Abstract:
:The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were searched for in an IFs-deficient clone of SW13 cells. Reactive materials specific to DSB389, AC54, and DSB860 MAbs were trapped at the top of the gel of SDS-agarose-PAGE. The reactivity of the materials disappeared after treatment with DNase I. The reactivity, or trapping of this material at the top of the gel, required previous heat treatment of the sample before application to the gel. The MAbs recognized both single-stranded and double-stranded DNA in ELISA. These results indicate that at least the main nuclear antigens of DSB389, DSB860 and AC54 MAbs are DNA.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Kamei Hdoi
10.1006/cbir.1996.0099subject
Has Abstractpub_date
1996-11-01 00:00:00pages
769-75issue
11eissn
1065-6995issn
1095-8355pii
S1065-6995(96)90099-2journal_volume
20pub_type
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