Yu M,Wang L,Chen Y,Yin W,Hua ZC

    更新日期:2012-05-01 00:00:00

  • The suppressive effect of CD25+Treg cells on Th1 differentiation requires cell-cell contact partially via TGF-β production.

    abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Shen E,Zhao K,Wu C,Yang B

    更新日期:2011-07-01 00:00:00

  • Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling.

    abstract::Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

    更新日期:2020-02-01 00:00:00

  • Fractionation analysis of the endosomal compartment during rat reticulocyte maturation.

    abstract::A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Dardalhon V,Géminard C,Reggio H,Vidal M,Sainte-Marie J

    更新日期:2002-01-01 00:00:00

  • The significance of alpha 5 beta 1 integrin dependent and independent actin cytoskelton organization in cell transformation and survival.

    abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Rajeswari J,Pande G

    更新日期:2002-01-01 00:00:00

  • The DNA binding affinity of rat liver nucleoproteins to the regulatory elements of the haptoglobin and alpha 2-macroglobulin genes.

    abstract::Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2MG) genes was examined in the livers of rats during the acute-phase reaction. Our results demonstrate that the elevation of the Hp and...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Petrović M,Bogojević D,Grigorov I,Sevaljević L

    更新日期:1995-12-01 00:00:00

  • Metabolic profiling of normal hepatocyte and hepatocellular carcinoma cells via 1 H nuclear magnetic resonance spectroscopy.

    abstract::Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Multiv...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Chen Y,Chen Z,Feng JH,Chen YB,Liao NS,Su Y,Zou CY

    更新日期:2018-04-01 00:00:00

  • Human endometrial stem cells differentiation into functional hepatocyte-like cells.

    abstract::In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purificatio...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Khademi F,Soleimani M,Verdi J,Tavangar SM,Sadroddiny E,Massumi M,Ai J

    更新日期:2014-07-01 00:00:00

  • Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line.

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    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cheng SC,Luo D,Xie Y

    更新日期:2001-01-01 00:00:00

  • Nitric oxide induced oxidative changes in erythrocyte membrane components.

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    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Brzeszczynska J,Gwozdzinski K

    更新日期:2008-01-01 00:00:00

  • miR-483-3p plays an oncogenic role in esophageal squamous cell carcinoma by targeting tumor suppressor EI24.

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    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ma J,Hong L,Xu G,Hao J,Wang R,Guo H,Liu J,Zhang Y,Nie Y,Fan D

    更新日期:2016-04-01 00:00:00

  • Establishment and characterization of endothelial cell lines from the aorta of miniature pig for the study of xenotransplantation.

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    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kim D,Kim JY,Koh HS,Lee JP,Kim YT,Kang HJ,Hwang WS,Kim YB,Lee JS,Ahn C

    更新日期:2005-08-01 00:00:00

  • vasa-related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala).

    abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Shukalyuk AI,Golovnina KA,Baiborodin SI,Gunbin KV,Blinov AG,Isaeva VV

    更新日期:2007-02-01 00:00:00