gp130-specific antisense oligonucleotides inhibit IL-6 signal inducing junB mRNA transcription in the human hepatoma cell line, HepG2.

Abstract:

:The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and a control (nonsense) oligonucleotide specific for epsilon-chain of IgE (not expressing in HepG2 cells). Our data indicate that a gp130-specific ASO, g2, was the most effective blocker of IL-6-induced junB mRNA, whilst the IL-6 receptor ASOs alone were ineffective. The mechanism of gene inactivation by ASO treatment was partially elucidated by demonstration of the loss of gp130 mRNA from cells treated with ASOs showing functional efficacy. Our data may help to design antisense oligonucleotides that are effective in therapy (e.g. as anti-inflammatory agents) in the future.

journal_name

Cell Biol Int

authors

Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus A

doi

10.1006/cbir.2001.0765

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

835-40

issue

8

eissn

1065-6995

issn

1095-8355

pii

S1065699501907656

journal_volume

25

pub_type

杂志文章
  • Discrepant effects of mammalian factors on molluscan cell motility, chemotaxis and phagocytosis: divergent evolution or finely tuned contingency?

    abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1042/CBI20100514

    authors: Malagoli D,Ottaviani E

    更新日期:2010-11-01 00:00:00

  • Interleukin-6-induced production of type II acute phase proteins and expression of junB gene are downregulated by human recombinant growth hormone in vitro.

    abstract::Growth hormone (GH), given therapeutically in many human diseases, is able to modulate the maturation and function of many cells of immune system. The present study demonstrates the effect of human recombinant GH on the production of acute phase proteins (APP) as well as on the gene expression of junB proto-oncogene o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0454

    authors: Derfalvi B,Igaz P,Fulop KA,Szalai C,Falus A

    更新日期:2000-01-01 00:00:00

  • K562 cells display different vulnerability to H₂O₂ induced oxidative stress in differing cell cycle phases.

    abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10360

    authors: Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

    更新日期:2015-02-01 00:00:00

  • Delineating cellular interactions between ciliates and fish by co-culturing Tetrahymena thermophila with fish cells.

    abstract::Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10310

    authors: Pinheiro MD,Bols NC

    更新日期:2014-10-01 00:00:00

  • Immunohistochemical and electron-microscopic identification of neuroendocrine cells in the stomach of uremic rats.

    abstract::Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morpho...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.03.012

    authors: Kasacka I

    更新日期:2004-01-01 00:00:00

  • Attenuation of Smad2 activity shows resistance to TGF-β signalling in mammary adenocarcinoma (MCF-7) cells.

    abstract::Transforming growth factor-β (TGF-β) is a potent inhibitor of the growth of normal mammary epithelial cells, and has a pleiotropic, context-dependent, concentration-dependent action. We found attenuation of TGF-β signalling in mammary adenoma carcinoma cells. Phosphorylation at the linker site of Smad2 occurred in a c...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10061

    authors: Sengupta S,Kundu S,Bhattacharyya A

    更新日期:2013-05-01 00:00:00

  • Hypoxia affects positively the proliferation of bovine satellite cells and their myogenic differentiation through up-regulation of MyoD.

    abstract::Hypoxia alters the biological functions of skeletal muscle cells to proliferate and differentiate into myotubes. However, the cellular responses of myoblasts to hypoxia differ according to the levels of oxygen and the types of cells studied. This study examined the effect of hypoxia (1% oxygen) on bovine satellite cel...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.03.017

    authors: Kook SH,Son YO,Lee KY,Lee HJ,Chung WT,Choi KC,Lee JC

    更新日期:2008-08-01 00:00:00

  • Adult peripheral blood mononuclear cells transdifferentiate in vitro and integrate into the retina in vivo.

    abstract::Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuc...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100146

    authors: Liu Q,Guan L,Huang B,Li W,Su Q,Yu M,Xu X,Luo T,Lin S,Sun X,Chen M,Chen X

