Modification of the alkaline comet assay with human mesenchymal stem cells.

abstract：:FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...

journal_title：Cell biology international

authors： Guo J,Bian Y,Wang Y,Chen L,Yu A,Sun X

更新日期：2017-10-01 00:00:00

abstract：:Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

journal_title：Cell biology international

authors： Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

更新日期：2015-02-01 00:00:00

abstract：:Previous trials performed in our laboratory have demonstrated that stainless steel corrosion products induced morphological changes in mice seminiferous cells. In this study the functional nature of the blood-testis barrier was investigated, in view of the extensive use of this metallic alloy in orthopaedic surgery. T...

journal_title：Cell biology international

authors： Pereira ML

更新日期：1995-07-01 00:00:00

abstract：:Prolapsus uteri in pelvic supportive disorders are common in elderly women, and their etiology remains unclear. We examined elastin-binding proteins (EBPs) and binding sites in cultured cardinal ligament fibroblasts derived from elderly patients with prolapsus uteri (HPLiF) and compared them with those from age-matche...

journal_title：Cell biology international

authors： Yamamoto M,Akazawa K,Aoyagi M,Yamamoto N,Yamamoto K

更新日期：2002-01-01 00:00:00

abstract：:The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III incre...

journal_title：Cell biology international

authors： Cipriani C,Pascarella S,Errante F,Menicacci B,Magnelli L,Mocali A,Rovero P,Giovannelli L

更新日期：2018-09-01 00:00:00

abstract：:Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs)...

journal_title：Cell biology international

authors： Chen Y,Hu Y,Yang L,Zhou J,Tang Y,Zheng L,Qin P

更新日期：2017-08-01 00:00:00

abstract：:Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuc...

journal_title：Cell biology international

authors： Liu Q,Guan L,Huang B,Li W,Su Q,Yu M,Xu X,Luo T,Lin S,Sun X,Chen M,Chen X

更新日期：2011-06-01 00:00:00

abstract：:We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The meth...

journal_title：Cell biology international

authors： Forer A,Pickett-Heaps J

更新日期：2005-09-01 00:00:00

abstract：:A plant transformation and selection system has been developed utilizing a modified tubulin gene as a selectable marker. The vector constructs carrying a mutant alpha-tubulin gene from goosegrass conferring resistance to dinitroaniline herbicides were created for transformation of monocotyledonous and dicotyledonous p...

journal_title：Cell biology international

authors： Yemets A,Radchuk V,Bayer O,Bayer G,Pakhomov A,Vance Baird W,Blume YB

更新日期：2008-05-01 00:00:00

abstract：:In the adult brain, neural stem cells from the subgranular zone (SGZ) of the hippocampus and the subventricular zone (SVZ) of the cortex progress through the following five developmental stages: radial glia-like cells, neural progenitor cells, neuroblasts, immature neurons, and mature neurons. These developmental stag...

journal_title：Cell biology international

authors： Jia S,Mou C,Ma Y,Han R,Li X

更新日期：2016-04-01 00:00:00

abstract：:vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...

journal_title：Cell biology international

authors： Shukalyuk AI,Golovnina KA,Baiborodin SI,Gunbin KV,Blinov AG,Isaeva VV

更新日期：2007-02-01 00:00:00

abstract：:The aim of this study was to determine the polarization of macrophages in the tumor microenvironment, as well as the effect of soluble factors secreted from these polarized macrophages on etoposide-induced cancer cell apoptosis. We investigated the effect of soluble factors secreted from the supernatant of PC3 cells t...

journal_title：Cell biology international

authors： Malekghasemi S,Majidi J,Baradaran B,Aghebati-Maleki L

更新日期：2020-10-01 00:00:00

abstract：:Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...

journal_title：Cell biology international

authors： Rajeswari J,Pande G

更新日期：2002-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) play an increasingly important role in cancer growth by coordinately suppressing genes that control cell migration, proliferation, and invasion. The above results can be achieved through the regulation of gene expression by miRNAs by suppressing translation or the direct sequence-specific degradatio...

journal_title：Cell biology international

authors： Sun M,Wang X,Tu C,Wang S,Qu J,Xiao S

更新日期：2017-12-01 00:00:00

abstract：:The effects of NO in its environment may vary considerably depending on various factors. This study shows oxidative mechanism of cellular membrane alterations, which is not associated with triggering of ONOOH generation but is induced by pure NO. Our investigation examined the influence of low concentration of NO (0.1...

