Abstract：
:Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently becoming clear that cells will respond strongly to nano-scale topography. The fact that cells will take cues from their micro- and nano-environment suggests that the cells are in some way 'spatially aware'. It is likely that cells probe the shape of their surroundings using filopodia, and that this initial filopodia/topography interaction may be critical to down-stream cell reactions to biomaterials, or indeed, the extracellular matrix. One intriguing question is how small a feature can cells sense? In order to investigate the limits of cell sensing, high-resolution scanning electron microscopy has been used to simultaneously view cell filopodia and 10 nm high nano-islands. Fluorescence microscopy has also been used to look at adhesion formation. The results showed distinct filopodial/nano-island interaction and changes in adhesion morphology.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Dalby MJ,Riehle MO,Johnstone H,Affrossman S,Curtis ASdoi
10.1016/j.cellbi.2003.12.004keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
229-36issue
3eissn
1065-6995issn
1095-8355pii
S1065699504000058journal_volume
28pub_type
杂志文章abstract：:Neuroinflammation is closely related with the pathogenesis and progress of neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, has properties of inhibiting inflammation and improving memory. The present study was ai...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11060
更新日期：2018-12-01 00:00:00
abstract：:Non-specific L-type calcium channel blockers, such as verapamil (> or =50 microM), induce metaphase-II (M-II) arrest and apoptosis in aged rat eggs cultured in Ca(2+)-deficient medium. However, the effects of extracellular Ca(2+) on verapamil-induced M-II arrest and apoptosis have not yet been reported. We have demons...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2009.01.001
更新日期：2009-03-01 00:00:00
abstract：:Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2001.0778
更新日期：2001-01-01 00:00:00
abstract：:Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/s1065-6995(03)00157-4
更新日期：2003-01-01 00:00:00
abstract：:The focal adhesion protein vinculin has been implicated in associating with soluble and membranous phospholipids. Detailed investigations over the past ten years describe the intermolecular interactions of the vinculin tail domain with soluble and membrane phospholipids. Previous studies have implied that the tail's u...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10978
更新日期：2018-08-01 00:00:00
abstract：:TNF-α has long been implicated in the progression of rheumatoid arthritis (RA). However, how the receptors of TNF-α, namely TNFR1 and TNFR2, mediate TNF-α-induced inflammatory responses in fibroblast-like synoviocytes (FLS) in RA has not been elucidated. In the present study, primary FLS cells were isolated from RA pa...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10735
更新日期：2017-04-01 00:00:00
abstract：:Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.04.003
更新日期：2005-08-01 00:00:00
abstract：:Triple-negative breast cancer (TNBC) is a highly heterogeneous disease. The aim of this study is to identify the diagnostic and poor prognostic signatures in TNBC by exploring the aberrant DNA methylation and gene expression. Differential expression and methylation analysis of the TNBC and paracancer samples from The ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11406
更新日期：2020-10-01 00:00:00
abstract：:Metallothionein (MT), a cysteine-rich, metal-binding protein, is involved in homeostatic regulation of essential metals and protection of cells against oxidative injury. It has been shown that oxidative stress is associated with pathogenesis of osteoporosis and is capable of inhibiting osteoblastic differentiation of ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.09.004
更新日期：2004-01-01 00:00:00
abstract：:ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20090067
更新日期：2010-05-07 00:00:00
abstract：:Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11200
更新日期：2019-07-10 00:00:00
abstract：:The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transport...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2009.06.009
更新日期：2009-12-01 00:00:00
abstract：:Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. Th...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20090063
更新日期：2010-02-22 00:00:00
abstract：:Prolapsus uteri in pelvic supportive disorders are common in elderly women, and their etiology remains unclear. We examined elastin-binding proteins (EBPs) and binding sites in cultured cardinal ligament fibroblasts derived from elderly patients with prolapsus uteri (HPLiF) and compared them with those from age-matche...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2002.0877
更新日期：2002-01-01 00:00:00
abstract：:Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10258
更新日期：2014-06-01 00:00:00
abstract：:Ischemic stroke is a major common cause of death and long-term disability worldwide. Several pathophysiological events including excitotoxicity, oxidative/nitrative stress, inflammation, and apoptosis are involved in ischemic injuries. Recently, the molecular mechanisms involved in cerebral ischemia through a focus on...
journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1002/cbin.11237
更新日期：2020-02-01 00:00:00
abstract：:In this paper the radioresistance of human erythrocytes to radiation-induced haemolysis was studied by means of the split-dose technique. Human erythrocytes suspended in an isotonic Na-phosphatase buffer, pH 7.4 were exposed under air to gamma radiation. Erythrocytes were irradiated with a single dose of 2.7 kGy and w...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1997.0188
更新日期：1997-09-01 00:00:00
abstract：:We investigated the effect of triptolide (TP) on spinal cord injury (SCI), and its underlying mechanism. Following the establishment of the SCI model using YFP H-line transgenic mice, TP was intraperitoneally injected at a dose of 0.2 mg/kg once daily for 7 days. Behavioral tests, Nissl staining, and hematoxylin-eosin...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11273
更新日期：2020-03-01 00:00:00
abstract：:Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11015
更新日期：2018-09-01 00:00:00
abstract：:Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.04.002
更新日期：2008-09-01 00:00:00
abstract：:To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.09.012
更新日期：2005-02-01 00:00:00
abstract：:The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1996.0099
更新日期：1996-11-01 00:00:00
abstract：:The size and number of secretory granules in late larval salivary glands of Drosophila melanogaster have been related to interecdysial and early metamorphic development represented by well-known puffs in polytene chromosomes. Interecdysial period (puff stage 1 (PS1)) is characterized by presence of numerous small gran...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1999.0433
更新日期：1999-01-01 00:00:00
abstract：:Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs cons...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.04.025
更新日期：2008-09-01 00:00:00
abstract：:Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11445
更新日期：2020-12-01 00:00:00
abstract：:Imbalance of sexual steroids milieu and oxidative stress are often observed during aging and correlated to prostate disorders. Likewise, high-fat intake has been related to prostate damage and tumor development. Melatonin (MLT) is an antioxidant whose secretion decreases in elderly and is also suggested to protect the...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11472
更新日期：2021-01-01 00:00:00
abstract：:Restriction Endonucleases (REs) may recognize, cleave and remove DNA from fixed chromatin producing specific chromosome banding patterns. However, the modifications produced in the chromatin fibre are not easy to evaluate and compare. The aim of the present investigation was to visualize differences resulting in the t...
journal_title：Cell biology international
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1006/cbir.1996.0026
更新日期：1996-03-01 00:00:00
abstract：:Rosbin (Thieno [2, 3-c] piperidine-3-carboxamide-2-[(3-methoxy-naphtha-lene-2-carbonyl)-amino]-6-(benzyl)-, hydrochloride), a synthetic piperidinothieno derivative compound obtained from chemical genetics screenings, significantly suppresses the viability of non-small cell lung cancer A549 cells with an IC50 of 2.05 ±...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10714
更新日期：2017-02-01 00:00:00
abstract：:The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...
journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：
更新日期：1996-04-01 00:00:00
abstract：:Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20100314
更新日期：2011-02-01 00:00:00
