Abstract:
:The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and a control (nonsense) oligonucleotide specific for epsilon-chain of IgE (not expressing in HepG2 cells). Our data indicate that a gp130-specific ASO, g2, was the most effective blocker of IL-6-induced junB mRNA, whilst the IL-6 receptor ASOs alone were ineffective. The mechanism of gene inactivation by ASO treatment was partially elucidated by demonstration of the loss of gp130 mRNA from cells treated with ASOs showing functional efficacy. Our data may help to design antisense oligonucleotides that are effective in therapy (e.g. as anti-inflammatory agents) in the future.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus Adoi
10.1006/cbir.2001.0765keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
835-40issue
8eissn
1065-6995issn
1095-8355pii
S1065699501907656journal_volume
25pub_type
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