    更新日期:2011-06-01 00:00:00

  • The effect of insulin on proteins associated with free, cytoskeletal-bound and membrane-bound polysome populations.

    abstract::It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1039

    authors: Moss R,Pryme IF,Vedeler A

    更新日期:1993-12-01 00:00:00

  • CJX1, an amlodipine derivative, interacts with ATPase of human P-glycoprotein.

    abstract::Our aim has been to elucidate the possible mechanism of CJX1, an amlodipine derivative, in the modulation of P-gp function by determining its effect on P-gp ATPase activity. Basal P-gp ATPase activity was increased by CJX1 with half-maximal activity concentration (Km) of 8.6+/-1.4 microM. Kinetic analysis indicated a ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2009.06.023

    authors: Ji BS,He L

    更新日期:2009-10-01 00:00:00

  • Hepatic differentiation of cord blood-derived multipotent progenitor cells (MPCs) in vitro.

    abstract::Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional ne...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.07.017

    authors: Moon YJ,Lee MW,Yoon HH,Yang MS,Jang IK,Lee JE,Kim HE,Eom YW,Park JS,Kim HC,Kim YJ,Lee KH

    更新日期:2008-10-01 00:00:00

  • Differentiation of embryonic stem cells towards pancreatic progenitor cells and their transplantation into streptozotocin-induced diabetic mice.

    abstract::Type I diabetes is characterized by the deficiency of endocrine beta cells in the pancreatic islets of Langerhans and transplantation of islet cells can be an effective therapeutic approach. Embryonic stem cells can be differentiated into any cell type, and therefore represent an unlimited source of islet cells for th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.12.017

    authors: Chen C,Zhang Y,Sheng X,Huang C,Zang YQ

    更新日期:2008-04-01 00:00:00

  • Hypoxia-induced apoptosis of astrocytes is mediated by reduction of Dicer and activation of caspase-1.

    abstract::Hypoxia is a condition in which the whole body or a region of the body is deprived of oxygen supply. The brain is very sensitive to the lack of oxygen and cerebral hypoxia can rapidly cause severe brain damage. Astrocytes are essential for the survival and function of neurons. Therefore, protecting astrocytes against ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11335

    authors: Kang YW,Kim YS,Park JY,Chu GE,Yang YC,Choi BY,Cho WG

    更新日期:2020-06-01 00:00:00

  • Metallothionein in human immunomagnetically selected CD34(+) haematopoietic progenitor cells.

    abstract::Human umbilical CD34(+) immature haematopoietic cells were rapidly and efficiently obtained from light density MNC (mononuclear cells) by MACS (magnetic cell sorting). An ex vivo expanded population of CD34(+) was cultured in serum-free medium supplemented with cytokines FL (flt3 ligand), SCF (stem cell factor) and TP...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090493

    authors: Maghdooni Bagheri P,De Ley M

    更新日期:2011-01-01 00:00:00

  • Interaction of plant polysomes with the actin cytoskeleton.

    abstract::Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Sev...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0517

    authors: Klyachko N,Aksenova L,Dunaeva M,Kulikova A

    更新日期:2000-01-01 00:00:00

  • Phallacidin stains the mitotic spindle of the diatom Pinnularia spp.

    abstract::Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.04.010

    authors: Sampson K

    更新日期:2004-01-01 00:00:00

  • Mycobacterial growth in human macrophages: variation according to donor, inoculum and bacterial strain.

    abstract::The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0679

    authors: Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PD

    更新日期:2001-01-01 00:00:00

  • Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres.

    abstract::The spatial distribution of acid membrane organelles and their relationships with normal and vacuolated transverse tubules has been studied in living frog skeletal muscle fibres using confocal microscopy. Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed t...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.06.017

    authors: Krolenko SA,Adamyan SY,Belyaeva TN,Mozhenok TP

    更新日期:2006-11-01 00:00:00

  • Human endometrial stem cells differentiation into functional hepatocyte-like cells.

