Runx2 alleviates high glucose-suppressed osteogenic differentiation via PI3K/AKT/GSK3β/β-catenin pathway.


:Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs) in high-glucose condition. In this study, the effect of Runx2 on osteoblast differentiation in high-glucose condition was evaluated by the expression of osteogenesis-related maker including Runx2, ALP, OC, and OPN, as well as ALP staining, ALP activity, and Alizarin red S staining. Western blot analysis was performed to detect the protein expression levels of p-AKT, AKT, p-GSK3β, GSK3β, and β-catenin. Immunofluorescence staining analysis was performed to detect subcellular localization of β-catenin. Our results revealed that high glucose significantly inhibited osteogenic differentiation, hyperosmolarity did not cause a suppression. In addition, Runx2 could upregulate the expression of osteogenic-related genes and increase matrix mineralization, while applying 10 µM PI3K/AKT inhibitor LY294002 abolished the beneficial effect. Collectively, these results indicate that Runx2 alleviates high glucose-induced inhibition of osteoblast differentiation by modulating PI3K/AKT/GSK3β/β-catenin pathway.


Cell Biol Int


Chen Y,Hu Y,Yang L,Zhou J,Tang Y,Zheng L,Qin P




Has Abstract


2017-08-01 00:00:00












  • vasa-related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala).

    abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Shukalyuk AI,Golovnina KA,Baiborodin SI,Gunbin KV,Blinov AG,Isaeva VV

    更新日期:2007-02-01 00:00:00

  • Protein domains involved in nuclear transport of Fos.

    abstract::The protein encoded by the proto-oncogene c-fos is constitutively nuclear in most cell types analyzed. It has a predicted molecular weight of about 55 kDa. Proteins with a molecular weight above 40 kDa cannot enter the nucleus passively. Our interest was to study which regions in the protein are involved in the nuclea...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Campos MA,Kroon EG,Gentz R,Ferreira PC

    更新日期:1999-01-01 00:00:00

  • Effect of metallothionein on cell viability and its interactions with cadmium and zinc in HEK293 cells.

    abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Li J,Liu Y,Ru B

    更新日期:2005-10-01 00:00:00

  • Staurosporine-induced morphological changes in the rat osteoblasts.

    abstract::Treatment of cultured rat osteoblasts with staurosporine caused a rapid outgrowth of long slender cellular processes and the formation of stellate cells. The number of stellate cells increased with higher concentrations of and longer incubation with staurosporine. Scanning electron microscopy (SEM) revealed that the s...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Yang RS,Lu KS,Fu WM,Liu TK,Lin-Shiau SY

    更新日期:1993-01-01 00:00:00

  • Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model.

    abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Chen G,Tao Q

    更新日期:2019-02-01 00:00:00

  • Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees.

    abstract::The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Patricio K,Calábria LK,Peixoto PM,Espindola FS,Da Cruz-Landim C

    更新日期:2010-10-01 00:00:00

  • Acylase 1 expression in rat intestinal crypt--villus axis.

    abstract::Acylase 1 was investigated at the cellular level in the rat small intestine along the enterocyte-differentiation axis. As confirmed by microscopic analysis, villus tip cells and crypt cells of rat jejunal mucosa were successfully separated using the Weiser method. The proliferating undifferentiated crypt cells showed ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cigna N,Nicoletti C,Durand A,Chaix JC,Giardina T,Perrier J

    更新日期:2007-09-01 00:00:00

  • Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo.

    abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Dubatolova TD,Volkova EI,Omelyanchuk LV

    更新日期:2013-02-01 00:00:00

  • Chromatin fibers: from classical descriptions to modern interpretation.

    abstract::The first description of intrachromosomal fibers was made by Baranetzky in 1880. Since that time, a plethora of fibrillar substructures have been described inside the mitotic chromosomes, and published data indicate that chromosomes may be formed as a result of the hierarchical folding of chromatin fibers. In this rev...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Kuznetsova MA,Sheval EV

    更新日期:2016-11-01 00:00:00

  • JQ1, a BET-bromodomain inhibitor, inhibits human cancer growth and suppresses PD-L1 expression.

    abstract::Most traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule-targeted drugs. Programmed death-ligand 1 (PD-L1) is associated with the prognosis of several cancer types, and blockade of PD-1/PD-L1 signaling increases the amplitude o...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Liu K,Zhou Z,Gao H,Yang F,Qian Y,Jin H,Guo Y,Liu Y,Li H,Zhang C,Guo J,Wan Y,Chen R

    更新日期:2019-06-01 00:00:00

  • Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

    abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Gonzalez CB,Reyes CE,Figueroa CD,Barra V,Troncoso S

    更新日期:1999-01-01 00:00:00

  • Pigment epithelium-derived factor delays cellular senescence of human mesenchymal stem cells in vitro by reducing oxidative stress.

    abstract::Mesenchymal stem cells (MSCs) are multipotent progenitor cells that represent a promising approach in the field of regenerative medicine; however, this potential diminishes with senescence. Pigment epithelium-derived factor (PEDF) gives some protection by reducing oxidative stress, which is known to accelerate cellula...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cao Y,Yang T,Gu C,Yi D

    更新日期:2013-04-01 00:00:00

  • Possible role of the 'IDO-AhR axis' in maternal-foetal tolerance.

    abstract::The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hao K,Zhou Q,Chen W,Jia W,Zheng J,Kang J,Wang K,Duan T

    更新日期:2013-02-01 00:00:00

  • Tertiary structure of the hepatic cell protein fibrinogen in fluid revealed by atomic force microscopy.

