Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei.

Abstract:

:Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.

journal_name

Cell Biol Int

authors

Fu B,Liu D,Ma H,Guo ZH,Wang L,Li ZQ,Peng FG,Bai J

doi

10.1002/cbin.10554

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

214-22

issue

2

eissn

1065-6995

issn

1095-8355

journal_volume

40

pub_type

杂志文章
  • Differentiation of embryonic stem cells towards pancreatic progenitor cells and their transplantation into streptozotocin-induced diabetic mice.

    abstract::Type I diabetes is characterized by the deficiency of endocrine beta cells in the pancreatic islets of Langerhans and transplantation of islet cells can be an effective therapeutic approach. Embryonic stem cells can be differentiated into any cell type, and therefore represent an unlimited source of islet cells for th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.12.017

    authors: Chen C,Zhang Y,Sheng X,Huang C,Zang YQ

    更新日期:2008-04-01 00:00:00

  • Dendritic cell uptake of iron-based magnetic nanoparticles.

    abstract::We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no e...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.04.001

    authors: Goya GF,Marcos-Campos I,Fernández-Pacheco R,Sáez B,Godino J,Asín L,Lambea J,Tabuenca P,Mayordomo JI,Larrad L,Ibarra MR,Tres A

    更新日期:2008-08-01 00:00:00

  • Effects of adenosine and epidermal growth factor on the growth of mouse mammary epithelial cells.

    abstract::The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine at concentrations o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0231

    authors: Yuh IS,Jang BB,Hong BJ

    更新日期:1998-01-01 00:00:00

  • Fractionation analysis of the endosomal compartment during rat reticulocyte maturation.

    abstract::A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0917

    authors: Dardalhon V,Géminard C,Reggio H,Vidal M,Sainte-Marie J

    更新日期:2002-01-01 00:00:00

  • Intracellular localization of Xenopus small heat shock protein, hsp30, in A6 kidney epithelial cells.

    abstract::Small heat shock proteins (shsps) are molecular chaperones that are inducible by environmental stress. In this study, immunocytochemical analysis and laser scanning confocal microscopy revealed that the shsp family, hsp30, was localized primarily in the cytoplasm of Xenopus A6 kidney epithelial cells after heat shock ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.12.006

    authors: Gellalchew M,Heikkila JJ

    更新日期:2005-03-01 00:00:00

  • Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase.

    abstract::ROS (reactive oxygen species) take an important signalling role in angiogenesis. Although there are several ways to produce ROS in cells, multicomponent non-phagocytic NADPH oxidase is an important source of ROS that contribute to angiogenesis. In the present work, we examined the effects of H2O2 on angiogenesis inclu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090092

    authors: Mu P,Liu Q,Zheng R

    更新日期:2010-10-01 00:00:00

  • Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.

    abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.04.003

    authors: Elinos-Báez CM,León F,Santos E

    更新日期:2005-08-01 00:00:00

  • Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα-knockdown cells.

    abstract::Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-rela...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10902

    authors: Yang D,Okamura H,Qiu L

    更新日期:2018-04-01 00:00:00

  • Runx2 alleviates high glucose-suppressed osteogenic differentiation via PI3K/AKT/GSK3β/β-catenin pathway.

    abstract::Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs)...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10779

    authors: Chen Y,Hu Y,Yang L,Zhou J,Tang Y,Zheng L,Qin P

    更新日期:2017-08-01 00:00:00

  • WITHDRAWN: Proteinase activated receptor-2 mediated IL-5, IL-6 and RANTES release from human eosinophils.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.01.011

    authors: Wang H,Huang Y

    更新日期:2008-01-25 00:00:00

  • Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.

    abstract::The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0207

    authors: Yamamoto M,Aoyagi M,Akazawa K,Tajima S,Yamamoto K

    更新日期:1998-01-01 00:00:00

  • Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3.

    abstract::The purpose of this study was three fold: (1) to reveal the implications of Wnt5A for cytokine and chemokine production by human ovarian cancer cell line SKOV-3 cells, (2) to determine the influence of Wnt5A on chemotactic SKOV-3 cell migration, and (3) to assess the effect of inflammatory mediators on Wnt5A expressio...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10551

    authors: Arabzadeh S,Hossein G,Zarnani AH

    更新日期:2016-02-01 00:00:00

  • Keratins and skin disorders.

    abstract::The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:

    authors: Rao KS,Babu KK,Gupta PD

    更新日期:1996-04-01 00:00:00

  • New indices in morphometry of nuclear structure in the NIH 3T3 cell line.

    abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00158-6

    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Avagyan VY,Karalova EM

    更新日期:2003-01-01 00:00:00

  • Cytoskeleton during aerenchyma formation in plants.

    abstract::Aerenchyma is a plant tissue characterized by prominent intercellular spaces facilitating gas diffusion between roots and the aerial environment. The classical formation of intercellular spaces is thought to be the result of schizogeny and lysogeny during development of wetland species and in some dry-land species in ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10814

    authors: Kordyum EL,Shevchenko GV,Brykov VO

    更新日期:2019-09-01 00:00:00

  • Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena.