journal_title：Cell biology international

authors： Brzeszczynska J,Gwozdzinski K

更新日期：2008-01-01 00:00:00

abstract：:Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...

journal_title：Cell biology international

authors： Cheng SC,Luo D,Xie Y

更新日期：2001-01-01 00:00:00

abstract：:A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymph...

journal_title：Cell biology international

authors： Henics T,Nagy E,Rigby WF

更新日期：1995-09-01 00:00:00

abstract：:Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

journal_title：Cell biology international

authors： Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

更新日期：2020-02-01 00:00:00

abstract：:To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...

journal_title：Cell biology international

authors： Sawhney RS

更新日期：2005-02-01 00:00:00

abstract：:Acylase 1 was investigated at the cellular level in the rat small intestine along the enterocyte-differentiation axis. As confirmed by microscopic analysis, villus tip cells and crypt cells of rat jejunal mucosa were successfully separated using the Weiser method. The proliferating undifferentiated crypt cells showed ...

journal_title：Cell biology international

authors： Cigna N,Nicoletti C,Durand A,Chaix JC,Giardina T,Perrier J

更新日期：2007-09-01 00:00:00

abstract：:Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the under...

journal_title：Cell biology international

authors： Yang J,Shao X,Jiang J,Sun Y,Wang L,Sun L

更新日期：2018-07-01 00:00:00

abstract：:Granulation tissue fibroblasts (myofibroblasts) develop several ultrastructural and biochemical features of smooth muscle (SM) cells, including the presence of microfilaments bundles and the expression of alpha-SM actin, the actin isoform typical of contractile vascular SM cells. Myofibroblasts have been suggested to ...

journal_title：Cell biology international

authors： Desmoulière A

更新日期：1995-05-01 00:00:00

abstract：:The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...

journal_title：Cell biology international

authors： Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PD

更新日期：2001-01-01 00:00:00

abstract：:Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...

journal_title：Cell biology international

authors： Taatjes DJ,Quinn AS,Jenny RJ,Hale P,Bovill EG,McDonagh J

更新日期：1997-11-01 00:00:00

abstract：:Cytoplasmic proteins with binding capability to AU-rich instability determinant sequences (ARE) of tumour necrosis factor alpha (TNFalpha) mRNA 3' untranslated region (3'UTR) were assessed in human lymphoid cells. In vitro label transfer experiments using wild type as well as mutant sequences in which the 70 nucleotid...

journal_title：Cell biology international

authors： Henics T

更新日期：1999-01-01 00:00:00

abstract：:We investigated the effect of triptolide (TP) on spinal cord injury (SCI), and its underlying mechanism. Following the establishment of the SCI model using YFP H-line transgenic mice, TP was intraperitoneally injected at a dose of 0.2 mg/kg once daily for 7 days. Behavioral tests, Nissl staining, and hematoxylin-eosin...

journal_title：Cell biology international

authors： Zhu N,Ruan J,Yang X,Huang Y,Jiang Y,Wang Y,Cai D,Geng Y,Fang M

更新日期：2020-03-01 00:00:00

abstract：:At the end of transcardial perfusions with ice-cold physiological saline for 30 min or with isoosmotic potassium chloride for 5 min, but immediately before perfusion fixation, condenser-discharge electric shocks were administered to rats through surface electrodes pressed onto the temporal muscles of the scalped skull...

journal_title：Cell biology international

authors： Kellermayer R,Zsombok A,Auer T,Gallyas F

更新日期：2006-02-01 00:00:00

abstract：:Ca(2+)mobilization induced by ATP, isoproterenol and the Ca(2+)-ATPase inhibitor thapsigargin in the human submandibular duct cell line A253 was investigated using the Ca(2+)-sensitive fluorescent indicator fura-2. ATP and isoproterenol increased cytosolic free Ca(2+)([Ca(2+)](i)) and subsequent exposure to thapsigarg...

journal_title：Cell biology international

authors： Andersson C,Zhang AL,Roomans GM

更新日期：2000-01-01 00:00:00

abstract：:Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...

journal_title：Cell biology international

authors： Li L,Qi Y,Ma X,Xiong G,Wang L,Bao C

更新日期：2018-09-01 00:00:00

abstract：:The aim of the present study was to investigate stress fibers and cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment. Actin was studied by means of immunogold labeling, using the 'Progressive Lowering of Temperature' technique (PLT). Actin rearrangement was evaluated by a quantitative...

journal_title：Cell biology international

authors： Panagopoulou E,Kouloukoussa M,Kittas C,Marinos E

更新日期：2005-11-01 00:00:00