    abstract::Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Taatjes DJ,Quinn AS,Jenny RJ,Hale P,Bovill EG,McDonagh J

    更新日期:1997-11-01 00:00:00

  • Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

    abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha A

    更新日期:2007-03-01 00:00:00

  • Stimulation of the side population fraction of ATDC5 chondroprogenitors by hypoxia.

    abstract::The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transport...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kishimoto KN,Oxford CL,Reddi AH

    更新日期:2009-12-01 00:00:00

  • The DNA binding affinity of rat liver nucleoproteins to the regulatory elements of the haptoglobin and alpha 2-macroglobulin genes.

    abstract::Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2MG) genes was examined in the livers of rats during the acute-phase reaction. Our results demonstrate that the elevation of the Hp and...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Petrović M,Bogojević D,Grigorov I,Sevaljević L

    更新日期:1995-12-01 00:00:00

  • Inhibition of zoledronic acid derivatives with extended methylene linkers on osteoclastogenesis involve downregulation of JNK and Akt pathways.

    abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Peng Y,Liu Q,Xu D,Li K,Li H,Qiu L,Lin J

    更新日期:2021-01-06 00:00:00

  • YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells.

    abstract::Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio-informatics prediction, forkhead box P4 anti...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Li Y,Li T,Yang Y,Kang W,Dong S,Cheng S

    更新日期:2020-07-01 00:00:00

  • Decrease in p53 protein in cultured cardinal ligament fibroblasts from patients with prolapsus uteri.

    abstract::The authors examined the growth response of cardinal ligamental fibroblasts derived from patients with prolapsus uteri (HPLiF) and compared it with the response of those from control subjects (HCLiF). The growth rate during the logarithmic growth phase was not different between HPLiF and HCLiF, while the cell density ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Yamamoto M,Aoyagi M,Akazawa K,Tajima S,Yamamoto K

    更新日期:1998-01-01 00:00:00

  • Heat shock protein 27 expression in the human testis showing normal and abnormal spermatogenesis.

    abstract::Heat shock proteins (HSPs) are molecular chaperones involved in protein folding, assembly and transport, and which play critical roles in the regulation of cell growth, survival and differentiation. We set out to test the hypothesis that HSP27 protein is expressed in the human testes and its expression varies with the...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Adly MA,Assaf HA,Hussein MR

    更新日期:2008-10-01 00:00:00

  • Reduction of extracellular superoxide dismutase activity by decapeptide derived from FGF-receptor.

    abstract::Several synthetic decapeptides containing an HAV tripeptide motif were tested for their ability to modulate the enzymatic activity of rat extracellular SOD, an enzyme which also contains an HAV motif. Out of nine decapeptides that were tested, only a FGF-receptor derived peptide was active as a negative modulator of e...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Willems J,Noppe W,Slegers H

    更新日期:1995-11-01 00:00:00

  • Increased levels of UV-induced protease activity in human UVAP-1 cells exposed to gravity-changing stress: involvement of E-64-sensitive proteases in suppression of UV mutagenicity.

    abstract::Under the 1G condition, the increase in antipain-sensitive protease activity promptly after UV (mainly 254 nm wavelength) irradiation in cultured human cells is detected and found to be one of the intriguing events involved in suppression of cell mutability. It was found that two cell lines, RSa and its variant UVAP-1...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Takahashi S,Zhang HC,Hasegawa R,Karata K,Kita K,Wano C,Yamaguchi Y,Sugaya S,Nomura J,Ichinose M,Suzuki N

    更新日期:2003-01-01 00:00:00

  • Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5.

    abstract::The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence inclu...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Nie YW,Ding XB,Ge XG,Fan HL,Liu ZW,Guo H

    更新日期:2013-09-01 00:00:00

  • Smart mechanosensing machineries enable migration of vascular smooth muscle cells in atherosclerosis-relevant 3D matrices.

    abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Chi Q,Shan J,Ding X,Yin T,Wang Y,Jia D,Wang G

    更新日期:2017-06-01 00:00:00

  • The influence of dose fractionation on radiation-induced haemolysis of human erythrocytes.

    abstract::In this paper the radioresistance of human erythrocytes to radiation-induced haemolysis was studied by means of the split-dose technique. Human erythrocytes suspended in an isotonic Na-phosphatase buffer, pH 7.4 were exposed under air to gamma radiation. Erythrocytes were irradiated with a single dose of 2.7 kGy and w...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zaborowski A,Szweda-Lewandowska Z

    更新日期:1997-09-01 00:00:00

  • Na+/H+ exchanger 1 inhibition contributes to K562 leukaemic cell differentiation.

    abstract::The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and ma...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Jin W,Li Q,Wang J,Chang G,Lin Y,Li H,Wang L,Gao W,Pang T

    更新日期:2012-08-01 00:00:00

  • Articular chondrocyte passage number: influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography.

    abstract::The isolation and culture of articular chondrocytes is a prerequisite of their use in tissue engineering, but prolonged culture and passaging is associated with de-differentiation. In this paper we studied the influence of nanometric and micrometric grooves (85 nm to 8 microm in depth and 2 microm to 20 microm in widt...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hamilton DW,Riehle MO,Monaghan W,Curtis AS

    更新日期:2005-06-01 00:00:00

  • New indices in morphometry of nuclear structure in the NIH 3T3 cell line.

    abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Avagyan VY,Karalova EM

    更新日期:2003-01-01 00:00:00

  • Single amino acid mutations in the Saccharomyces cerevisiae rhomboid peptidase, Pcp1p, alter mitochondrial morphology.

    abstract::Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Huddleston ME,Xiao N,Both AP,Gordon DM

    更新日期:2019-08-23 00:00:00