    abstract::In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purificatio...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10278

    authors: Khademi F,Soleimani M,Verdi J,Tavangar SM,Sadroddiny E,Massumi M,Ai J

    更新日期:2014-07-01 00:00:00

  • Identification of circulating miRNA involved in meat yield of Korean cattle.

    abstract::Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10776

    authors: Lee S,Park SJ,Cheong JK,Ko JY,Bong J,Baik M

    更新日期:2017-07-01 00:00:00

  • FAM107B is regulated by S100A4 and mediates the effect of S100A4 on the proliferation and migration of MGC803 gastric cancer cells.

    abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10816

    authors: Guo J,Bian Y,Wang Y,Chen L,Yu A,Sun X

    更新日期:2017-10-01 00:00:00

  • Microbiological contamination in stem cell cultures.

    abstract::Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.03.010

    authors: Cobo F,Cortés JL,Cabrera C,Nieto A,Concha A

    更新日期:2007-09-01 00:00:00

  • Extended chromatin fibres: crystallinity, molecular order and reactivity to concanavalin-A.

    abstract::The present study was undertaken to test the reproducible formation of the extended chromatin fibres (ECF), beads and superbeads, and detect molecular order and crystallinity by optical anisotropies in those structures. Chicken erythrocyte smears and mouse liver cell imprints were treated with 2.0-3.0 m NaCl solution ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0555

    authors: de Campos Vidal B

    更新日期:2000-01-01 00:00:00

  • Zebularine significantly sensitises MEC1 cells to external irradiation and radiopharmaceutical therapy when administered sequentially in vitro.

    abstract::Zebularine is a cytidine analogue incorporated into DNA during replication, inhibiting DNA methyltransferase 1 (DNMT1), resulting in demethylation and changes in gene expression. Such modification may improve radiosensitivity in resistant lymphoma cells. The hypothesis of this study was that zebularine and radiation w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10215

    authors: Bryan JN,Kumar SR,Jia F,Balkin ER,Lewis MR

    更新日期:2014-02-01 00:00:00

  • Molecular identification of PpHDAC1, the first histone deacetylase fron the slime mold Physarum polycephalum.

    abstract::The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(02)90933-9

    authors: Brandtner EM,Lechner T,Loidl P,Lusser A

    更新日期:2002-01-01 00:00:00

  • Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

    abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1102

    authors: Andries L,Harrisson F,Vakaet L

    更新日期:1994-07-01 00:00:00

  • Retinal and olfactory bulb precursor cells show distinct responses to FGF-2 and laminin.

    abstract::We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated prol...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.031

    authors: Otaegi G,de Pablo F,Vicario-Abejón C,de la Rosa EJ

    更新日期:2007-07-01 00:00:00

  • Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa.

    abstract::Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10495

    authors: Li Y,Liu F,Zhang Z,Zhang M,Cao S,Li Y,Zhang L,Huang X,Xu Y

    更新日期:2015-11-01 00:00:00

  • Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3.

    abstract::The purpose of this study was three fold: (1) to reveal the implications of Wnt5A for cytokine and chemokine production by human ovarian cancer cell line SKOV-3 cells, (2) to determine the influence of Wnt5A on chemotactic SKOV-3 cell migration, and (3) to assess the effect of inflammatory mediators on Wnt5A expressio...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10551

    authors: Arabzadeh S,Hossein G,Zarnani AH

    更新日期:2016-02-01 00:00:00

  • Studies on the permeability of the blood-testis barrier in stainless steel-administered mice.

    abstract::Previous trials performed in our laboratory have demonstrated that stainless steel corrosion products induced morphological changes in mice seminiferous cells. In this study the functional nature of the blood-testis barrier was investigated, in view of the extensive use of this metallic alloy in orthopaedic surgery. T...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1109

    authors: Pereira ML

    更新日期:1995-07-01 00:00:00