    abstract::FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.06.019

    authors: Csaba G,Kovács P,Tóthfalusi L,Pállinger E

    更新日期:2006-12-01 00:00:00

  • Role of phosphorylation of the cytoplasmic domain of the alpha(2)-macroglobulin receptor (LRP).

    abstract::The low density lipoprotein receptor-related protein (alpha(2)MR/LRP) is a cell surface receptor which is present on most cells and tissues. We show that the 85 kDa subunit, containing the transmembrane region and cytoplasmic domain is phosphorylated in vivo. Comparison of the phosphorylation of the low density lipopr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0561

    authors: Djordjevic JT,Waterkeyn JG,Hennessy KL,Stanley KK

    更新日期:2000-01-01 00:00:00

  • The effect of oxidative stress induced by t-butyl hydroperoxide on the structural dynamics of membrane proteins of Chinese hamster fibroblasts.

    abstract::A method based on the measurement at room temperature of tryptophan phosphorescence (RTTP) gives the unique possibility to investigate the dynamic structure of membrane proteins without their isolation from cells. This method was used to study the influence of tert- butyl hydroperoxide (t -BHP) on Chinese hamster fibr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0366

    authors: Mazhul V,Shcherbin D,Zavodnik I,Rekawiecka K,Bryszewska M

    更新日期:1999-01-01 00:00:00

  • Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia.

    abstract::Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2003.12.004

    authors: Dalby MJ,Riehle MO,Johnstone H,Affrossman S,Curtis AS

    更新日期:2004-01-01 00:00:00

  • Aphidicolin inhibits DNA replication in the micronucleus and blocks cytokinesis in Paramecium caudatum.

    abstract::Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0482

    authors: Sabaneyeva E,Tao W,Verbelen JP

    更新日期:1999-01-01 00:00:00

  • The level and phosphorylation of Hsp70 in the rat liver cytosol after adrenalectomy and hyperthermia.

    abstract::Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 degrees C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0247

    authors: Cvoro A,Dundjerski J,Trajković D,Matić G

    更新日期:1999-01-01 00:00:00

  • In vitro-induced differentiation of bone marrow mesenchymal stem cells into melanocytes.

    abstract::Large numbers of autogenous melanocytes (Mcs) are required when conducting studies on tissue engineering of skin and performing surgical treatment of depigmentation diseases. This study was conducted to explore the possibility of inducing differentiation of bone marrow mesenchymal stem cells (MSCs) into Mcs as a means...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10455

    authors: Mei X,Sun Y,Wu Z,Pan W,Zhu J,Shi W

    更新日期:2015-07-01 00:00:00

  • Smart mechanosensing machineries enable migration of vascular smooth muscle cells in atherosclerosis-relevant 3D matrices.

    abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10764

    authors: Chi Q,Shan J,Ding X,Yin T,Wang Y,Jia D,Wang G

    更新日期:2017-06-01 00:00:00

  • Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

    abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0343

    authors: Gonzalez CB,Reyes CE,Figueroa CD,Barra V,Troncoso S

    更新日期:1999-01-01 00:00:00

  • Localization of pan-cadherin immunoreactivity in adult rat tissues.

    abstract::Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0956

    authors: Filiz S,Dalcik H,Yardimoglu M,Gonca S,Ceylan S

    更新日期:2002-01-01 00:00:00

  • Erythropoietin enhances mitochondrial biogenesis in cardiomyocytes exposed to chronic hypoxia through Akt/eNOS signalling pathway.

    abstract::Adaptation of cardiomyocytes to chronic hypoxia in cyanotic patients remains unclear. Mitochondrial biogenesis is enhanced in myocardium from cyanotic patients, which is possibly an adaptive response. Erythropoietin (EPO) in blood and its receptor (EPOR) on cardiomyocytes are upregulated by chronic hypoxia, suggesting...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10205

    authors: Qin C,Zhou S,Xiao Y,Chen L

    更新日期:2014-03-01 00:00:00

  • Effect of monochromatic visible light on intracellular superoxide anion production and mitochondrial membrane potential of B16F1 and B16F10 murine melanoma cells.

    abstract::We have investigated the effect of visible light on animal cells using light-emitting diodes to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10069

    authors: Sato K,Minai Y,Watanabe H

    更新日期:2013-06-01 00:00:00

  • ABCC9, NKAPL, and TMEM132C are potential diagnostic and prognostic markers in triple-negative breast cancer.

    abstract::Triple-negative breast cancer (TNBC) is a highly heterogeneous disease. The aim of this study is to identify the diagnostic and poor prognostic signatures in TNBC by exploring the aberrant DNA methylation and gene expression. Differential expression and methylation analysis of the TNBC and paracancer samples from The ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11406

    authors: Zhang X,Kang X,Jin L,Bai J,Zhang H,Liu W,Wang Z

    更新日期:2020-10-01 00:00:00

  • Nuclear antigens, as DNA, of DSB389 MAb and some other anti-desmin monoclonal antibodies.

    abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1996.0099

    authors: Kamei H

    更新日期:1996-11-01 00:00:00

  • Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

    abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10380

    authors: Wan Q,Lu H,Wu LT,Liu X,Xiang JB

    更新日期:2014-12-01 00:00:00